%PDF-1.7 %ÈÈÈÈÈÈ?1 0 obj <> endobj 2 0 obj <>/Metadata 122 0 R>> endobj 3 0 obj <> endobj 4 0 obj <>/ExtGState<>/XObject<>>>/Group<>/StructParents 0/Tabs/S>> endobj 5 0 obj <>stream 1 0 0 -1 0 841.90002441 cm q 1 0 0 1 65.65000153 94.18599701 cm q 474.8500061 0 0 -20.80000114 0 20.80000114 cm /X1 Do Q Q /Artifact <> BDC q 1 0 0 1 90 42.54999924 cm -90 0 m 505.29998779 0 l 505.29998779 87.94400024 l -90 87.94400024 l h W n BT /FAAAAI 9 Tf 1 0 0 -1 0 9.56900024 Tm 0 g [( )-1( )-1( )-1( )] TJ /GS1 gs 0 g [-11231( )] TJ ET q 1 0 0 1 0 11.98600006 cm BT 1 0 0 -1 0 42 Tm /GS2 gs 0 g [( )-1( )-1( )-1( )-1( )] TJ 1 0 0 -1 414 42 Tm /GS1 gs 0 g [( )] TJ ET Q q 1 0 0 1 0 53.98600006 cm BT 1 0 0 -1 0 8.56900024 Tm /GS2 gs 0 g [( )-1( )-1( )] TJ /GS1 gs 0 g [-9040( )] TJ ET Q q 1 0 0 1 0 64.97200012 cm BT 1 0 0 -1 0 8.56900024 Tm [( )] TJ ET Q q 1 0 0 1 0 75.95800018 cm BT 1 0 0 -1 0 8.56900024 Tm /GS2 gs 0 g [( )] TJ /FAAABD 9 Tf [-548( )] TJ ET Q Q EMC q 1 0 0 1 90 54.5359993 cm q 1 0 0 1 225 0 cm q 188.3500061 0 0 -41.45000076 0 41.45000076 cm /X2 Do Q Q Q q 1 0 0 1 90 130.4940033 cm /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 0 23.67700005 Tm 2 Tr 0 g 0.62857145 w 0 G [(T)-4(w)-4(e)-4(e)-4(n)-4(2)-3(0)-4(/)-4(T)-4(B)-4(S)] TJ ET EMC /P <> BDC BT /FAAABJ 22 Tf 1 0 0 -1 127.46800232 23.67700005 Tm [<000C>-4<000D>] TJ ET EMC /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 171.64399719 23.67700005 Tm [(\()-4(1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 193.82000732 23.67700005 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 215.90800476 23.67700005 Tm [(T)-4(B)-4(S)-4(T)-4(,)-4(p)-3(H)-4(7)-4(.)-4(4)-4(\))] TJ ET EMC /P <> BDC BT /FAAABJ 22 Tf 1 0 0 -1 337.87600708 23.67700005 Tm [<0018>-4<0019>-4<001A>] TJ ET EMC /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 404.14001465 23.67700005 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC q 1 0 0 1 94.5 31.20000076 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 160.3999939 11.65499973 Tm 0.30000001 w [( )] TJ ET EMC Q q 1 0 0 1 73.5 46.79999924 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<001B>-4<001C>-4<001D>-4<001E>-4<001F>-4<0020>-3<0021>-4<0022>-4<0023>-4<0024>-4<0025>-4<0026>-4<0027>-4<0028>-4<0025>-4<0029>-3<002A>-4<002B>-4<002C>-4<002D>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 210.83999634 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 62.40000153 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 78 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<001C>-4<001D>-4<002E>-4<002F>-4<0030>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 93.59999847 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(Tris)] TJ ET EMC /P <> BDC BT 1 0 0 -1 21 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 23.625 11.65499973 Tm [<003400350036000C000D002E0037>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 97.125 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 99.75 11.65499973 Tm [(TBS,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 120.75 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 123.375 11.65499973 Tm [<003800290039003A>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 165.375 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 168 11.65499973 Tm [(pH)] TJ ET EMC /P <> BDC BT 1 0 0 -1 178.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 181.125 11.65499973 Tm [<00340035000D>] TJ ET EMC /P <> BDC BT 1 0 0 -1 212.625 11.65499973 Tm [<0026>] TJ ET EMC /P <> BDC BT 1 0 0 -1 218.42500305 11.65499973 Tm [<003900250029003B003C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 270.92498779 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 273.54998779 11.65499973 Tm [(Western)] TJ ET EMC /P <> BDC BT 1 0 0 -1 310.29998779 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 312.92498779 11.65499973 Tm [(Blot)] TJ ET EMC /P <> BDC BT 1 0 0 -1 333.92498779 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 336.54998779 11.65499973 Tm [<004100420043004400450046>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 399.54998779 11.65499973 Tm [(,)] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 404.79998779 11.65499973 Tm [<0047>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [<00480049004A004B004C000D>] TJ ET EMC /P <> BDC BT 1 0 0 -1 63 27.25500107 Tm [<0026>] TJ ET EMC /P <> BDC BT 1 0 0 -1 71.93399811 27.25500107 Tm [<004D00390025004E004F00500051005200530054>] TJ ET EMC /P <> BDC BT 1 0 0 -1 176.93400574 27.25500107 Tm [<0055>] TJ ET EMC /P <> BDC BT 1 0 0 -1 185.86799622 27.25500107 Tm [<0056>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 198.99299622 27.25500107 Tm [(TBS)] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 217.36799622 27.25500107 Tm [<005700580059005A005B005C005D005E005F00600057>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 332.86801147 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 335.49200439 27.25500107 Tm [(Tween)] TJ ET EMC /P <> BDC BT 1 0 0 -1 361.74200439 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 364.36599731 27.25500107 Tm [(20)] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 