%PDF-1.7 %ÈÈÈÈÈÈ?1 0 obj <> endobj 2 0 obj <>/Metadata 141 0 R>> endobj 3 0 obj <> endobj 4 0 obj <>/ExtGState<>/XObject<>>>/Group<>/StructParents 0/Tabs/S>> endobj 5 0 obj <>stream 1 0 0 -1 0 841.90002441 cm q 1 g 0 0 m 600 0 l 600 450 l 0 450 l h f* Q q 1 0 0 1 0 450 cm q 1 g 0 0 m 600 0 l 600 450 l 0 450 l h f* Q Q q 1 0 0 1 65.65000153 93.5490036 cm q 474.8500061 0 0 -20.79999924 0 20.79999924 cm /X1 Do Q Q /Artifact <> BDC q 1 0 0 1 90 42.54999924 cm -90 0 m 505.29998779 0 l 505.29998779 95.16999817 l -90 95.16999817 l h W n BT /FAAAAI 9 Tf 1 0 0 -1 0 9.40200043 Tm 0 g [( )] TJ /GS1 gs 0 g [-8750( )] TJ ET q 1 0 0 1 0 11.34899998 cm BT 1 0 0 -1 0 41.42499924 Tm /GS2 gs 0 g [( )] TJ 1 0 0 -1 413.36099243 41.42499924 Tm /GS1 gs 0 g [( )] TJ ET Q q 1 0 0 1 0 52.77399826 cm BT 1 0 0 -1 0 8.40200043 Tm /GS2 gs 0 g [( )] TJ /GS1 gs 0 g [-8250( )] TJ ET Q q 1 0 0 1 0 63.1230011 cm BT 1 0 0 -1 0 8.40200043 Tm [( )] TJ ET Q q 1 0 0 1 0 73.47200012 cm BT 1 0 0 -1 0 8.40200043 Tm /GS2 gs 0 g [( )] TJ /FAAABD 9 Tf [-500( )] TJ /GS1 gs 0 g [-250( )] TJ /FAAAAI 9 Tf 1 0 0 -1 381.29998779 8.40200043 Tm [( )] TJ ET Q q 1 0 0 1 0 83.82099915 cm Q Q EMC /Artifact <> BDC q 1 0 0 1 90 781.95098877 cm -90 0 m 505.29998779 0 l 505.29998779 10.34899998 l -90 10.34899998 l h W n Q EMC q 1 0 0 1 90 53.89899826 cm q 1 0 0 1 225 -0.00000076 cm q 188.3614502 0 0 -41.42535019 0 41.42535019 cm /X2 Do Q Q Q q 1 0 0 1 90 137.72000122 cm /P <> BDC BT /FAAABD 16 Tf 1 0 0 -1 124.54299927 21.47500038 Tm 0 g [(RIPA)] TJ ET EMC /P <> BDC BT /FAAABH 16 Tf 1 0 0 -1 167.6519928 21.47500038 Tm 2 Tr 0.45714286 w 0 G [<0003>-4<0004>-4<0005>] TJ ET EMC /P <> BDC BT /FAAABD 16 Tf 1 0 0 -1 215.84399414 21.47500038 Tm 0 Tr [(\()] TJ ET EMC /P <> BDC BT /FAAABH 16 Tf 1 0 0 -1 221.17199707 21.47500038 Tm 2 Tr [<0006>] TJ ET EMC /P <> BDC BT /FAAABD 16 Tf 1 0 0 -1 237.23599243 21.47500038 Tm 0 Tr [(\))] TJ ET EMC /P <> BDC BT /FAAABH 16 Tf 1 0 0 -1 242.56399536 21.47500038 Tm 2 Tr [<0007>-4<0008>-4<0009>] TJ ET EMC /P <> BDC BT /FAAABD 16 Tf 1 0 0 -1 290.75601196 21.47500038 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC q 1 0 0 1 0 31.20000076 cm /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 207.6499939 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 46.79999924 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 102.23000336 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<000A>-4<000B>-4<000C>-4<000D>-4<000E>-4<000F>-3<0010>-4<0011>-4<0012>-4<0013>-4<0014>-4<0015>-4<0016>-4<0017>-4<0014>-4<0018>-3<0019>-4<001A>-4<001B>-4<001C>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 313.07000732 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 62.40000153 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 78 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<000B>-4<000C>-4<001D>-4<001E>-4<001F>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 93.59999847 cm /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [<00200021002200230024002500260027002800290006002A002B002C002D002E>] TJ ET EMC /P <> BDC BT 1 0 0 -1 168 11.65499973 Tm [<0015>] TJ ET EMC /P <> BDC BT 1 0 0 -1 175.80700684 11.65499973 Tm [<002F>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 186.30700684 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 191.55700684 11.65499973 Tm [(Triton)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 217.80200195 11.65499973 Tm [<0030>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 225.60899353 11.65499973 Tm [(SDS)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 244.8710022 11.65499973 Tm [<0030>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 252.67799377 11.65499973 Tm [(NP-40)] TJ ET EMC /P <> BDC BT 1 0 0 -1 280.09698486 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 285.34698486 11.65499973 Tm [<0031>] TJ ET EMC /P <> BDC BT 1 0 0 -1 295.84698486 11.65499973 Tm [<0032>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 303.6539917 11.65499973 Tm [(RIPA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 327.57598877 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 332.82598877 11.65499973 Tm [<000300040005>] TJ ET EMC /P <> BDC BT 1 0 0 -1 361.63299561 11.65499973 Tm [<003300060034>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 393.13299561 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 398.38299561 11.65499973 Tm [(\(RIPA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 425.80200195 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [(Lysis)] TJ ET EMC /P <> BDC BT 1 0 0 -1 22.75300026 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 25.43499947 27.25500107 Tm [(Buffer\))] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 56.39199829 27.25500107 Tm [<0035>-5<0036>-6<0014>-5<0037>-6<0021>-5<0038>-6<0039>-5<003A>-5<0028>-6<0029>-5<003B>-6<003C>-5<003D>-6<003E>-5<0003>-5<0004>-6<003F>-5<0040>-6<0017>-5<002C>-6<002D>-5<002E>-5<003A>-6<0003>-5<0004>-6<0005>-5<0015>-6<0041>-5<0003>-5<0004>-6<0042>-5<0043>-6<0044>-5<0018>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 415.32998657 