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8.40200043 Tm /GS2 gs 0 g [(  )] TJ /FAAABD 9 Tf [-500( )] TJ /GS1 gs 0 g [-250( )] TJ /FAAAAI 9 Tf 1 0 0 -1 381.29998779 8.40200043 Tm [( )] TJ ET Q q 1 0 0 1 0 83.82099915 cm Q Q EMC /Artifact <</Attached [/Bottom ] /BBox [90 49.60003662 505.29998779 59.94903564] /Type /Pagination >> BDC q 1 0 0 1 90 781.95098877 cm -90 0 m 505.29998779 0 l 505.29998779 10.34899998 l -90 10.34899998 l h W n Q EMC q 1 0 0 1 90 53.89899826 cm q 1 0 0 1 225 -0.00000076 cm q 188.3614502 0 0 -41.42535019 0 41.42535019 cm /X2 Do Q Q Q q 1 0 0 1 90 137.72000122 cm /P <</MCID 0 >> BDC BT /FAAABD 16 Tf 1 0 0 -1 33.98899841 21.47500038 Tm 0 g [(Tris-)] TJ ET EMC /P <</Lang (zh-CN) /MCID 1 >> BDC BT /FAAABH 16 Tf 1 0 0 -1 67.76200104 21.47500038 Tm 2 Tr 0.45714286 w 0 G [<0003>-4<0004>-4<0005>-4<0006>-4<0007>-4<0008>-3<0009>] TJ ET EMC /P <</MCID 2 >> BDC BT /FAAABD 16 Tf 1 0 0 -1 180.21000671 21.47500038 Tm 0 Tr [(\(10)] TJ ET EMC /P <</Lang (zh-CN) /MCID 3 >> BDC BT /FAAACB 16 Tf 1 0 0 -1 201.53799438 21.47500038 Tm 2 Tr [(?] TJ ET EMC /P <</MCID 4 >> BDC BT /FAAABD 16 Tf 1 0 0 -1 217.602005 21.47500038 Tm 0 Tr [(TPE,RNase)] TJ ET EMC /P <</MCID 5 >> BDC BT 1 0 0 -1 297.15701294 21.47500038 Tm [( )] TJ ET EMC /P <</MCID 6 >> BDC BT 1 0 0 -1 301.15701294 21.47500038 Tm [(free\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 7 >> BDC BT /FAAABH 16 Tf 1 0 0 -1 333.11801147 21.47500038 Tm 2 Tr [<000B>-4<000C>-4<000D>] TJ ET EMC /P <</MCID 8 >> BDC BT /FAAABD 16 Tf 1 0 0 -1 381.30999756 21.47500038 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC q 1 0 0 1 0 31.20000076 cm /P <</MCID 9 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 207.6499939 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 46.79999924 cm /P <</Lang (zh-CN) /MCID 10 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 102.23000336 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<000E>-4<000F>-4<0010>-4<0011>-4<0012>-4<0013>-3<0014>-4<0015>-4<0016>-4<0017>-4<0018>-4<0019>-4<001A>-4<001B>-4<0018>-4<001C>-3<001D>-4<001E>-4<001F>-4<0020>] TJ ET EMC /P <</MCID 11 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 313.07000732 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 62.40000153 cm /P <</MCID 12 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 78 cm /P <</Lang (zh-CN) /MCID 13 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<000F>-4<0010>-4<0021>-4<0022>-4<0023>] TJ ET EMC /P <</MCID 14 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 93.59999847 cm /P <</MCID 15 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris-)] TJ ET EMC /P <</Lang (zh-CN) /MCID 16 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 20.40999985 11.65499973 Tm [<000300040005000600070008000900210024>] TJ ET EMC /P <</MCID 17 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 114.91000366 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 18 >> BDC BT 1 0 0 -1 120.16000366 11.65499973 Tm [(TPE)] TJ ET EMC /P <</Lang (zh-CN) /MCID 19 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 138.82699585 11.65499973 Tm [<0019>] TJ ET EMC /P <</MCID 20 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 147.57000732 11.65499973 Tm [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 21 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 152.82000732 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 22 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 163.32000732 11.65499973 Tm [(TPE)] TJ ET EMC /P <</MCID 23 >> BDC BT 1 0 0 -1 181.98699951 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 24 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 187.23699951 11.65499973 Tm [<002500260009002700280029>] TJ ET EMC /P <</MCID 25 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 250.23699951 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 26 >> BDC BT 1 0 0 -1 255.48699951 11.65499973 Tm [(90mM)] TJ ET EMC /P <</MCID 27 >> BDC BT 1 0 0 -1 283.48999023 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 28 >> BDC BT 1 0 0 -1 286.11499023 11.65499973 Tm [(Tris-)] TJ ET EMC /P <</Lang (zh-CN) /MCID 29 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 306.5249939 11.65499973 Tm [<00030004>] TJ ET EMC /P <</Lang (zh-CN) /MCID 30 >> BDC BT 1 0 0 -1 327.5249939 11.65499973 Tm [<002A>] TJ ET EMC /P <</MCID 31 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 336.26800537 11.65499973 Tm [(2mM)] TJ ET EMC /P <</MCID 32 >> BDC BT 1 0 0 -1 359.02099609 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 33 >> BDC BT 1 0 0 -1 361.64599609 11.65499973 Tm [(EDTA)] TJ ET EMC /P <</Lang (zh-CN) /MCID 34 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 389.63900757 11.65499973 Tm [<0019>] TJ ET EMC /P <</MCID 35 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 400.13900757 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 36 >> BDC BT 1 0 0 -1 405.38900757 11.65499973 Tm [(Tris-)] TJ ET EMC /P <</Lang (zh-CN) /MCID 37 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 0 27.25500107 Tm [<0003>-11<0004>-10<0005>-11<0006>-11<0007>-10<0008>-11<0009>-11<002B>] TJ ET EMC /P <</MCID 38 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 84.89600372 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 39 >> BDC BT 1 0 0 -1 90.25800323 27.25500107 Tm [(10)] TJ ET EMC /P <</MCID 40 >> BDC BT 1 0 0 -1 100.75800323 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 41 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 106.12000275 27.25500107 Tm [<002C>-11<002D>-10<002E>-11<002F>] TJ ET EMC /P <</MCID 42 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 148.56799316 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 43 >> BDC BT 1 0 0 -1 153.92999268 27.25500107 Tm [(TPE)] TJ ET EMC /P <</Lang (zh-CN) /MCID 44 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 172.70899963 27.25500107 Tm [<0019>-11<0030>-10<0031>-11<0032>] TJ ET EMC /P <</MCID 45 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 215.15699768 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 46 >> BDC BT 1 0 0 -1 220.51899719 27.25500107 Tm [(900mM)] TJ ET EMC /P <</MCID 47 >> BDC BT 1 0 0 -1 253.77200317 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 48 >> BDC BT 1 0 0 -1 256.50900269 27.25500107 Tm [(Tris-)] TJ ET EMC /P <</Lang (zh-CN) /MCID 49 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 277.03100586 27.25500107 Tm [<0033>-11<0004>-10<0019>] TJ ET EMC /P <</MCID 50 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 308.86700439 27.25500107 Tm [(20mM)] TJ ET EMC /P <</MCID 51 >> BDC BT 1 0 0 -1 336.86999512 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 52 >> BDC BT 1 0 0 -1 339.60699463 27.25500107 Tm [(EDTA)] TJ ET EMC /P <</MCID 53 >> BDC BT 1 0 0 -1 367.6000061 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 54 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 372.96200562 27.25500107 Tm [<0034>-11<0035>-10<0036>-11<0019>] TJ ET EMC /P <</Lang (zh-CN) /MCID 55 >> BDC BT 1 0 0 -1 0 42.85499954 Tm [<0037>] TJ ET EMC /P <</MCID 56 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 10.5 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 57 >> BDC BT 1 0 0 -1 15.75 42.85499954 Tm [(DEPC)] TJ ET EMC /P <</MCID 58 >> BDC BT 1 0 0 -1 42.59000015 42.85499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 59 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 47.84000015 42.85499954 Tm [<003800390019003A>] TJ ET EMC /P <</MCID 60 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 89.83999634 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 61 >> BDC BT 1 0 0 -1 95.08999634 42.85499954 Tm [(RNase)] TJ ET EMC /P <</Lang (zh-CN) /MCID 62 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 123.08300018 42.85499954 Tm [<001900170018003B003C0018>] TJ ET EMC /P <</MCID 63 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 186.08299255 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 64 >> BDC BT 1 0 0 -1 191.33299255 42.85499954 Tm [(DEPC)] TJ ET EMC /P <</MCID 65 >> BDC BT 1 0 0 -1 218.17300415 42.85499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 66 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 223.42300415 