%PDF-1.7 %ÈÈÈÈÈÈ?1 0 obj <> endobj 2 0 obj <>/Metadata 93 0 R>> endobj 3 0 obj <> endobj 4 0 obj <>/ExtGState<>/XObject<>>>/Group<>/StructParents 0/Tabs/S>> endobj 5 0 obj <>stream 1 0 0 -1 0 841.90002441 cm q 1 0 0 1 65.65000153 94.18599701 cm q 474.8500061 0 0 -20.80000114 0 20.80000114 cm /X1 Do Q Q /Artifact <> BDC q 1 0 0 1 90 42.54999924 cm -90 0 m 505.29998779 0 l 505.29998779 87.94400024 l -90 87.94400024 l h W n BT /FAAAAI 9 Tf 1 0 0 -1 0 9.56900024 Tm 0 g [( )-1( )-1( )-1( )] TJ /GS1 gs 0 g [-11231( )] TJ ET q 1 0 0 1 0 11.98600006 cm BT 1 0 0 -1 0 42 Tm /GS2 gs 0 g [( )-1( )-1( )-1( )-1( )] TJ 1 0 0 -1 414 42 Tm /GS1 gs 0 g [( )] TJ ET Q q 1 0 0 1 0 53.98600006 cm BT 1 0 0 -1 0 8.56900024 Tm /GS2 gs 0 g [( )-1( )-1( )] TJ /GS1 gs 0 g [-9040( )] TJ ET Q q 1 0 0 1 0 64.97200012 cm BT 1 0 0 -1 0 8.56900024 Tm [( )] TJ ET Q q 1 0 0 1 0 75.95800018 cm BT 1 0 0 -1 0 8.56900024 Tm /GS2 gs 0 g [( )] TJ /FAAABD 9 Tf [-548( )] TJ ET Q Q EMC /Artifact <> BDC q 1 0 0 1 90 781.31402588 cm -90 0 m 505.29998779 0 l 505.29998779 10.98600006 l -90 10.98600006 l h W n Q EMC q 1 0 0 1 90 54.5359993 cm q 1 0 0 1 225 0 cm q 188.3500061 0 0 -41.45000076 0 41.45000076 cm /X2 Do Q Q Q q 1 0 0 1 90 130.4940033 cm q 1 0 0 1 22 0 cm /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 94.37200165 23.67700005 Tm 2 Tr 0 g 0.62857145 w 0 G [(T)-4(7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 116.54799652 23.67700005 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 127.63600159 23.67700005 Tm [(R)-4(N)-4(A)] TJ ET EMC /P <> BDC BT /FAAABJ 22 Tf 1 0 0 -1 166.3999939 23.67700005 Tm [<0007>-4<0008>-4<0009>-4<000A>-4<000B>-4<000C>] TJ ET EMC /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 298.92800903 23.67700005 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 31.20000076 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 207.6499939 11.65499973 Tm 1 Tr 0.30000001 w /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 46.79999924 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 89.10500336 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [( )-4( )-4( )-4( )-4( )] TJ ET EMC /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 115.35500336 11.65499973 Tm [<000D>-4<000E>-4<000F>-4<0010>-4<0011>-4<0012>-3<0013>-4<0014>-4<0015>-4<0016>-4<0017>-4<0018>-4<0019>-4<001A>-4<0017>-4<001B>-3<001C>-4<001D>-4<001E>-4<001F>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 326.19500732 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 62.40000153 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 78 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<0020>-4<0021>-4<0022>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 31.62599945 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 93.59999847 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 10.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 15.75 11.65499973 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 31.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 36.75 11.65499973 Tm [(Polymerase)] TJ ET EMC /P <> BDC BT 1 0 0 -1 89.25 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 94.5 11.65499973 Tm [<002C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 105 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 110.25 11.65499973 Tm [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 120.75 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 126 11.65499973 Tm [<002D>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 136.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 141.75 11.65499973 Tm [<002E>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 152.25 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 157.5 11.65499973 Tm [<0012>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 168 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 173.25 11.65499973 Tm [<002F>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 183.75 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 189 11.65499973 Tm [<0030>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 199.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 204.75 11.65499973 Tm [<0031>] TJ ET EMC /P <> BDC BT 1 0 0 -1 210.2749939 11.65499973 Tm [<0032>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 220.7749939 11.65499973 Tm [(TAATACGACT)] TJ ET EMC /P <> BDC BT 1 0 0 -1 273.2749939 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 278.5249939 11.65499973 Tm [(CACTATAG)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 320.5249939 11.65499973 Tm [<0035>] TJ ET EMC /P <> BDC BT 1 0 0 -1 326.04998779 11.65499973 Tm [<0036003700380039003A003B003C003D>] TJ ET EMC /P <> BDC BT 1 0 0 -1 410.04998779 11.65499973 Tm [<0018>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [<003E003F0040004100420043002F003000310018>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 105 27.25500107 Tm [(DNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 120.75 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 126 27.25500107 Tm [<0042004300450046001200130008004700480049004A004B004C0049004A>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 286.125 27.25500107 Tm [(RNA)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 301.875 27.25500107 Tm [<004D004E004A004F003D005000510052004B>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 396.375 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 401.625 27.25500107 Tm [(5)] TJ ET EMC /P <> BDC BT 1 0 0 -1 406.875 27.25500107 Tm [(?] