%PDF-1.7 %ÈÈÈÈÈÈ?1 0 obj <> endobj 2 0 obj <>/Metadata 87 0 R>> endobj 3 0 obj <> endobj 4 0 obj <>/ExtGState<>/XObject<>>>/Group<>/StructParents 0/Tabs/S>> endobj 5 0 obj <>stream 1 0 0 -1 0 841.90002441 cm q 1 0 0 1 65.65000153 94.18599701 cm q 474.8500061 0 0 -20.80000114 0 20.80000114 cm /X1 Do Q Q /Artifact <> BDC q 1 0 0 1 90 42.54999924 cm -90 0 m 505.29998779 0 l 505.29998779 87.94400024 l -90 87.94400024 l h W n BT /FAAAAI 9 Tf 1 0 0 -1 0 9.56900024 Tm 0 g [( )-1( )-1( )-1( )] TJ /GS1 gs 0 g [-11231( )] TJ ET q 1 0 0 1 0 11.98600006 cm BT 1 0 0 -1 0 42 Tm /GS2 gs 0 g [( )-1( )-1( )-1( )-1( )] TJ 1 0 0 -1 414 42 Tm /GS1 gs 0 g [( )] TJ ET Q q 1 0 0 1 0 53.98600006 cm BT 1 0 0 -1 0 8.56900024 Tm /GS2 gs 0 g [( )-1( )-1( )] TJ /GS1 gs 0 g [-9040( )] TJ ET Q q 1 0 0 1 0 64.97200012 cm BT 1 0 0 -1 0 8.56900024 Tm [( )] TJ ET Q q 1 0 0 1 0 75.95800018 cm BT 1 0 0 -1 0 8.56900024 Tm /GS2 gs 0 g [( )] TJ /FAAABD 9 Tf [-548( )] TJ ET Q Q EMC /Artifact <> BDC q 1 0 0 1 90 781.31402588 cm -90 0 m 505.29998779 0 l 505.29998779 10.98600006 l -90 10.98600006 l h W n Q EMC q 1 0 0 1 90 54.5359993 cm q 1 0 0 1 225 0 cm q 188.3500061 0 0 -41.45000076 0 41.45000076 cm /X2 Do Q Q Q q 1 0 0 1 90 130.4940033 cm q 1 0 0 1 22 0 cm /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 0 23.67700005 Tm 2 Tr 0 g 0.62857145 w 0 G [(T)-4(7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 22.1760006 23.67700005 Tm [( )] TJ ET EMC /P <> BDC BT /FAAABJ 22 Tf 1 0 0 -1 33.17599869 23.67700005 Tm [<0005>-4<0006>] TJ ET EMC /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 82.85199738 23.67700005 Tm [(R)-4(N)-4(A)] TJ ET EMC /P <> BDC BT /FAAABJ 22 Tf 1 0 0 -1 121.61599731 23.67700005 Tm [<0009>-4<000A>-4<000B>] TJ ET EMC /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 193.38000488 23.67700005 Tm [(2)-4(.)-4(0)] TJ ET EMC /P <> BDC BT /FAAABJ 22 Tf 1 0 0 -1 226.64399719 23.67700005 Tm [<000F>-4<0010>-4<0011>-4<0012>] TJ ET EMC /P <> BDC BT 1 0 0 -1 314.9960022 23.67700005 Tm [<0013>] TJ ET EMC /P <> BDC BT 1 0 0 -1 327.03601074 23.67700005 Tm [<0014>-4<0015>-4<0016>] TJ ET EMC /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 393.29998779 23.67700005 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 31.20000076 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 207.6499939 11.65499973 Tm 1 Tr 0.30000001 w /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 46.79999924 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 102.23000336 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<0017>-4<0018>-4<0019>-4<001A>-4<001B>-4<001C>-3<001D>-4<001E>-4<001F>-4<0020>-4<0021>-4<0022>-4<0023>-4<0024>-4<0021>-4<0025>-3<0026>-4<0027>-4<0028>-4<0029>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 313.07000732 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 62.40000153 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 78 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<002A>-4<002B>-4<002C>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 31.62599945 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 93.59999847 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 10.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 13.125 11.65499973 Tm [<00050006>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 34.125 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 36.75 11.65499973 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 52.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 55.125 11.65499973 Tm [<0009000A000B>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 86.625 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 89.25 11.65499973 Tm [(2.0)] TJ ET EMC /P <> BDC BT 1 0 0 -1 105 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 107.625 11.65499973 Tm [<002D002E002F00300031003200330034003500360037>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 223.125 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 225.75 11.65499973 Tm [(DNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 241.5 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 244.125 11.65499973 Tm [<0039003A003B>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 275.625 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 278.25 11.65499973 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 294 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 296.625 11.65499973 Tm [<0009000A000B>] TJ ET EMC /P <> BDC BT 1 0 0 -1 328.125 11.65499973 Tm [<0022>] TJ ET EMC /P <> BDC BT 1 0 0 -1 336.54998779 11.65499973 Tm [<003C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 347.04998779 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 349.67498779 11.65499973 Tm [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 360.17498779 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 362.79998779 11.65499973 Tm [<003D003E001C003F0040>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [<0041000F>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 21 27.25500107 Tm [(TAATACGACT)] TJ ET EMC /P <> BDC BT 1 0 0 -1 73.5 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 76.26300049 27.25500107 Tm [(CACTATAG)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 118.26300049 27.25500107 Tm [<0013004400450010001200460047003B002200480049004A004B003D003E001C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 286.26300049 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 289.02600098 27.25500107 Tm [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 299.52600098 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 302.28900146 27.25500107 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 318.03900146 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 320.80200195 27.25500107 Tm [<0009000A000B004C004D0022004E004F0050>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 42.85499954 Tm [<00510010003700520012005300540055001C001D00560057>] TJ ET EMC /P <> BDC BT 1 0 0 -1 126 42.85499954 Tm [<0058>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 131.80000305 42.85499954 Tm [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 142.30000305 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 144.92500305 42.85499954 Tm [<00050006>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 165.92500305 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 168.55000305 42.85499954 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 184.30000305 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 186.92500305 42.85499954 Tm [<0009000A000B>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 218.42500305 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 221.05000305 42.85499954 Tm [(2.0)] TJ ET EMC /P <> BDC BT 1 0 0 -1 236.80000305 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 239.42500305 42.85499954 Tm [<004F0050>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 260.42498779 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 263.04998779 42.85499954 Tm [(37-52)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 289.29998779 42.85499954 Tm [<005C005D005E0053005400550010005F0037001C001D0056>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 58.45499802 Tm [<0057>] TJ ET EMC /P <> BDC BT 1 0 0 -1 10.5 58.45499802 Tm [<0022>] TJ ET EMC /P <> BDC BT 1 0 0 -1 19.82500076 58.45499802 Tm [<00600061005200120062>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 72.32499695 58.45499802 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 74.94999695 58.45499802 Tm [(37)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 85.44999695 58.45499802 Tm [<005C>] TJ ET EMC /P <> BDC BT 1 0 0 -1 95.94999695 58.45499802 Tm [<0022>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 105.27500153 58.45499802 Tm [(50)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 115.77500153 58.45499802 Tm [<005C00630064005D005E0053>] TJ ET EMC /P <> BDC BT 1 0 0 -1 178.7749939 58.45499802 Tm [<0022>] TJ ET EMC /P <> BDC BT 1 0 0 -1 188.1000061 58.45499802 Tm [<0065006600670068>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 230.1000061 58.45499802 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 232.7250061 58.45499802 Tm [(50%)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 248.4750061 58.45499802 Tm [<006A006B0037006C003B>] TJ ET EMC /P <> BDC BT 1 0 0 -1 299.79998779 58.45499802 Tm [<000F0049004A004B0050006D005200120053006E006C>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 74.05500031 Tm [<003B00130058>] TJ ET EMC /P <> BDC BT 1 0 0 -1 31.5 74.05500031 Tm [<006F002F001000520037006300640046003B004500700025006A00530071007200730074003700750076>] TJ ET EMC /P <> BDC BT 1 0 0 -1 249.6499939 74.05500031 Tm [<002C>] TJ ET EMC /P <> BDC BT 1 0 0 -1 257.79998779 74.05500031 Tm [<000F>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 268.29998779 74.05500031 Tm [(1)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 273.54998779 74.05500031 Tm [<001300780079007A>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 315.54998779 74.05500031 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 320.79998779 74.05500031 Tm [(GC)] TJ ET EMC /P <> BDC BT 1 0 0 -1 331.29998779 74.05500031 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 336.54998779 74.05500031 Tm [<007B007C007D0010>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 378.54998779 74.05500031 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 383.79998779 74.05500031 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 399.54998779 74.05500031 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 404.79998779 74.05500031 Tm [<0037>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 89.65499878 Tm [<00560057005F007E>] TJ ET EMC /P <> BDC BT 1 0 0 -1 40.82500076 89.65499878 Tm [<007F>] TJ ET EMC /P <> BDC BT 1 0 0 -1 50.15000153 89.65499878 Tm [<000F>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 60.65000153 89.65499878 Tm [(2)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 65.90000153 89.65499878 Tm [<001300780079007A008000810082>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 139.3999939 89.65499878 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 142.0249939 89.65499878 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 157.7749939 89.65499878 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 160.3999939 89.65499878 Tm [<0037000A008300840085>] TJ ET EMC /P <> BDC BT 1 0 0 -1 211.7250061 89.65499878 Tm [<007F>] TJ ET EMC /P <> BDC BT 1 0 0 -1 221.05000305 89.65499878 Tm [<000F>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 231.55000305 89.65499878 Tm [(3)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 236.80000305 89.65499878 Tm [<00130050002000210086008700880089008A002200780010007A008B005600570037>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 105.25499725 Tm [<008C0086005F007E007F000F>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 63 105.25499725 Tm [(4)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 68.25 105.25499725 Tm [<0013008E008F007A>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 110.25 105.25499725 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 115.5 105.25499725 Tm [(dsRNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 141.75 105.25499725 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 147 105.25499725 Tm [<009200180089003700930083002200940095007A000A0083>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 273 105.25499725 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 278.25 105.25499725 Tm [(RNA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 294 105.25499725 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 299.25 105.25499725 Tm [<0037009600970098003B0058>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 362.25 105.25499725 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 202.80000305 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 279 89.55000305 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 296.3999939 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 312 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<006C>-4<003B>-4<0099>-4<009A>] TJ ET EMC /P <> BDC BT 1 0 0 -1 41.02600098 11.65499973 Tm [<002C>] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 50.42599869 11.65499973 Tm 0 Tr [<0050009B009C00630064001C009D00530022>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 144.92599487 11.65499973 Tm [(50)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 155.42599487 11.65499973 Tm [<005C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 165.92599487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 171.17599487 11.65499973 Tm [(1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 176.42599487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 181.67599487 11.65499973 Tm [<009E008A009F00A0>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 223.67599487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 228.92599487 11.65499973 Tm [(1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 234.17599487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 239.42599487 11.65499973 Tm [(nmol)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 263.05099487 11.65499973 Tm [<0037>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 273.55099487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 278.80099487 11.65499973 Tm [(ATP)] TJ ET EMC /P <> BDC BT 1 0 0 -1 294.55099487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 299.80099487 11.65499973 Tm [<00A600A700A8002300A9008900AA00AB00AC0037000B>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [<007C0099009A0062002E0072006C003B00AD00AE>] TJ ET EMC /P <> BDC BT 1 0 0 -1 105 27.25500107 Tm [<0058>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 110.25 27.25500107 Tm [(1X)] TJ ET EMC /P <> BDC BT 1 0 0 -1 120.75 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 126 27.25500107 Tm [(T7)] TJ ET EMC /P <> BDC BT 1 0 0 -1 136.5 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 141.75 27.25500107 Tm [(Transcription)] TJ ET EMC /P <> BDC BT 1 0 0 -1 210 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 215.25 27.25500107 Tm [(Buffer)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 246.75 27.25500107 Tm [<002C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 254.625 27.25500107 Tm [(40)] TJ ET EMC /P <> BDC BT 1 0 0 -1 265.125 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 270.375 27.25500107 Tm [(mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 280.875 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 286.125 27.25500107 Tm [(Tris)] TJ ET EMC /P <> BDC BT 1 0 0 -1 307.125 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 312.375 27.25500107 Tm [(\(pH)] TJ ET EMC /P <> BDC BT 1 0 0 -1 328.125 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 333.375 27.25500107 Tm [(7.8\),)] TJ ET EMC /P <> BDC BT 1 0 0 -1 359.625 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 364.875 27.25500107 Tm [(20)] TJ ET EMC /P <> BDC BT 1 0 0 -1 375.375 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 380.625 27.25500107 Tm [(mMNaCl,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 417.375 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 42.85499954 Tm [(18mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 21 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 26.25 42.85499954 Tm [(MgCl2,2)] TJ ET EMC /P <> BDC BT 1 0 0 -1 63 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 68.25 42.85499954 Tm [(mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 78.75 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 84 42.85499954 Tm [(Spermidine)] TJ ET EMC /P <> BDC BT 1 0 0 -1 136.5 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 141.75 42.85499954 Tm [(HCl,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 162.75 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 168 42.85499954 Tm [(10)] TJ ET EMC /P <> BDC BT 1 0 0 -1 178.5 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 183.75 42.85499954 Tm [(mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 194.25 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 199.5 42.85499954 Tm [(DTT)] TJ ET EMC /P <> BDC BT 1 0 0 -1 215.25 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 220.5 42.85499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 358.79998779 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 374.3999939 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<000B>-4<00C2>-4<00C3>-4<00C4>-4<002C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr [(10)] TJ ET EMC /P <> BDC BT 1 0 0 -1 63.20999908 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 66.23500061 11.65499973 Tm [(mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 76.73500061 