42.85499954 Tm [<00380039003D003E003F0040>] TJ ET EMC /P <</MCID 67 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 286.42300415 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 68 >> BDC BT 1 0 0 -1 291.67300415 42.85499954 Tm [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 69 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 296.92300415 42.85499954 Tm [(?] TJ ET EMC /P <</MCID 70 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 307.42300415 42.85499954 Tm [(TPE)] TJ ET EMC /P <</MCID 71 >> BDC BT 1 0 0 -1 326.08999634 42.85499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 72 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 331.33999634 42.85499954 Tm [<00410017001800420043004400450011>] TJ ET EMC /P <</Lang (zh-CN) /MCID 73 >> BDC BT 1 0 0 -1 0 58.45499802 Tm [<0018001C00460015004700480019001A00490018001C001D001E001F0020004A004B004C0018004D0042>] TJ ET EMC /P <</MCID 74 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 220.5 58.45499802 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 156 cm /P <</MCID 75 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 171.6000061 cm /P <</Lang (zh-CN) /MCID 76 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<000F>-4<0010>-4<0035>-4<0036>-4<0023>] TJ ET EMC /P <</MCID 77 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 187.19999695 cm /P <</MCID 78 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 -10 202.80000305 cm q 0.25 0.5 m 178.6000061 0.5 l 178.6000061 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 79 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 65.95300293 16.22999954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<000F>-4<0010>-4<004E>-4<0024>] TJ ET EMC /P <</MCID 80 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 102.09700012 16.22999954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 178.8500061 0 cm 0.25 0.5 m 39.5 0.5 l 39.5 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 81 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 5.43900013 16.22999954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<004F>-4<0050>] TJ ET EMC /P <</MCID 82 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 23.51099968 16.22999954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 218.6000061 0 cm 0.25 0.5 m 50 0.5 l 50 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 83 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 1.653 16.22999954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0051>-4<0052>-4<0053>-4<0054>] TJ ET EMC /P <</MCID 84 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 37.79700089 16.22999954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 268.8500061 0 cm 0.25 0.5 m 38.75 0.5 l 38.75 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 85 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 0.546 16.22999954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<000B>-4<000C>-4<000D>] TJ ET EMC /P <</MCID 86 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 27.65399933 16.22999954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 307.8500061 0 cm 0.25 0.5 m 42.5 0.5 l 42.5 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 87 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 2.421 16.22999954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0029>-4<0055>-4<0056>] TJ ET EMC /P <</MCID 88 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 29.52899933 16.22999954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 26.35000038 cm q 0.25 0.5 m 178.6000061 0.5 l 178.6000061 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 89 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 2.54099989 16.22999954 Tm /GS2 gs 0 g [(Tris-)] TJ ET EMC /P <</Lang (zh-CN) /MCID 90 >> BDC BT /FAAACD 9 Tf 1 0 0 -1 20.0359993 16.22999954 Tm [<0003000400050006000700080009>] TJ ET EMC /P <</MCID 91 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 83.03600311 16.22999954 Tm [(\(10)] TJ ET EMC /P <</Lang (zh-CN) /MCID 92 >> BDC BT /FAAACH 9 Tf 1 0 0 -1 95.03299713 16.22999954 Tm [(?] TJ ET EMC /P <</MCID 93 