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 0 42.85499954 Tm [<0055005600510052>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 42 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 44.625 42.85499954 Tm [(DNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 60.375 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 63 42.85499954 Tm [<0057003F00420043>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 105 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 107.625 42.85499954 Tm [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 118.125 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 120.75 42.85499954 Tm [(RNAPolymerase)] TJ ET EMC /P <> BDC BT 1 0 0 -1 189 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 191.625 42.85499954 Tm [<003A0058005900450046>] TJ ET EMC /P <> BDC BT 1 0 0 -1 244.125 42.85499954 Tm [<0018>] TJ ET EMC /P <> BDC BT 1 0 0 -1 252.2749939 42.85499954 Tm [<005A005B004F003D005C005D>] TJ ET EMC /P <> BDC BT 1 0 0 -1 315.2749939 42.85499954 Tm [<005E>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 323.42498779 42.85499954 Tm [(PCR)] TJ ET EMC /P <> BDC BT 1 0 0 -1 339.17498779 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 341.79998779 42.85499954 Tm [<000E00590057003F001700420012>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 58.45499802 Tm [<001300080047>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 31.5 58.45499802 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 36.75 58.45499802 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 52.5 58.45499802 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 57.75 58.45499802 Tm [<003A00450046004D>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 99.75 58.45499802 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 105 58.45499802 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 156 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 171.6000061 cm /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [<00480049004B004C0049>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 52.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 57.75 11.65499973 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 73.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 78.75 11.65499973 Tm [<004A005F0060001B00450046>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 141.75 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 147 11.65499973 Tm [(DNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 162.75 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 168 11.65499973 Tm [<006100620063>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 199.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 204.75 11.65499973 Tm [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 215.25 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 220.5 11.65499973 Tm [<002F00300031003A00640065>] TJ ET EMC /P <> BDC BT 1 0 0 -1 283.5 11.65499973 Tm [<0018>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 288.75 11.65499973 Tm [(DNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 304.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 309.75 11.65499973 Tm [<0064001B>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 330.75 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 336 11.65499973 Tm [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 346.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 351.75 11.65499973 Tm [<002F00300031003A00660067>] TJ ET EMC /P <> BDC BT 1 0 0 -1 414.75 11.65499973 Tm [<0018>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 27.25500107 Tm [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 10.5 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 15.88799953 27.25500107 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 31.63800049 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 37.02600098 27.25500107 Tm [<0007>-13<0008>-13<0009>-13<0068>-14<0069>-13<006A>-13<0056>-13<0048>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 122.12999725 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 127.51799774 27.25500107 Tm [<0049>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 138.15600586 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 143.54400635 27.25500107 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 159.29400635 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 164.68200684 27.25500107 Tm [<0018>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 175.32000732 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 180.70799255 27.25500107 Tm [<004C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 191.34599304 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 196.73399353 27.25500107 Tm [<006B>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 207.37199402 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 212.75999451 27.25500107 Tm [<006C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 223.397995 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 228.78599548 27.25500107 Tm [<0043>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 239.42399597 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 244.81199646 27.25500107 Tm [<004C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 255.44999695 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 260.8380127 27.25500107 Tm [<0049>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 271.47601318 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 276.86401367 27.25500107 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 292.61401367 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 298.00201416 27.25500107 Tm [<004A>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 308.64001465 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 314.02801514 27.25500107 Tm [<004D>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 324.66598511 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 330.0539856 27.25500107 Tm [<006D>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 340.69198608 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 346.07998657 27.25500107 Tm [<0009>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 356.71798706 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 362.10598755 27.25500107 Tm [<006E>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 372.74398804 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 378.13198853 27.25500107 Tm [<006F>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 388.76998901 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 394.1579895 27.25500107 Tm [<0070>-13<0071>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 415.43399048 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 42.85499954 Tm [(E.coli)] TJ ET EMC /P <> BDC BT 1 0 0 -1 31.5 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 36.75 42.85499954 Tm [(BL21)] TJ ET EMC /P <> BDC BT 1 0 0 -1 57.75 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 63 42.85499954 Tm [<002E007A007B007C007D001A0018007E>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 147 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 152.25 42.85499954 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 168 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 173.25 42.85499954 Tm [<000700080009005E>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 215.25 42.85499954 Tm [(DNase)] TJ ET EMC /P <> BDC BT 1 0 0 -1 241.5 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 246.75 42.85499954 Tm [<007F>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 259.875 42.85499954 Tm [(RNase)] TJ ET EMC /P <> BDC BT 1 0 0 -1 286.125 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 291.375 42.85499954 Tm [<00800081004D>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 322.875 42.85499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 218.3999939 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 273 93.30000305 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 312 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 327.6000061 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<0082>-4<003D>-4<0083>-4<0049>] TJ ET EMC /P <> BDC BT 1 0 0 -1 41.02600098 11.65499973 Tm [<0022>] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 50.42599869 11.65499973 Tm 0 Tr [<008400850086004C00870012008800660018>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 144.92599487 11.65499973 Tm [(37)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 155.42599487 11.65499973 Tm [<008A>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 165.92599487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 171.17599487 11.65499973 Tm [(1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 176.42599487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 181.67599487 11.65499973 Tm [<008B008C008D008E>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 223.67599487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 228.92599487 11.65499973 Tm [(1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 234.17599487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 239.42599487 11.65499973 Tm [(nmol)] TJ ET EMC /P <> BDC BT 1 0 0 -1 260.42599487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 265.67599487 11.65499973 Tm [<003A>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 278.80099487 11.65499973 Tm [(ATP)] TJ ET EMC /P <> BDC BT 1 0 0 -1 294.55099487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 299.80099487 11.65499973 Tm [<009000910092001900930059009400950096003A0009>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [<0097008300490043009800990082003D009A0064004D>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 115.5 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 120.75 27.25500107 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 358.79998779 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 374.3999939 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(1X)] TJ ET EMC /P <> BDC BT 1 0 0 -1 10.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 13.36999989 11.65499973 Tm [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 23.87000084 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 26.73999977 11.65499973 Tm [(Transcription)] TJ ET EMC /P <> BDC BT 1 0 0 -1 94.98999786 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 97.86000061 11.65499973 Tm [(Buffer)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 129.36000061 11.65499973 Tm [<0022>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 139.86000061 11.65499973 Tm [(40)] TJ ET EMC /P <> BDC BT 1 0 0 -1 150.36000061 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 153.22999573 11.65499973 Tm [(mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 163.72999573 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 166.6000061 11.65499973 Tm [(Tris)] TJ ET EMC /P <> BDC BT 1 0 0 -1 187.6000061 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 190.47000122 11.65499973 Tm [(\(pH)] TJ ET EMC /P <> BDC BT 1 0 0 -1 206.22000122 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 209.08999634 11.65499973 Tm [(7.8\),)] TJ ET EMC /P <> BDC BT 1 0 0 -1 235.33999634 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 238.21000671 11.65499973 Tm [(10)] TJ ET EMC /P <> BDC BT 1 0 0 -1 248.71000671 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 251.58000183 11.65499973 Tm [(mMNaCl,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 288.32998657 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 291.20001221 11.65499973 Tm [(6mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 306.95001221 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 309.82000732 11.65499973 Tm [(MgCl2,2)] TJ ET EMC /P <> BDC BT 1 0 0 -1 346.57000732 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 349.44000244 11.65499973 Tm [(mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 359.94000244 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 362.80999756 11.65499973 Tm [(Spermidine)] TJ ET EMC /P <> BDC BT 1 0 0 -1 415.30999756 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [(HCl,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 21 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 26.25 27.25500107 Tm [(10)] TJ ET EMC /P <> BDC BT 1 0 0 -1 36.75 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 42 27.25500107 Tm [(mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 52.5 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 57.75 27.25500107 Tm [(DTT)] TJ ET EMC /P <> BDC BT 1 0 0 -1 73.5 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 78.75 27.25500107 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 