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 79.76000214 11.65499973 Tm [(Tris-HCl,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 127.01000214 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 130.03500366 11.65499973 Tm [(0.1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 145.78500366 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 148.80999756 11.65499973 Tm [(M)] TJ ET EMC /P <> BDC BT 1 0 0 -1 154.05999756 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 157.08500671 11.65499973 Tm [(NaCl,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 183.33500671 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 186.36000061 11.65499973 Tm [(1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 191.61000061 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 194.63499451 11.65499973 Tm [(mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 205.13499451 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 208.16000366 11.65499973 Tm [(DTT,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 229.16000366 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 232.18499756 11.65499973 Tm [(0.1mM)] TJ ET EMC /P <> BDC BT 1 0 0 -1 258.43499756 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 261.45999146 11.65499973 Tm [(EDTA,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 287.70999146 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 290.73498535 11.65499973 Tm [(50%)] TJ ET EMC /P <> BDC BT 1 0 0 -1 306.48498535 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 309.51000977 11.65499973 Tm [(Glycerol,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 356.76000977 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 359.78500366 11.65499973 Tm [(0.01%)] TJ ET EMC /P <> BDC BT 1 0 0 -1 386.03500366 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 389.05999756 11.65499973 Tm [(\(w/v\))] TJ ET EMC /P <> BDC BT 1 0 0 -1 415.30999756 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [(Triton)] TJ ET EMC /P <> BDC BT 1 0 0 -1 31.5 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 36.75 27.25500107 Tm [(X-100,)] TJ ET EMC /P <> BDC BT 1 0 0 -1 68.25 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 73.5 27.25500107 Tm [(pH)] TJ ET EMC /P <> BDC BT 1 0 0 -1 84 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 89.25 27.25500107 Tm [(7.5)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 105 27.25500107 Tm [<0058>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 115.5 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 120.75 27.25500107 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 405.6000061 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 421.20001221 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<00C2>-4<00C3>-4<002C>] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 31.62599945 11.65499973 Tm 0 Tr [<00CA0025>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 52.62599945 11.65499973 Tm [(-20)] TJ ET EMC /P <> BDC BT 1 0 0 -1 68.37599945 11.65499973 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 78.87599945 11.65499973 Tm [(C)] TJ ET EMC /P <> BDC BT 1 0 0 -1 84.12599945 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 89.37599945 11.65499973 Tm [<004F006700C3>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 120.87599945 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 126.12599945 11.65499973 Tm [(2)] TJ ET EMC /P <> BDC BT 1 0 0 -1 131.37600708 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 136.62600708 11.65499973 Tm [<00CC002200CD0096006300CE00CF00D00058>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 231.12600708 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 236.37600708 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 436.79998779 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 452.3999939 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<0006>-4<00D1>-4<006C>] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 31.62599945 11.65499973 Tm 0 Tr [<002C>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 42.12599945 11.65499973 Tm [(75)] TJ ET EMC /P <> BDC BT 1 0 0 -1 52.62599945 11.65499973 Tm [(?] TJ ET EMC /P <> BDC BT 1 0 0 -1 63.12599945 11.65499973 Tm [(C)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 68.37599945 11.65499973 Tm [<0022>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 78.87599945 11.65499973 Tm [(10min)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 105.12599945 11.65499973 Tm [<0058>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 115.62599945 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 468 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 483.6000061 cm /P <> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<00D2>-4<00D3>-4<0073>-4<00D4>] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 42.16799927 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 47.41799927 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 499.20001221 cm /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(1.)] TJ ET EMC /P <> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 10.5 11.65499973 Tm [<00D500D600630064001C009D>] TJ ET EMC /P <> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 73.5 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 90 333.29400635 cm /P <> BDC q 1 0 0 1 0 4.05000305 cm q 279 0 0 -85.5 0 85.5 cm /X3 Do Q Q EMC Q 1 0 0 -1 0 841.90002441 cm endstream endobj 6 0 obj <>stream ÿ??ºÝºÝÈÈÔÚÏßÊÓÆµÃâ·Ñ <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| 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