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 42.85499954 Tm [(NP-40)] TJ ET EMC /P <> BDC BT 1 0 0 -1 27.41900063 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 32.89400101 42.85499954 Tm [<0003>-21<0004>-22<0005>-21<0030>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 75.79399872 42.85499954 Tm [(RIPA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 99.71600342 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 105.19100189 42.85499954 Tm [<0003>-21<0004>-22<0005>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 137.36599731 42.85499954 Tm [(\()] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 141.08799744 42.85499954 Tm [<0045>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 151.81300354 42.85499954 Tm [(\))] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 155.53500366 42.85499954 Tm [<0032>-21<0046>-22<0040>-21<0017>-22<003A>-21<002D>-22<002E>-21<0047>-21<0025>-22<0014>-21<0018>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 273.51000977 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 278.98498535 42.85499954 Tm [(Western)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 314.19100952 42.85499954 Tm [<0015>-21<0048>-22<0049>-21<004A>-22<004B>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 367.81600952 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 373.29098511 42.85499954 Tm [(\(Immunol)] TJ ET EMC /P <> BDC BT 1 0 0 -1 415.28601074 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 58.45499802 Tm [(Precipit)1(ation)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 53.65399933 58.45499802 Tm [<0015>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 64.15399933 58.45499802 Tm [(IP\))] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 76.98699951 58.45499802 Tm [<00310032>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 97.98699951 58.45499802 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 156 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 171.6000061 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Medium)] TJ ET EMC /P <> BDC BT 1 0 0 -1 35.58100128 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 38.20600128 11.65499973 Tm [(RIPA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 62.12799835 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 64.75299835 11.65499973 Tm [(Lysis)] TJ ET EMC /P <> BDC BT 1 0 0 -1 87.5059967 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 90.1309967 11.65499973 Tm [(Buffer)] TJ ET EMC /P <> BDC BT 1 0 0 -1 117.53500366 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 122.78500366 11.65499973 Tm [<004C004D004E>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 154.28500366 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 159.53500366 11.65499973 Tm [(Tris)] TJ ET EMC /P <> BDC BT 1 0 0 -1 176.44900513 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 181.69900513 11.65499973 Tm [<0030>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 192.19900513 11.65499973 Tm [(NP-40)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 219.61799622 11.65499973 Tm [<0030>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 230.11799622 11.65499973 Tm [(sodium)] TJ ET EMC /P <> BDC BT 1 0 0 -1 261.03900146 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 263.66400146 11.65499973 Tm [(deoxychola)1(te)] TJ ET EMC /P <> BDC BT 1 0 0 -1 319.64001465 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 324.89001465 11.65499973 Tm [<0031003B00220015003F004F005000200021>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [<002D002E0051005200530054002200230015002500260050005500520053002D002E003A005600040015003F0057005800590050003A002D002E005A005B005C005D00140032>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 367.5 27.25500107 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 202.80000305 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 218.3999939 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<000B>-4<000C>-4<003B>-4<0022>-4<001F>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 234 cm /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 -10 249.6000061 cm q 0.25 0.5 m 127.90000153 0.5 l 127.90000153 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAABH 9 Tf 1 0 0 -1 40.60300064 11.20499992 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<000B>-4<000C>-4<005E>-4<005F>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 76.74700165 11.20499992 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 128.1499939 0 cm 0.25 0.5 m 53.29999924 0.5 l 53.29999924 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAABH 9 Tf 1 0 0 -1 12.33899975 11.20499992 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0060>-4<0061>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 30.4109993 11.20499992 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 181.69999695 0 cm 0.25 0.5 m 58.54999924 0.5 l 58.54999924 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAABH 9 Tf 1 0 0 -1 5.92799997 11.20499992 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0062>-4<0063>-4<0064>-4<0065>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 