405.6000061 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 421.20001221 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<0009>-4<00AC>-4<00AD>-4<00AE>-4<0022>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr [(10)] TJ ET EMC /P <> BDC BT 1 0 0 -1 63.20999908 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 66.23500061 11.65499973 Tm [(mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 76.73500061 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 79.76000214 11.65499973 Tm [(Tris-HCl,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 127.01000214 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 130.03500366 11.65499973 Tm [(0.1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 145.78500366 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 148.80999756 11.65499973 Tm [(M)] TJ ET EMC /P <> BDC BT 1 0 0 -1 154.05999756 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 157.08500671 11.65499973 Tm [(NaCl,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 183.33500671 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 186.36000061 11.65499973 Tm [(1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 191.61000061 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 194.63499451 11.65499973 Tm [(mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 205.13499451 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 208.16000366 11.65499973 Tm [(DTT,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 229.16000366 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 232.18499756 11.65499973 Tm [(0.1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 247.93499756 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 250.96000671 11.65499973 Tm [(mMEDTA,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 287.70999146 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 290.73498535 11.65499973 Tm [(50%)] TJ ET EMC /P <> BDC BT 1 0 0 -1 306.48498535 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 309.51000977 11.65499973 Tm [(Glycerol,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 356.76000977 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 359.78500366 11.65499973 Tm [(0.01%)] TJ ET EMC /P <> BDC BT 1 0 0 -1 386.03500366 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 389.05999756 11.65499973 Tm [(\(w/v\))] TJ ET EMC /P <> BDC BT 1 0 0 -1 415.30999756 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [(Triton)] TJ ET EMC /P <> BDC BT 1 0 0 -1 31.5 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 36.75 27.25500107 Tm [(X-100,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 68.25 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 73.5 27.25500107 Tm [(pH)] TJ ET EMC /P <> BDC BT 1 0 0 -1 84 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 89.25 27.25500107 Tm [(7.5)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 105 27.25500107 Tm [<004D>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 115.5 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 120.75 27.25500107 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 452.3999939 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 468 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<00AC>-4<00AD>-4<0022>] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 31.62599945 11.65499973 Tm 0 Tr [<0065001B>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 52.62599945 11.65499973 Tm [(-20)] TJ ET EMC /P <> BDC BT 1 0 0 -1 68.37599945 11.65499973 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 78.87599945 11.65499973 Tm [(C)] TJ ET EMC /P <> BDC BT 1 0 0 -1 84.12599945 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 89.37599945 11.65499973 Tm [<003F00B500AD>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 120.87599945 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 126.12599945 11.65499973 Tm [(2)] TJ ET EMC /P <> BDC BT 1 0 0 -1 131.37600708 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 136.62600708 11.65499973 Tm [<00B6001800B700B8004C00B900BA00BB004D>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 231.12600708 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 236.37600708 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 483.6000061 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 499.20001221 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<00BC>-4<00BD>-4<0082>-4<0022>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 42.16799927 11.65499973 Tm 0 Tr [(70)] TJ ET EMC /P <> BDC BT 1 0 0 -1 52.66799927 11.65499973 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 63.16799927 11.65499973 Tm [(C)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 68.41799927 11.65499973 Tm [<0018>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 78.91799927 11.65499973 Tm [(10min)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 105.16799927 11.65499973 Tm [<004D>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 115.66799927 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 120.91799927 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 514.79998779 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 530.40002441 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<00BE>-4<0042>-4<00BF>-4<00C0>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 42.16799927 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 546 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 561.59997559 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(1.)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 10.5 11.65499973 Tm [<00C100C2004C008700120088>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 73.5 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 577.20001221 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 90 348.89398193 cm /P <> BDC q 1 0 0 1 0 0.30000305 cm q 272.25 0 0 -93 0 93 cm /X3 Do Q Q EMC Q 1 0 0 -1 0 841.90002441 cm endstream endobj 6 0 obj <>stream ÿ??ºÝºÝÈÈÔÚÏßÊÓÆµÃâ·Ñ <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Îı¾Á´> <Îı¾Á´> <Îı¾Á´> <Îı¾Á´> <Îı¾Á´> <Îı¾Á´>