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cm q 1 0 0 1 225 0 cm q 188.3500061 0 0 -41.45000076 0 41.45000076 cm /X2 Do Q Q Q q 1 0 0 1 90 130.4940033 cm /P <</MCID 0 >> BDC BT /FAAABH 22 Tf 1 0 0 -1 0.168 23.67700005 Tm 2 Tr 0 g 0.62857145 w 0 G [(A)-4(M)-4(V)-4(/)-4(M)-4(M)-3(u)-4(L)-4(V)] TJ ET EMC /P <</Lang (zh-CN) /MCID 1 >> BDC BT /FAAABJ 22 Tf 1 0 0 -1 105.45999908 23.67700005 Tm [<0008>-4<0009>-4<000A>-4<000B>-4<000C>] TJ ET EMC /P <</MCID 2 >> BDC BT /FAAABH 22 Tf 1 0 0 -1 215.8999939 23.67700005 Tm [(\()-4(1)-4(0)] TJ ET EMC /P <</Lang (zh-CN) /MCID 3 >> BDC BT 1 0 0 -1 249.16400146 23.67700005 Tm [(?] TJ ET EMC /P <</MCID 4 >> BDC BT 1 0 0 -1 271.25201416 23.67700005 Tm [(,)-4(p)-4(H)-4(8)-4(.)-4(3)-3(\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 5 >> BDC BT /FAAABJ 22 Tf 1 0 0 -1 348.86801147 23.67700005 Tm [<0018>-4<0019>-4<001A>] TJ ET EMC /P <</MCID 6 >> BDC BT /FAAABH 22 Tf 1 0 0 -1 415.13198853 23.67700005 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC q 1 0 0 1 94.5 31.20000076 cm /P <</MCID 7 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 160.3999939 11.65499973 Tm 0.30000001 w [( )] TJ ET EMC Q q 1 0 0 1 0 46.79999924 cm /P <</Lang (zh-CN) /MCID 8 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 102.23000336 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<001B>-4<001C>-4<001D>-4<001E>-4<001F>-4<0020>-3<0021>-4<0022>-4<0023>-4<0024>-4<0025>-4<0026>-4<0027>-4<0028>-4<0025>-4<0029>-3<002A>-4<002B>-4<002C>-4<002D>] TJ ET EMC /P <</MCID 9 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 313.07000732 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 62.40000153 cm /P <</MCID 10 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 78 cm /P <</MCID 11 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 93.59999847 cm /P <</Lang (zh-CN) /MCID 12 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<001C>-4<001D>-4<002E>-4<002F>-4<0030>] TJ ET EMC /P <</MCID 13 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 109.19999695 cm /P <</MCID 14 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(Tris\()] TJ ET EMC /P <</Lang (zh-CN) /MCID 15 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 26.25 11.65499973 Tm [<0035003600370038003900380037003A>] TJ ET EMC /P <</MCID 16 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 110.25 11.65499973 Tm [(\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 17 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 115.5 11.65499973 Tm [<003B003C003D0026003E003F0040003B>] TJ ET EMC /P <</MCID 18 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 199.5 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 19 >> BDC BT 1 0 0 -1 204.75 11.65499973 Tm [(C4H11NO3)] TJ ET EMC /P <</Lang (zh-CN) /MCID 20 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 246.75 11.65499973 Tm [<002600450046003E003F0047003B>] TJ ET EMC /P <</MCID 21 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 320.25 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 22 >> BDC BT 1 0 0 -1 325.5 11.65499973 Tm [(121.14,)] TJ ET EMC /P <</MCID 23 >> BDC BT 1 0 0 -1 362.25 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 24 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 367.5 11.65499973 Tm [<0049>] TJ ET EMC /P <</MCID 25 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 378 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 26 >> BDC BT 1 0 0 -1 383.25 11.65499973 Tm [(25)] TJ ET EMC /P <</Lang (zh-CN) /MCID 27 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 393.75 11.65499973 Tm [<004B>] TJ ET EMC /P <</MCID 28 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 404.