42.0719986 11.20499992 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 240.5 0 cm 0.25 0.5 m 46.5 0.5 l 46.5 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAABH 9 Tf 1 0 0 -1 4.421 11.20499992 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0007>-4<0008>-4<0009>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 31.52899933 11.20499992 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 287.25 0 cm 0.25 0.5 m 49.5 0.5 l 49.5 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAABH 9 Tf 1 0 0 -1 5.921 11.20499992 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0050>-4<0055>-4<0066>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 33.02899933 11.20499992 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 16.29999924 cm q 0.25 0.5 m 127.90000153 0.5 l 127.90000153 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 26.29999924 11.20499992 Tm /GS2 gs 0 g [(RIPA)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 49.05500031 11.20499992 Tm [<000300040005>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 76.05500031 11.20499992 Tm [(\()] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 79.05200195 11.20499992 Tm [<0006>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 88.05200195 11.20499992 Tm [(\))] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 91.0490036 11.20499992 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 128.1499939 0 cm 0.25 0.5 m 53.29999924 0.5 l 53.29999924 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 10.9989996 11.12700081 Tm /GS2 gs 0 g [(1.5ml)] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 31.75 11.12700081 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 181.69999695 0 cm 0.25 0.5 m 58.54999924 0.5 l 58.54999924 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 13.5010004 11.20499992 Tm /GS2 gs 0 g [(-20)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 25.49799919 11.20499992 Tm [<0067>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 34.4980011 11.20499992 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 240.5 0 cm 0.25 0.5 m 46.5 0.5 l 46.5 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 10.10000038 11.20499992 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 16.85000038 11.20499992 Tm [<0068>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 25.85000038 11.20499992 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 287.25 0 cm 0.25 0.5 m 49.5 0.5 l 49.5 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 11.60000038 11.20499992 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 18.35000038 11.20499992 Tm [<0069>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 27.35000038 11.20499992 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 32.59999847 cm q 0.25 0.5 m 127.90000153 0.5 l 127.90000153 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 8.30200005 11.12700081 Tm /GS2 gs 0 g [(Medium)] TJ ET EMC /P <> BDC BT 1 0 0 -1 38.79999924 11.12700081 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 41.04999924 11.12700081 Tm [(RIPA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 61.55500031 11.12700081 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 63.80500031 11.12700081 Tm [(Lysis)] TJ ET EMC /P <> BDC BT 1 0 0 -1 83.30799866 11.12700081 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 85.55799866 11.12700081 Tm [(Buffer)] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 109.04699707 11.12700081 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 128.1499939 0 cm 0.25 0.5 m 53.29999924 0.5 l 53.29999924 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 9.8739996 11.12700081 Tm /GS2 gs 0 g [(100ml)] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 32.875 11.12700081 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 181.69999695 0 cm 0.25 0.5 m 58.54999924 0.5 l 58.54999924 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 13.5010004 11.20499992 Tm /GS2 gs 0 g [(-20)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 25.49799919 11.20499992 Tm [<0067>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 34.4980011 11.20499992 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 240.5 0 cm 0.25 0.5 m 46.5 0.5 l 46.5 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 10.10000038 11.20499992 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 16.85000038 11.20499992 Tm [<0068>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 25.85000038 11.20499992 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 287.25 0 cm 0.25 0.5 m 49.5 0.5 l 49.5 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 11.60000038 11.20499992 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 18.35000038 11.20499992 Tm [<0069>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 27.35000038 11.20499992 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 48.90000153 cm q 0.25 0.5 m 127.90000153 0.5 l 127.90000153 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 29.91699982 11.12700081 Tm /GS2 gs 0 g [(PMSF)-1(\(100m)1(M)-1(\))] TJ ET EMC /P <> BDC BT 1 0 