374.86599731 27.25500107 Tm [<0026>] TJ ET EMC /P <> BDC BT 1 0 0 -1 383.79998779 27.25500107 Tm [<006100620063>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 42.85499954 Tm [<006300640065>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 31.5 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 36.75 42.85499954 Tm [(TBS)] TJ ET EMC /P <> BDC BT 1 0 0 -1 52.5 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 57.75 42.85499954 Tm [<0057003C0066006700250055>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 120.75 42.85499954 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 140.3999939 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(Tween20/TBS)] TJ ET EMC /P <> BDC BT 1 0 0 -1 57.75 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 63 11.65499973 Tm [<000C000D>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 84 11.65499973 Tm [(\(1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 94.5 11.65499973 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 105 11.65499973 Tm [(TBST,pH7.4\))] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 162.75 11.65499973 Tm [<00680069006A004E006B>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 215.25 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 220.5 11.65499973 Tm [(Tris-HCl\(pH7.4\))] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 299.25 11.65499973 Tm [<006E>] TJ ET EMC /P <> BDC BT 1 0 0 -1 307.3999939 11.65499973 Tm [<006F00700071>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 338.8999939 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 344.1499939 11.65499973 Tm [(Tween)] TJ ET EMC /P <> BDC BT 1 0 0 -1 370.3999939 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 375.6499939 11.65499973 Tm [(20)] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 386.1499939 11.65499973 Tm [<006E>] TJ ET EMC /P <> BDC BT 1 0 0 -1 394.29998779 11.65499973 Tm [<00720073>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [<005400740026>] TJ ET EMC /P <> BDC BT 1 0 0 -1 31.5 27.25500107 Tm [<0068006900250029>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 76.125 27.25500107 Tm [(Western)] TJ ET EMC /P <> BDC BT 1 0 0 -1 112.875 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 118.125 27.25500107 Tm [(Blot)] TJ ET EMC /P <> BDC BT 1 0 0 -1 139.125 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 144.375 27.25500107 Tm [<0041>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 157.5 27.25500107 Tm [(PVDF)] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 178.5 27.25500107 Tm [<006E>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 189 27.25500107 Tm [(NC)] TJ ET EMC /P <> BDC BT 1 0 0 -1 199.5 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 204.75 27.25500107 Tm [<00460057003C00660026007A007B0067006B004B004C000D005700580059005300540055>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 393.75 27.25500107 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 171.6000061 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 187.19999695 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<001C>-4<001D>-4<007C>-4<006A>-4<0030>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 381.6499939 202.80000305 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 381.6499939 218.3999939 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 381.6499939 234 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 381.6499939 249.6000061 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 265.20001221 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 21 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 42 11.65499973 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 280.79998779 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<007D>-4<0067>-4<007E>-4<007F>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 42.16799927 11.65499973 Tm [(\()] TJ ET EMC /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 47.45999908 11.65499973 Tm [<001E>-4<001F>-4<0080>-4<0081>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 89.62799835 11.65499973 Tm [(\))] TJ ET EMC /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 94.91999817 11.65499973 Tm [<0030>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 105.46199799 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 296.3999939 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(1)] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<006E00820083008400850086002000690087007D00670055>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 131.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 312 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(2)] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<006E003B003C0046008800260089008A>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 89.25 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 94.5 11.65499973 Tm [(5)] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 99.75 11.65499973 Tm [<008C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 110.25 11.65499973 Tm [(10min)] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 136.5 11.65499973 Tm [<0026008E008F>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 168 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 173.25 11.65499973 Tm [(3)] TJ ET EMC /P <> BDC BT 1 0 0 -1 178.