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 124.80000305 cm /P <</Lang (zh-CN) /MCID 29 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [<004C>] TJ ET EMC /P <</MCID 30 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 13.125 11.65499973 Tm [(pKa)] TJ ET EMC /P <</MCID 31 >> BDC BT 1 0 0 -1 28.875 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 32 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 34.125 11.65499973 Tm [<003B>] TJ ET EMC /P <</MCID 33 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 44.625 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 34 >> BDC BT 1 0 0 -1 49.875 11.65499973 Tm [(8.1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 35 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 65.625 11.65499973 Tm [<0026>] TJ ET EMC /P <</MCID 36 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 75.08799744 11.65499973 Tm [(Tris)] TJ ET EMC /P <</MCID 37 >> BDC BT 1 0 0 -1 96.08799744 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 38 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 101.33799744 11.65499973 Tm [<000A000B000C004F00500051000A000B005200530049>] TJ ET EMC /P <</MCID 39 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 216.83799744 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 40 >> BDC BT 1 0 0 -1 222.08799744 11.65499973 Tm [(pH7.0)] TJ ET EMC /P <</Lang (zh-CN) /MCID 41 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 248.33799744 11.65499973 Tm [<0055>] TJ ET EMC /P <</MCID 42 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 258.8380127 11.65499973 Tm [(9.2)] TJ ET EMC /P <</MCID 43 >> BDC BT 1 0 0 -1 274.5880127 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 44 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 279.8380127 11.65499973 Tm [<00570058>] TJ ET EMC /P <</Lang (zh-CN) /MCID 45 >> BDC BT 1 0 0 -1 300.8380127 11.65499973 Tm [<0026>] TJ ET EMC /P <</Lang (zh-CN) /MCID 46 >> BDC BT 1 0 0 -1 310.30099487 11.65499973 Tm [<00250059005A003B005B005C000A000B000C004F>] TJ ET EMC /P <</Lang (zh-CN) /MCID 47 >> BDC BT 1 0 0 -1 0 27.25500107 Tm [<0038>-21<005D>-21<005E>-22<003E>-21<0026>-21<005F>-21<0060>-22<0061>-21<003D>-21<0062>-21<0063>] TJ ET EMC /P <</MCID 48 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 117.95300293 27.25500107 Tm [(AMV/MMuLV)] TJ ET EMC /P <</MCID 49 >> BDC BT 1 0 0 -1 165.20300293 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 50 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 170.67599487 27.25500107 Tm [<0008>-21<0009>-21<000A>-22<000B>-21<000C>] TJ ET EMC /P <</MCID 51 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 224.29100037 27.25500107 Tm [(\(10)] TJ ET EMC /P <</Lang (zh-CN) /MCID 52 >> BDC BT 1 0 0 -1 240.26400757 27.25500107 Tm [(?] TJ ET EMC /P <</MCID 53 >> BDC BT 1 0 0 -1 250.98699951 27.25500107 Tm [(,pH8.3\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 54 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 287.95999146 27.25500107 Tm [<0064>] TJ ET EMC /P <</MCID 55 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 298.68301392 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 56 >> BDC BT 1 0 0 -1 304.15600586 27.25500107 Tm [(100mM)] TJ ET EMC /P <</MCID 57 >> BDC BT 1 0 0 -1 330.40600586 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 58 >> BDC BT 1 0 0 -1 335.8789978 27.25500107 Tm [(Tris-HCl)] TJ ET EMC /P <</MCID 59 >> BDC BT 1 0 0 -1 377.8789978 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 60 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 383.35198975 27.25500107 Tm [<000A>-21<000B>-21<000C>] TJ ET EMC /P <</MCID 61 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 42.85499954 Tm [(\(pH8.3\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 62 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 36.75 42.85499954 Tm [<0068>] TJ ET EMC /P <</MCID 63 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 46.07500076 42.85499954 Tm [(15mMMg2+)] TJ ET EMC /P <</Lang (zh-CN) /MCID 64 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 88.07499695 42.85499954 Tm [<0068>] TJ ET EMC /P <</MCID 65 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 97.40000153 42.85499954 Tm [(500mM)] TJ ET EMC /P <</MCID 66 >> BDC BT 1 0 0 -1 123.65000153 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 67 >> BDC BT 1 0 0 -1 128.8999939 42.85499954 Tm [(KCl)] TJ ET EMC /P <</MCID 68 >> BDC BT 1 0 0 -1 144.6499939 42.85499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 69 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 149.8999939 42.85499954 Tm [<006B006C005E>] TJ ET EMC /P <</Lang (zh-CN) /MCID 