0 -1 87.43299866 11.12700081 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 128.1499939 0 cm 0.25 0.5 m 53.29999924 0.5 l 53.29999924 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 10.9989996 11.12700081 Tm /GS2 gs 0 g [(1.5ml)] TJ ET EMC /P <> BDC BT 1 0 0 -1 31.75 11.12700081 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 181.69999695 0 cm 0.25 0.5 m 58.54999924 0.5 l 58.54999924 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 13.5010004 11.20499992 Tm /GS2 gs 0 g [(-20)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 25.49799919 11.20499992 Tm [<0067>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 34.4980011 11.20499992 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 240.5 0 cm 0.25 0.5 m 46.5 0.5 l 46.5 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 10.10000038 11.20499992 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 16.85000038 11.20499992 Tm [<0068>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 25.85000038 11.20499992 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 287.25 0 cm 0.25 0.5 m 49.5 0.5 l 49.5 16.29999924 l 0.25 16.29999924 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 11.60000038 11.20499992 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 18.35000038 11.20499992 Tm [<0069>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 27.35000038 11.20499992 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0.5 w 0 0.5 m 0 65.19999695 l S Q q 0.5 w 128.1499939 0.5 m 128.1499939 16.29999924 l S Q q 0.5 w 128.1499939 16.79999924 m 128.1499939 32.59999847 l S Q q 0.5 w 128.1499939 33.09999847 m 128.1499939 48.89999771 l S Q q 0.5 w 128.1499939 49.39999771 m 128.1499939 65.19999695 l S Q q 0.5 w 181.69999695 0.5 m 181.69999695 16.29999924 l S Q q 0.5 w 181.69999695 16.79999924 m 181.69999695 32.59999847 l S Q q 0.5 w 181.69999695 33.09999847 m 181.69999695 48.89999771 l S Q q 0.5 w 181.69999695 49.39999771 m 181.69999695 65.19999695 l S Q q 0.5 w 240.5 0.5 m 240.5 16.29999924 l S Q q 0.5 w 240.5 16.79999924 m 240.5 32.59999847 l S Q q 0.5 w 240.5 33.09999847 m 240.5 48.89999771 l S Q q 0.5 w 240.5 49.39999771 m 240.5 65.19999695 l S Q q 0.5 w 287.25 0.5 m 287.25 16.29999924 l S Q q 0.5 w 287.25 16.79999924 m 287.25 32.59999847 l S Q q 0.5 w 287.25 33.09999847 m 287.25 48.89999771 l S Q q 0.5 w 287.25 49.39999771 m 287.25 65.19999695 l S Q q 0.5 w 337 0.5 m 337 65.19999695 l S Q q 0.5 w -0.25 0.25 m 337.25 0.25 l S Q q 0.5 w 0.25 16.54999924 m 336.75 16.54999924 l S Q q 0.5 w 0.25 32.84999847 m 336.75 32.84999847 l S Q q 0.5 w 0.25 49.14999771 m 336.75 49.14999771 l S Q q 0.5 w -0.25 65.44999695 m 337.25 65.44999695 l S Q Q q 1 0 0 1 0 315.29998779 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 330.8999939 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<006A>-4<005D>-4<006B>-4<006C>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 42.16799927 11.65499973 Tm 0 Tr [(\()] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 45.66500092 11.65499973 Tm 2 Tr [<000D>-4<000E>-4<006D>-4<006E>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 87.83300018 11.65499973 Tm 0 Tr [(\))] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 91.33000183 11.65499973 Tm 2 Tr [<001F>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 101.87200165 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 346.5 cm /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(\()] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 3.49699998 11.65499973 Tm 2 Tr 0.30000001 w [<0037>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 14.03899956 11.65499973 Tm 0 Tr [(\))] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 17.5359993 11.65499973 Tm 2 Tr [<006F>-4<0070>-4<0071>-4<0072>-4<0028>-4<0029>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 80.78800201 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 362.1000061 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 6.12200022 11.65499973 Tm [<0030>-83<002B>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 28.86599922 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 34.98799896 11.65499973 Tm [(Medium)] TJ ET EMC /P <> BDC BT 1 0 0 -1 70.56900024 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 74.06600189 11.65499973 Tm [(RIPA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 97.98799896 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 101.48500061 11.65499973 Tm [(Lysis)] TJ ET EMC /P <> BDC BT 1 0 0 -1 124.23799896 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 127.73500061 11.65499973 Tm [(Buffer)] TJ ET EMC /P <> BDC BT 1 0 0 -1 155.13900757 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 161.26100159 11.65499973 Tm [<0073>-83<0074>-83<0075>-83<0076>-83<0004>-83<0077>-83<0078>-83<0015>-83<0079>-83<007A>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 274.98098755 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 281.10299683 11.65499973 Tm [(PMSF)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 308.82998657 11.65499973 Tm [<0015>-83<0013>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 