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 183.75 11.65499973 Tm [<008A0055>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 204.75 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 327.6000061 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 343.20001221 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<0091>-4<0092>-4<0093>-4<0094>-4<0030>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 358.79998779 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(1)] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<006E009500960089008A0057002400250097009800990026007B009A009B009C004E009D009E009F002400250055>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 246.75 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 374.3999939 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr [(2)] TJ ET EMC /P <> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<006E006B00A000A1005700A200A300A400A500A6002600A700A80084008500A900AA00AB005D008A00AC00AD00AE007D00670055>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 278.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 390 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 405.6000061 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0.30000001 w /GS2 gs 0 G [<00AF>-4<00B0>-4<001C>-4<001D>-4<0030>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 421.20001221 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 1.79999995 436.79998779 cm q 0.25 0.5 m 369.5 0.5 l 369.5 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(TruPAGE)] TJ ET EMC /P <> BDC BT /FAAACH 11 Tf 1 0 0 -1 41.25 11.83899975 Tm [<00B400B500340035000D>] TJ ET EMC /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 96.25 11.83899975 Tm [(\(20)] TJ ET EMC /P <> BDC BT 1 0 0 -1 112.75 11.83899975 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 123.75 11.83899975 Tm [(\))] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 129.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 16.10000038 cm q 0.25 0.5 m 369.5 0.5 l 369.5 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(Tris)] TJ ET EMC /P <> BDC BT /FAAACH 11 Tf 1 0 0 -1 24.75 11.83899975 Tm [<003400350036000C000D>] TJ ET EMC /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 79.75 11.83899975 Tm [(\(20)] TJ ET EMC /P <> BDC BT 1 0 0 -1 96.25 11.83899975 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 107.25 11.83899975 Tm [(TBS,pH8.0\))] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 162.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 32.20000076 cm q 0.25 0.5 m 369.5 0.5 l 369.5 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(Tris)] TJ ET EMC /P <> BDC BT /FAAACH 11 Tf 1 0 0 -1 24.75 11.83899975 Tm [<003400350036000C000D>] TJ ET EMC /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 79.75 11.83899975 Tm [(\(20)] TJ ET EMC /P <> BDC BT 1 0 0 -1 96.25 11.83899975 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 107.25 11.83899975 Tm [(TBS,pH7.5\))] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 162.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 48.29999924 cm q 0.25 0.5 m 369.5 0.5 l 369.5 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(Tris)] TJ ET EMC /P <> BDC BT /FAAACH 11 Tf 1 0 0 -1 24.75 11.83899975 Tm [<003400350036000C000D>] TJ ET EMC /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 79.75 11.83899975 Tm [(\(20)] TJ ET EMC /P <> BDC BT 1 0 0 -1 96.25 11.83899975 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 107.25 11.83899975 Tm [(TBS,pH7.4\))] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 162.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 64.40000153 cm q 0.25 0.5 m 369.5 0.5 l 369.5 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(Tris)] TJ ET EMC /P <> BDC BT /FAAACH 11 Tf 1 0 0 -1 24.75 11.83899975 Tm [<003400350036000C000D>] TJ ET EMC /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 79.75 11.83899975 Tm [(\(10)] TJ ET EMC /P <> BDC BT 1 0 0 -1 96.25 11.83899975 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 107.25 11.83899975 Tm [(TBS,pH8.0\))] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 162.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 80.5 cm q 0.25 0.5 m 369.5 0.5 l 369.5 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 0 11.83899975 Tm 0 Tr [(Tris)] TJ ET EMC /P <> BDC BT /FAAACH 11 Tf 1 0 0 -1 24.75 11.83899975 Tm [<003400350036000C000D>] TJ ET EMC /P <> BDC BT /FAAACE 11 Tf 1 0 0 -1 79.75 11.83899975 Tm [(\(10)] TJ ET EMC /P <> BDC BT 1 0 0 -1 96.25 11.83899975 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 107.25 11.83899975 Tm [(TBS,pH7.5\))] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 162.25 11.65499973 Tm /GS4 gs 0 g [( )] TJ ET EMC Q Q Q q /GS2 gs 0 G 0.5 w 0 0.5 m 0 96.59999847 l S Q q /GS2 gs 0 G 0.5 w 369.75 0.5 m 369.75 96.59999847 l S Q q /GS2 gs 0 G 0.5 w -0.25 0.25 m 370 0.25 l S Q q /GS2 gs 0 G 0.5 w 0.25 16.35000038 m 369.5 16.35000038 l S Q q /GS2 