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 104.03299713 16.22999954 Tm [(TPE,RNase)] TJ ET EMC /P <</MCID 94 >> BDC BT 1 0 0 -1 146.27799988 16.22999954 Tm [( )] TJ ET EMC /P <</MCID 95 >> BDC BT 1 0 0 -1 148.52799988 16.22999954 Tm [(free\))] TJ ET EMC /P <</MCID 96 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 165.50799561 16.22999954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 178.8500061 0 cm 0.25 0.5 m 39.5 0.5 l 39.5 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 97 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 2.97399998 16.15200043 Tm /GS2 gs 0 g [(500ml)] TJ ET EMC /P <</MCID 98 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 25.97500038 16.15200043 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 218.6000061 0 cm 0.25 0.5 m 50 0.5 l 50 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 99 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 13.97500038 16.15200043 Tm /GS2 gs 0 g [(RT)] TJ ET EMC /P <</MCID 100 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 25.47500038 16.15200043 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 268.8500061 0 cm 0.25 0.5 m 38.75 0.5 l 38.75 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 101 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 6.2249999 16.22999954 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 102 >> BDC BT /FAAACD 9 Tf 1 0 0 -1 12.97500038 16.22999954 Tm [<0057>] TJ ET EMC /P <</MCID 103 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 21.97500038 16.22999954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 307.8500061 0 cm 0.25 0.5 m 42.5 0.5 l 42.5 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 104 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 8.10000038 16.22999954 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 105 >> BDC BT /FAAACD 9 Tf 1 0 0 -1 14.85000038 16.22999954 Tm [<0058>] TJ ET EMC /P <</MCID 106 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 23.85000038 16.22999954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 52.70000076 cm q 0.25 0.5 m 178.6000061 0.5 l 178.6000061 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 107 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 43.91400146 16.22999954 Tm /GS2 gs 0 g [(10)] TJ ET EMC /P <</Lang (zh-CN) /MCID 108 >> BDC BT /FAAACH 9 Tf 1 0 0 -1 52.91400146 16.22999954 Tm [(?] TJ ET EMC /P <</MCID 109 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 61.91400146 16.22999954 Tm [(TPE\(RNase)] TJ ET EMC /P <</MCID 110 >> BDC BT 1 0 0 -1 104.90599823 16.22999954 Tm [( )] TJ ET EMC /P <</MCID 111 >> BDC BT 1 0 0 -1 107.15599823 16.22999954 Tm [(free\))] TJ ET EMC /P <</MCID 112 >> BDC BT 1 0 0 -1 124.13600159 16.22999954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 178.8500061 0 cm 0.25 0.5 m 39.5 0.5 l 39.5 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 113 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 2.97399998 16.15200043 Tm /GS2 gs 0 g [(500ml)] TJ ET EMC /P <</MCID 114 >> BDC BT 1 0 0 -1 25.97500038 16.15200043 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 218.6000061 0 cm 0.25 0.5 m 50 0.5 l 50 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 115 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 13.97500038 16.15200043 Tm /GS2 gs 0 g [(RT)] TJ ET EMC /P <</MCID 116 >> BDC BT 1 0 0 -1 25.47500038 16.15200043 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 268.8500061 0 cm 0.25 0.5 m 38.75 0.5 l 38.75 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 117 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 6.2249999 16.22999954 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 118 >> BDC BT /FAAACD 9 Tf 1 0 0 -1 12.97500038 16.22999954 Tm [<0057>] TJ ET EMC /P <</MCID 119 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 21.97500038 16.22999954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 307.8500061 0 cm 0.25 0.5 m 42.5 0.5 l 42.5 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 120 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 8.10000038 16.22999954 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 