331.57400513 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 337.6960144 11.65499973 Tm [(PMSF)] TJ ET EMC /P <> BDC BT 1 0 0 -1 364.55099487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 370.67300415 11.65499973 Tm [<007B>-83<007C>-83<0043>-83<007D>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 416.16101074 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [(1mM)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 22.75300026 27.25500107 Tm [<0032>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 33.25299835 27.25500107 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 393.29998779 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(2)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<0030007E007F006F007000280029003A00710072000500150014>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 141.75 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 147 11.65499973 Tm [(PBS)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 165.68299866 11.65499973 Tm [<0030>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 176.18299866 11.65499973 Tm [(NS)] TJ ET EMC /P <> BDC BT 1 0 0 -1 189.60499573 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 194.85499573 11.65499973 Tm [<008000810082008300710072000500830084>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 289.35501099 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 294.60501099 11.65499973 Tm [(1)] TJ ET EMC /P <> BDC BT 1 0 0 -1 299.85501099 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 305.10501099 11.65499973 Tm [<008500150086003E0087008800150089008A00830015>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 420.60501099 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 408.8999939 cm /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [<008B002B004A004B0032>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 52.5 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 424.5 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(3)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<0030>] TJ ET EMC /P <> BDC BT 1 0 0 -1 13.82400036 11.65499973 Tm [<008C008D>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 34.82400131 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 40.07400131 11.65499973 Tm [(6)] TJ ET EMC /P <> BDC BT 1 0 0 -1 45.32400131 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 50.57400131 11.65499973 Tm [<008E008F0090008E0079007A>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 113.5739975 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 118.8239975 11.65499973 Tm [(150)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 134.57400513 11.65499973 Tm [<0091>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 145.07400513 11.65499973 Tm [(250)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 160.82400513 11.65499973 Tm [<0092>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 171.32400513 11.65499973 Tm [(l)] TJ ET EMC /P <> BDC BT 1 0 0 -1 174.24099731 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 179.49099731 11.65499973 Tm [<004F0050>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 200.49099731 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 205.74099731 11.65499973 Tm [(PMSF)] TJ ET EMC /P <> BDC BT 1 0 0 -1 232.59599304 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 237.84599304 11.65499973 Tm [<003A000300040005003A00930094>] TJ ET EMC /P <> BDC BT 1 0 0 -1 311.3460083 11.65499973 Tm [<0015>] TJ ET EMC /P <> BDC BT 1 0 0 -1 319.92001343 11.65499973 Tm [<0079007A>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 340.92001343 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 346.17001343 11.65499973 Tm [(Medium)] TJ ET EMC /P <> BDC BT 1 0 0 -1 381.75100708 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 384.37600708 11.65499973 Tm [(RIPA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 408.29800415 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 410.92300415 11.65499973 Tm [(Lysis)] TJ ET EMC /P <> BDC BT 1 0 0 -1 433.67599487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [(Buffer)] TJ ET EMC /P <> BDC BT 1 0 0 -1 27.40399933 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 32.76599884 27.25500107 Tm [<0032>-11<0095>-10<0005>-11<0096>-11<0097>-10<0097>-11<0098>-11<0099>-10<0015>-11<0013>-11<0003>-10<0004>-11<0005>-11<009A>-10<0028>-11<0029>-11<009B>-10<0023>-11<009C>-11<009D>-10<0032>-11<0073>-11<0018>-10<009E>-11<008A>-11<0080>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 308.67800903 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 314.04000854 27.25500107 Tm [(4)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 319.40200806 27.25500107 Tm [<0067>-11<0003>-10<0004>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 351.23800659 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 356.6000061 27.25500107 Tm [(15)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 367.21200562 27.25500107 Tm [<0091>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 377.82400513 27.25500107 Tm [(30min)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 404.76998901 27.25500107 Tm [<0015>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 42.85499954 Tm [<009F00A0000300040005005D0014001800280029>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 105 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 110.25 42.85499954 Tm [(1)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 115.5 42.85499954 Tm [<0091>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 126 42.85499954 Tm [(3)] TJ ET EMC /P <> BDC BT 1 0 0 -1 131.25 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 136.5 42.85499954 Tm [<00A100A200150028002900A300A400A5000300040032009F00A0>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 273 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 278.25 42.85499954 Tm [(6)] TJ ET EMC /P <> BDC BT 1 0 0 -1 283.5 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 288.75 42.85499954 Tm [<008E008F0090008E002800290079007A>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 375.375 42.85499954 Tm [(100)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 391.125 42.85499954 Tm [<0092>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 401.625 42.85499954 Tm [(l)] TJ ET EMC /P <> BDC BT 1 0 0 -1 404.54199219 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 409.79199219 42.85499954 Tm [<0004>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 58.45499802 Tm [<000500A6003800A700A80015002F00A90028002900AA004300AB00AC0025002600AD00AE00790044000300040005003A001400AF00B0>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 283.5 58.45499802 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 288.75 58.45499802 Tm [(200)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 304.5 58.45499802 Tm [<0091>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 315 58.45499802 Tm [(250)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 330.75 58.45499802 Tm [<0092>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 341.25 58.45499802 Tm [(l)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 344.16699219 58.45499802 Tm [<0032>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 354.66699219 58.45499802 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 486.8999939 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(4)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<0030>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 15.75 11.65499973 Tm [(10000)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 42 11.65499973 Tm [<0091>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 52.5 11.65499973 Tm [(12000g)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 84 11.65499973 Tm [<0015>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 94.5 11.65499973 Tm [(4)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 99.75 11.65499973 Tm [<006700870088>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 131.25 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 136.5 11.65499973 Tm [(5)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 141.75 11.65499973 Tm [<0091>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 152.25 11.65499973 Tm [(10min\()] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 182.58099365 11.65499973 Tm [<002F0081008600750087008800B100150074007500B20087008800B30025>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 340.08099365 11.65499973 Tm [(\))] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 343.57800293 11.65499973 Tm [<0015002B008A00830032>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 396.07800293 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 502.5 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(5)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<003000B400B500B600B7003A>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 68.25 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 73.5 11.65499973 Tm [(SDS-P)-1(AGE)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 123.67700195 11.65499973 Tm [<0030>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 134.17700195 11.65499973 Tm [(Western)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 169.15800476 11.65499973 Tm [<003000480049004A004B009A0048004900B8004A004B0031006A005D0032>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 326.6579895 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 518.09997559 cm /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(\()] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 3.49699998 11.65499973 Tm 2 Tr 0.30000001 w [<00B9>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 14.03899956 11.65499973 Tm 0 Tr [(\))] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 17.5359993 11.65499973 Tm 2 Tr [<00BA>-4<00BB>-4<0071>-4<0072>-4<0028>-4<0029>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 80.78800201 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 533.70001221 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 6.12200022 11.65499973 Tm [<0030>-83<002B>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 28.86599922 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 