gs 0 G 0.5 w 0.25 32.45000076 m 369.5 32.45000076 l S Q q /GS2 gs 0 G 0.5 w 0.25 48.55000305 m 369.5 48.55000305 l S Q q /GS2 gs 0 G 0.5 w 0.25 64.65000153 m 369.5 64.65000153 l S Q q /GS2 gs 0 G 0.5 w 0.25 80.75 m 369.5 80.75 l S Q q /GS2 gs 0 G 0.5 w -0.25 96.84999847 m 370 96.84999847 l S Q Q q 1 0 0 1 0 533.90002441 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 549.5 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 565.09997559 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 580.70001221 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 596.29998779 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 Tr /GS4 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 611.90002441 cm Q Q q 1 0 0 1 90.55000305 342.84399414 cm q 0.25 0.5 m 151.75 0.5 l 151.75 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACH 9 Tf 1 0 0 -1 52.59999847 15.00500011 Tm 0 g [<001C001D00B70037>] TJ ET EMC /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 88.59999847 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 152 0 cm 0.25 0.5 m 42.65000153 0.5 l 42.65000153 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACH 9 Tf 1 0 0 -1 7.05000019 15.00500011 Tm 0 g [<003A00B8>] TJ ET EMC /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 25.04999924 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 194.8999939 0 cm 0.25 0.5 m 59.84999847 0.5 l 59.84999847 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACH 9 Tf 1 0 0 -1 11.14999962 15.00500011 Tm 0 g [<00180019001A>] TJ ET EMC /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 38.15000153 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 255 0 cm 0.25 0.5 m 63.20000076 0.5 l 63.20000076 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACH 9 Tf 1 0 0 -1 12.82499981 15.00500011 Tm 0 g [<00B900BA00BB>] TJ ET EMC /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 39.82500076 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 318.45001221 0 cm 0.25 0.5 m 53.15000153 0.5 l 53.15000153 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACH 9 Tf 1 0 0 -1 3.29999995 15.00500011 Tm 0 g [<00BC00BD00BE00BF>] TJ ET EMC /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 39.29999924 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 23.89999962 cm q 0.25 0.5 m 151.75 0.5 l 151.75 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 0.85000002 15.00500011 Tm 0 g [(Tween20/TBS)] TJ ET EMC /P <> BDC BT 1 0 0 -1 50.34999847 15.00500011 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACH 9 Tf 1 0 0 -1 54.84999847 15.00500011 Tm [<000C000D>] TJ ET EMC /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 72.84999847 15.00500011 Tm [(\(1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 81.84999847 15.00500011 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 90.84999847 15.00500011 Tm [(TBST,pH7.4\))] TJ ET EMC /P <> BDC BT 1 0 0 -1 140.3500061 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 152 0 cm 0.25 0.5 m 42.65000153 0.5 l 42.65000153 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 4.80000019 15.00500011 Tm 0 g [(500ml)] TJ ET EMC /P <> BDC BT 1 0 0 -1 27.29999924 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 194.8999939 0 cm 0.25 0.5 m 59.84999847 0.5 l 59.84999847 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 16.77499962 15.00500011 Tm 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACH 9 Tf 1 0 0 -1 23.52499962 15.00500011 Tm [<00C0>] TJ ET EMC /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 32.52500153 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 255 0 cm 0.25 0.5 m 63.20000076 0.5 l 63.20000076 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 11.69999981 15.00500011 Tm 0 g [(12)] TJ ET EMC /P <> BDC BT /FAAACH 9 Tf 1 0 0 -1 22.95000076 15.00500011 Tm [<00C100C2>] TJ ET EMC /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 40.95000076 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 318.45001221 0 cm 0.25 0.5 m 53.15000153 0.5 l 53.15000153 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 16.04999924 15.55500031 Tm 0 g [(RT)] TJ ET EMC /P <> BDC BT /FAAACE 9 Tf 1 0 0 -1 26.54999924 15.55500031 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0 G 0.5 w 0 0.5 m 0 47.79999924 l S Q q 0 G 0.5 w 152 0.5 m 152 23.89999962 l S Q q 0 G 0.5 w 152 24.39999962 m 152 47.79999924 l S Q q 0 G 0.5 w 194.8999939 0.5 m 194.8999939 23.89999962 l S Q q 0 G 0.5 w 194.8999939 24.39999962 m 194.8999939 47.79999924 l S Q q 0 G 0.5 w 255 0.5 m 255 23.89999962 l S Q q 0 G 0.5 w 255 24.39999962 m 255 47.79999924 l S Q q 0 G 0.5 w 318.45001221 0.5 m 318.45001221 23.89999962 l S Q q 0 G 0.5 w 318.45001221 24.39999962 m 318.45001221 47.79999924 l S Q q 0 G 0.5 w 371.8500061 0.5 m 371.8500061 47.79999924 l S Q q 0 G 0.5 w -0.25 0.25 m 372.1000061 0.25 l S Q q 0 G 0.5 w 0.25 24.14999962 m 371.6000061 24.14999962 l S Q q 0 G 0.5 w -0.25 48.04999924 m 372.1000061 48.04999924 l S Q Q 1 0 0 -1 0 841.90002441 cm endstream endobj 6 0 obj <>stream ÿ??ºÝºÝÈÈÔÚÏßÊÓÆµÃâ·Ñ <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| 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