70 >> BDC BT 1 0 0 -1 181.3999939 42.85499954 Tm [<0026>] TJ ET EMC /P <</Lang (zh-CN) /MCID 71 >> BDC BT 1 0 0 -1 190.7250061 42.85499954 Tm [<006D>] TJ ET EMC /P <</MCID 72 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 201.2250061 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 73 >> BDC BT 1 0 0 -1 206.4750061 42.85499954 Tm [(RNase)] TJ ET EMC /P <</MCID 74 >> BDC BT 1 0 0 -1 232.7250061 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 75 >> BDC BT 1 0 0 -1 237.9750061 42.85499954 Tm [(free)] TJ ET EMC /P <</MCID 76 >> BDC BT 1 0 0 -1 258.9750061 42.85499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 77 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 264.2250061 42.85499954 Tm [<00710072007300740075>] TJ ET EMC /P <</Lang (zh-CN) /MCID 78 >> BDC BT 1 0 0 -1 316.7250061 42.85499954 Tm [<0026>] TJ ET EMC /P <</Lang (zh-CN) /MCID 79 >> BDC BT 1 0 0 -1 326.04998779 42.85499954 Tm [<005A003B>] TJ ET EMC /P <</MCID 80 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 347.04998779 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 81 >> BDC BT 1 0 0 -1 352.29998779 42.85499954 Tm [(AMV)] TJ ET EMC /P <</MCID 82 >> BDC BT 1 0 0 -1 368.04998779 42.85499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 83 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 373.29998779 42.85499954 Tm [<0076>] TJ ET EMC /P <</MCID 84 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 383.79998779 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 85 >> BDC BT 1 0 0 -1 389.04998779 42.85499954 Tm [(MMuLV)] TJ ET EMC /P <</MCID 86 >> BDC BT 1 0 0 -1 415.29998779 42.85499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 87 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 0 58.45499802 Tm [<006B007700780079007A004F00080009000A000B000C0063007B007C007D001E00250029007E0022007F008000260027008100250029002A002B002C002D007600820083002500840063>] TJ ET EMC /P <</MCID 88 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 388.5 58.45499802 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 187.19999695 cm /P <</MCID 89 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 202.80000305 cm /P <</Lang (zh-CN) /MCID 90 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<001C>-4<001D>-4<006C>-4<005E>-4<0030>] TJ ET EMC /P <</MCID 91 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 385.6499939 218.3999939 cm /P <</MCID 92 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 385.6499939 234 cm /P <</MCID 93 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 385.6499939 249.6000061 cm /P <</MCID 94 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC /P <</MCID 95 >> BDC BT 1 0 0 -1 0 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 96 >> BDC BT 1 0 0 -1 21 27.25500107 Tm [( )] TJ ET EMC Q q 1 0 0 1 385.6499939 280.79998779 cm /P <</MCID 97 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 296.3999939 cm /P <</MCID 98 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 312 cm /P <</Lang (zh-CN) /MCID 99 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<0085>-4<005A>-4<0086>-4<0087>] TJ ET EMC /P <</MCID 100 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 42.16799927 11.65499973 Tm [(\()] TJ ET EMC /P <</Lang (zh-CN) /MCID 101 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 47.45999908 11.65499973 Tm [<001E>-4<001F>-4<0088>-4<0089>] TJ ET EMC /P <</MCID 102 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 89.62799835 11.65499973 Tm [(\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 103 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 94.91999817 11.65499973 Tm [<0030>] TJ ET EMC /P <</MCID 104 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 105.46199799 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 327.6000061 cm /P <</MCID 105 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 106 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<0068008A008B008C008D0020008E008F0085005A0063>] TJ ET EMC /P <</MCID 107 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 120.75 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 343.20001221 cm /P <</MCID 108 