121 >> BDC BT /FAAACD 9 Tf 1 0 0 -1 14.85000038 16.22999954 Tm [<0058>] TJ ET EMC /P <</MCID 122 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 23.85000038 16.22999954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0.5 w 0 0.5 m 0 79.05000305 l S Q q 0.5 w 178.8500061 0.5 m 178.8500061 26.35000038 l S Q q 0.5 w 178.8500061 26.85000038 m 178.8500061 52.70000076 l S Q q 0.5 w 178.8500061 53.20000076 m 178.8500061 79.05000305 l S Q q 0.5 w 218.6000061 0.5 m 218.6000061 26.35000038 l S Q q 0.5 w 218.6000061 26.85000038 m 218.6000061 52.70000076 l S Q q 0.5 w 218.6000061 53.20000076 m 218.6000061 79.05000305 l S Q q 0.5 w 268.8500061 0.5 m 268.8500061 26.35000038 l S Q q 0.5 w 268.8500061 26.85000038 m 268.8500061 52.70000076 l S Q q 0.5 w 268.8500061 53.20000076 m 268.8500061 79.05000305 l S Q q 0.5 w 307.8500061 0.5 m 307.8500061 26.35000038 l S Q q 0.5 w 307.8500061 26.85000038 m 307.8500061 52.70000076 l S Q q 0.5 w 307.8500061 53.20000076 m 307.8500061 79.05000305 l S Q q 0.5 w 350.6000061 0.5 m 350.6000061 79.05000305 l S Q q 0.5 w -0.25 0.25 m 350.8500061 0.25 l S Q q 0.5 w 0.25 26.60000038 m 350.3500061 26.60000038 l S Q q 0.5 w 0.25 52.95000076 m 350.3500061 52.95000076 l S Q q 0.5 w -0.25 79.30000305 m 350.8500061 79.30000305 l S Q Q q 1 0 0 1 0 282.3500061 cm /P <</MCID 123 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 297.95001221 cm /P <</Lang (zh-CN) /MCID 124 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<0059>-4<0026>-4<005A>-4<005B>] TJ ET EMC /P <</MCID 125 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 42.16799927 11.65499973 Tm 0 Tr [(\()] TJ ET EMC /P <</Lang (zh-CN) /MCID 126 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 45.66500092 11.65499973 Tm 2 Tr [<0011>-4<0012>-4<005C>-4<005D>] TJ ET EMC /P <</MCID 127 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 87.83300018 11.65499973 Tm 0 Tr [(\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 128 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 91.33000183 11.65499973 Tm 2 Tr [<0023>] TJ ET EMC /P <</MCID 129 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 101.87200165 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 313.54998779 cm /P <</MCID 130 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 131 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<002A0018>] TJ ET EMC /P <</MCID 132 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 26.25 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 133 >> BDC BT 1 0 0 -1 31.5 11.65499973 Tm [(DEPC)] TJ ET EMC /P <</MCID 134 >> BDC BT 1 0 0 -1 58.34000015 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 135 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 63.59000015 11.65499973 Tm [<00380039003D005E>] TJ ET EMC /P <</MCID 136 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 105.58999634 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 137 >> BDC BT 1 0 0 -1 110.83999634 11.65499973 Tm [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 138 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 116.08999634 11.65499973 Tm [<000A0041001700180042>] TJ ET EMC /P <</MCID 139 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 168.58999634 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 329.1499939 cm /P <</MCID 140 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 344.75 cm /P <</Lang (zh-CN) /MCID 141 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [<005F0060006100620023>] TJ ET EMC /P <</MCID 142 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 52.5 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 360.3500061 cm /P <</MCID 143 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 144 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<002A005F006000630064006500040066006700680042>] TJ ET EMC /P <</MCID 145 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 120.75 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 375.95001221 cm /P <</MCID 146 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(2)] TJ ET EMC /P <</Lang (zh-CN) /MCID 147 