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(2)] TJ ET EMC /P <</Lang (zh-CN) /MCID 109 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<0068>] TJ ET EMC /P <</MCID 110 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 15.75 11.65499973 Tm [(AMV/MMuLV)] TJ ET EMC /P <</MCID 111 >> BDC BT 1 0 0 -1 63 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 112 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 68.25 11.65499973 Tm [<00080009000A000B000C>] TJ ET EMC /P <</MCID 113 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 120.75 11.65499973 Tm [(\(10)] TJ ET EMC /P <</Lang (zh-CN) /MCID 114 >> BDC BT 1 0 0 -1 136.5 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 115 >> BDC BT 1 0 0 -1 147 11.65499973 Tm [(,pH8.3\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 116 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 183.75 11.65499973 Tm [<0090005A00910062003B>] TJ ET EMC /P <</MCID 117 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 236.25 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 118 >> BDC BT 1 0 0 -1 241.5 11.65499973 Tm [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 119 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 246.75 11.65499973 Tm [<00100063>] TJ ET EMC /P <</MCID 120 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 267.75 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 358.79998779 cm /P <</MCID 121 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 374.3999939 cm /P <</Lang (zh-CN) /MCID 122 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<0092>-4<0093>-4<0094>-4<0095>-4<0030>] TJ ET EMC /P <</MCID 123 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 390 cm /P <</MCID 124 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 125 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<00680092009300960097000800980099009A0026009B009C009D009E0051009F00A000A1004C00A20063>] TJ ET EMC /P <</MCID 126 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 225.75 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 405.6000061 cm /P <</MCID 127 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(2)] TJ ET EMC /P <</Lang (zh-CN) /MCID 128 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<00680092009300960097>] TJ ET EMC /P <</MCID 129 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 57.75 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 130 >> BDC BT 1 0 0 -1 63 11.65499973 Tm [(RNase)] TJ ET EMC /P <</MCID 131 >> BDC BT 1 0 0 -1 89.25 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 132 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 94.5 11.65499973 Tm [<00A300A4002600A500A60096009700A700A800A900A300A40063>] TJ ET EMC /P <</MCID 133 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 231 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 421.20001221 cm /P <</MCID 134 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(3)] TJ ET EMC /P <</Lang (zh-CN) /MCID 135 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<0068003B00AA00AB004F00AC00AD007100AE00AF002600B000B1008B008C00B200A500B300B400B500B600B700B80085005A0063>] TJ ET EMC /P <</MCID 136 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 278.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 436.79998779 cm /P <</MCID 137 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 452.3999939 cm /P <</Lang (zh-CN) /MCID 138 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<0045>-4<00B9>-4<001C>-4<001D>-4<0030>] TJ ET EMC /P <</MCID 139 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 468 cm /P <</MCID 140 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 1.79999995 483.6000061 cm q 0.25 0.5 m 374 0.5 l 374 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 141 >> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(DMSO\(PCR)] TJ ET EMC /P <</Lang (zh-CN) /MCID 142 >> BDC BT /FAAACH 11 Tf 1 0 0 -1 46.75 11.83899975 Tm [<00BD>] TJ ET EMC /P <</MCID 143 >> BDC BT /FAAACE 11 Tf 1 0 0 -1 57.75 11.83899975 Tm [(\))] TJ ET EMC /P <</MCID 144 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 63.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 16.10000038 cm q 0.25 0.5 m 374 0.5 l 374 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 145 >> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(DNA)] TJ ET EMC /P <</Lang (zh-CN) /MCID 146 >> BDC BT /FAAACH 11 Tf 1 0 0 -1 19.25 11.83899975 Tm [<00BE00BF000A000B000C>] TJ ET EMC /P <</MCID 147 >> BDC BT /FAAACE 11 Tf 1 0 0 -1 74.25 11.83899975 Tm [(\(5)] TJ ET EMC /P <</Lang (zh-CN) /MCID 148 >> BDC BT 1 0 0 -1 85.25 11.83899975 Tm [(?] TJ ET EMC /P <</MCID 149 >> BDC BT 1 0 0 -1 96.25 11.83899975 Tm [(\))] TJ ET EMC /P <</MCID 150 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 101.75 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 32.20000076 cm q 0.25 0.5 m 374 0.5 l 374 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 151 >> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(EB)] TJ ET EMC /P <</Lang (zh-CN) /MCID 152 >> BDC BT /FAAACH 11 Tf 1 0 0 -1 13.75 11.83899975 Tm [<00C200C3000C>] TJ ET EMC /P <</MCID 153 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 46.75 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 48.29999924 cm q 0.25 0.5 m 374 0.5 l 374 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 154 >> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(EX+Taq+DNA+Polymerase)] TJ ET EMC /P <</MCID 155 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 115.5 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 64.40000153 cm q 0.25 0.5 m 374 0.5 l 374 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 156 >> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(PCR)] TJ ET EMC /P <</Lang (zh-CN) /MCID 157 >> BDC BT /FAAACH 11 Tf 1 0 0 -1 19.25 11.83899975 Tm [<009D009E000A000B000C>] TJ ET EMC /P <</MCID 158 >> BDC BT /FAAACE 11 Tf 1 0 0 -1 74.25 11.83899975 Tm [(\(10)] TJ ET EMC /P <</Lang (zh-CN) /MCID 159 >> BDC BT 1 0 0 -1 90.75 11.83899975 Tm [(?] TJ ET EMC /P <</MCID 160 >> BDC BT 1 0 0 -1 101.75 11.83899975 Tm [(,pH8.3\))] TJ ET EMC /P <</MCID 161 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 140.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 80.5 cm q 0.25 0.5 m 374 0.5 l 374 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 162 >> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(Taq+DNA+Polymerase)] TJ ET EMC /P <</MCID 163 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 99 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q Q q /GS2 gs 0 G 0.5 w 0 0.5 m 0 96.59999847 l S Q q /GS2 gs 0 G 0.5 w 374.25 0.5 m 374.25 96.59999847 l S Q q /GS2 gs 0 G 0.5 w -0.25 0.25 m 374.5 0.25 l S Q q /GS2 gs 0 G 0.5 w 0.25 16.35000038 m 374 16.35000038 l S Q q /GS2 gs 0 G 0.5 w 0.25 32.45000076 m 374 32.45000076 l S Q q /GS2 gs 0 G 0.5 w 0.25 48.55000305 m 374 48.55000305 l S Q q /GS2 gs 0 G 0.5 w 0.25 64.65000153 m 374 64.65000153 l S Q q /GS2 gs 0 G 0.5 w 0.25 80.75 m 374 80.75 l S Q q /GS2 gs 0 G 0.5 w -0.25 96.84999847 m 374.5 96.84999847 l S Q Q q 1 0 0 1 0 580.70001221 cm /P <</MCID 164 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 596.29998779 cm Q Q q 1 0 0 1 90.55000305 358.44400024 cm q 0.25 0.5 m 165.25 0.5 l 165.25 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 165 >> BDC BT /FAAACH 9 Tf 1 0 0 -1 59.34999847 15.00500011 Tm 0 g [<001C001D00C800C9>] TJ ET EMC /P <</MCID 166 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 95.34999847 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 165.5 0 cm 0.25 0.5 m 56 0.5 l 56 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 167 >> BDC BT /FAAACH 9 Tf 1 0 0 -1 13.72500038 15.00500011 Tm 0 g [<00CA00CB>] TJ ET EMC /P <</MCID 168 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 31.72500038 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 221.75 0 cm 0.25 0.5 m 44.15000153 0.5 l 44.15000153 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 169 >> BDC BT /FAAACH 9 Tf 1 0 0 -1 3.29999995 15.00500011 Tm 0 g [<00180019001A>] TJ ET EMC /P <</MCID 170 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 30.29999924 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 266.1499939 0 cm 0.25 0.5 m 55.45000076 0.5 l 55.45000076 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 171 >> BDC BT /FAAACH 9 Tf 1 0 0 -1 8.94999981 15.00500011 Tm 0 g [<0050005100CC>] TJ ET EMC /P <</MCID 172 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 35.95000076 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 321.8500061 0 cm 0.25 0.5 m 53.75 0.5 l 53.75 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 173 >> BDC BT /FAAACH 9 Tf 1 0 0 -1 3.5999999 15.00500011 Tm 0 g [<00CD00CE00CF00D0>] TJ ET EMC /P <</MCID 174 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 39.59999847 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 23.89999962 cm q 0.25 0.5 m 165.25 0.5 l 165.25 28.60000038 l 0.25 28.60000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 175 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 3.57399988 17.35499954 Tm 0 g [(AMV/MMu)-1(LV)] TJ ET EMC /P <</MCID 176 >> BDC BT 1 0 0 -1 54.06700134 17.35499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 177 >> BDC BT /FAAACH 9 Tf 1 0 0 -1 58.56700134 17.35499954 Tm [<00080009000A000B000C>] TJ ET EMC /P <</MCID 178 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 103.56700134 17.35499954 Tm [(\(10)] TJ ET EMC /P <</Lang (zh-CN) /MCID 179 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 115.41899872 17.35499954 Tm [(?] TJ ET EMC /P <</MCID 180 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 124.41899872 17.35499954 Tm [(,pH8.3\))] TJ ET EMC /P <</MCID 181 >> BDC BT 1 0 0 -1 151.125 17.35499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 165.5 0 cm 0.25 0.5 m 56 0.5 l 56 28.60000038 l 0.25 28.60000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 182 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 13.72500038 17.35499954 Tm 0 g [(10ml)] TJ ET EMC /P <</MCID 183 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 31.72500038 17.35499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 221.75 0 cm 0.25 0.5 m 44.15000153 0.5 l 44.15000153 28.60000038 l 0.25 28.60000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 184 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 8.89400005 17.35499954 Tm 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 185 >> BDC BT /FAAACH 9 Tf 1 0 0 -1 15.70600033 17.35499954 Tm [<00D1>] TJ ET EMC /P <</MCID 186 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 24.70599937 17.35499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 266.1499939 0 cm 0.25 0.5 m 55.45000076 0.5 l 55.45000076 28.60000038 l 0.25 28.60000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 187 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 7.76300001 17.35499954 Tm 0 g [(12)] TJ ET EMC /P <</Lang (zh-CN) /MCID 188 >> BDC BT /FAAACH 9 Tf 1 0 0 -1 19.13599968 17.35499954 Tm [<00D200D3>] TJ ET EMC /P <</MCID 189 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 37.13600159 17.35499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 321.8500061 0 cm 0.25 0.5 m 53.75 0.5 l 53.75 28.60000038 l 0.25 28.60000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 190 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 10.35000038 17.35499954 Tm 0 g [(-20)] TJ ET EMC /P <</Lang (zh-CN) /MCID 191 >> BDC BT /FAAACH 9 Tf 1 0 0 -1 23.85000038 17.35499954 Tm [<004B>] TJ ET EMC /P <</MCID 192 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 32.84999847 17.35499954 Tm [( )] TJ ET EMC /P <</MCID 193 >> BDC BT 1 0 0 -1 37.34999847 17.35499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0 G 0.5 w 0 0.5 m 0 52.5 l S Q q 0 G 0.5 w 165.5 0.5 m 165.5 23.89999962 l S Q q 0 G 0.5 w 165.5 24.39999962 m 165.5 52.5 l S Q q 0 G 0.5 w 221.75 0.5 m 221.75 23.89999962 l S Q q 0 G 0.5 w 221.75 24.39999962 m 221.75 52.5 l S Q q 0 G 0.5 w 266.1499939 0.5 m 266.1499939 23.89999962 l S Q q 0 G 0.5 w 266.1499939 24.39999962 m 266.1499939 52.5 l S Q q 0 G 0.5 w 321.8500061 0.5 m 321.8500061 23.89999962 l S Q q 0 G 0.5 w 321.8500061 24.39999962 m 321.8500061 52.5 l S Q q 0 G 0.5 w 375.8500061 0.5 m 375.8500061 52.5 l S Q q 0 G 0.5 w -0.25 0.25 m 376.1000061 0.25 l S Q q 0 G 0.5 w 0.25 24.14999962 m 375.6000061 24.14999962 l S Q q 0 G 0.5 w -0.25 52.75 m 376.1000061 52.75 l S Q Q 1 0 0 -1 0 841.90002441 cm 
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