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<002A00400069006A002F006B006C006D006E006F00190070007100720073007400750076007700780079007A007B005900260042>] TJ ET EMC /P <</MCID 148 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 278.25 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 391.54998779 cm /P <</MCID 149 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 407.1499939 cm /P <</Lang (zh-CN) /MCID 150 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<007C>-4<007D>-4<000F>-4<0010>-4<0023>] TJ ET EMC /P <</MCID 151 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 422.75 cm /P <</MCID 152 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 -5.4000001 438.3500061 cm q 0.25 0.5 m 297.75 0.5 l 297.75 24.45000076 l 0.25 24.45000076 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 153 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris-Tricine)] TJ ET EMC /P <</Lang (zh-CN) /MCID 154 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 65.625 11.65499973 Tm [<00860087000700080009>] TJ ET EMC /P <</MCID 155 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 118.125 11.65499973 Tm [(\(5)] TJ ET EMC /P <</Lang (zh-CN) /MCID 156 >> BDC BT 1 0 0 -1 128.625 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 157 >> BDC BT 1 0 0 -1 139.125 11.65499973 Tm [(,pH8.9\))] TJ ET EMC /P <</MCID 158 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 175.875 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 24.45000076 cm q 0.25 0.5 m 297.75 0.5 l 297.75 24.45000076 l 0.25 24.45000076 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 159 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris-MOPS-SDS)] TJ ET EMC /P <</Lang (zh-CN) /MCID 160 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 70.875 11.65499973 Tm [<00050006000700080009>] TJ ET EMC /P <</MCID 161 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 123.375 11.65499973 Tm [(\(20)] TJ ET EMC /P <</Lang (zh-CN) /MCID 162 >> BDC BT 1 0 0 -1 139.125 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 163 >> BDC BT 1 0 0 -1 149.625 11.65499973 Tm [(,pH8.3\))] TJ ET EMC /P <</MCID 164 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 186.375 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 48.90000153 cm q 0.25 0.5 m 297.75 0.5 l 297.75 24.45000076 l 0.25 24.45000076 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 165 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris-MOPS-SDS)] TJ ET EMC /P <</Lang (zh-CN) /MCID 166 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 70.875 11.65499973 Tm [<0005000600990045>] TJ ET EMC /P <</MCID 167 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 112.875 11.65499973 Tm [(\(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 168 >> BDC BT 1 0 0 -1 123.375 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 169 >> BDC BT 1 0 0 -1 133.875 11.65499973 Tm [(\))] TJ ET EMC /P <</MCID 170 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 139.125 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 73.34999847 cm q 0.25 0.5 m 297.75 0.5 l 297.75 24.45000076 l 0.25 24.45000076 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 171 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris-HCl)] TJ ET EMC /P <</Lang (zh-CN) /MCID 172 >> BDC BT /FAAACD 10.5 Tf 1 0 0 -1 44.625 11.65499973 Tm [<000700080009>] TJ ET EMC /P <</MCID 173 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 76.125 11.65499973 Tm [(\(1mol_L,pH6.8\))] TJ ET EMC /P <</MCID 174 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 149.625 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0.5 w 0 0.5 m 0 97.80000305 l S Q q 0.5 w 298 0.5 m 298 97.80000305 l S Q q 0.5 w -0.25 0.25 m 298.25 0.25 l S Q q 0.5 w 0.25 24.70000076 m 297.75 24.70000076 l S Q q 0.5 w 0.25 49.15000153 m 297.75 49.15000153 l S Q q 0.5 w 0.25 73.6000061 m 297.75 73.6000061 l S Q q 0.5 w -0.25 98.05000305 m 298.25 98.05000305 l S Q Q q 1 0 0 1 0 536.65002441 cm /P <</MCID 175 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 552.25 cm Q Q 1 0 0 -1 0 841.90002441 cm 
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