34.98799896 11.65499973 Tm [(Medium)] TJ ET EMC /P <> BDC BT 1 0 0 -1 70.56900024 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 74.06600189 11.65499973 Tm [(RIPA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 97.98799896 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 101.48500061 11.65499973 Tm [(Lysis)] TJ ET EMC /P <> BDC BT 1 0 0 -1 124.23799896 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 127.73500061 11.65499973 Tm [(Buffer)] TJ ET EMC /P <> BDC BT 1 0 0 -1 155.13900757 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 161.26100159 11.65499973 Tm [<0073>-83<0074>-83<0075>-83<0076>-83<0004>-83<0077>-83<0078>-83<0015>-83<0079>-83<007A>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 274.98098755 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 281.10299683 11.65499973 Tm [(PMSF)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 308.82998657 11.65499973 Tm [<0015>-83<0013>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 331.57400513 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 337.6960144 11.65499973 Tm [(PMSF)] TJ ET EMC /P <> BDC BT 1 0 0 -1 364.55099487 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 370.67300415 11.65499973 Tm [<007B>-83<007C>-83<0043>-83<007D>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 416.16101074 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [(1mM)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 22.75300026 27.25500107 Tm [<0032>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 33.25299835 27.25500107 Tm [(2)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 38.50299835 27.25500107 Tm [<00300086003E0087008800BA00BB0028002900150089008A0083001500BC00BD004A004B0032>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 238.00300598 27.25500107 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 564.90002441 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(3)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<0030001400BE00BF009700C00028002900150013004100C100C20032008C008D>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 173.25 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 178.5 11.65499973 Tm [(6)] TJ ET EMC /P <> BDC BT 1 0 0 -1 183.75 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 189 11.65499973 Tm [<008E008F0090008E002800290079007A>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 273 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 278.25 11.65499973 Tm [(150)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 294 11.65499973 Tm [<0091>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 304.5 11.65499973 Tm [(250)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 320.25 11.65499973 Tm [<0092>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 330.75 11.65499973 Tm [(l)] TJ ET EMC /P <> BDC BT 1 0 0 -1 333.66699219 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 338.91699219 11.65499973 Tm [<004F0050>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 359.91699219 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 365.16699219 11.65499973 Tm [(PMSF)] TJ ET EMC /P <> BDC BT 1 0 0 -1 392.02200317 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 397.27200317 11.65499973 Tm [<003A0003>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [<0004>-2<0005>-3<003A>-2<0093>-3<0094>-2<0015>-3<0079>-2<007A>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 84.20800018 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 89.48400116 27.25500107 Tm [(Medium)] TJ ET EMC /P <> BDC BT 1 0 0 -1 125.06500244 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 127.71600342 27.25500107 Tm [(RIPA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 151.63800049 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 154.28900146 27.25500107 Tm [(Lysis)] TJ ET EMC /P <> BDC BT 1 0 0 -1 177.04200745 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 179.69299316 27.25500107 Tm [(Buffer)] TJ ET EMC /P <> BDC BT 1 0 0 -1 207.09700012 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 212.3730011 27.25500107 Tm [<0032>-2<009F>-3<00A0>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 243.95100403 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 249.227005 27.25500107 Tm [(6)] TJ ET EMC /P <> BDC BT 1 0 0 -1 254.477005 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 259.75299072 27.25500107 Tm [<008E>-2<008F>-3<0090>-2<008E>-3<0028>-2<0029>-3<0079>-2<007A>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 343.96099854 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 349.23699951 27.25500107 Tm [(150)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 365.01300049 27.25500107 Tm [<0092>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 375.53900146 27.25500107 Tm [(l)] TJ ET EMC /P <> BDC BT 1 0 0 -1 378.45599365 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 383.73199463 27.25500107 Tm [<0003>-2<0004>-3<0005>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 42.85499954 Tm [<00A6003800A700A8001500C3002F00A90028002900AA004300C400A000AC0025002600AD00AE00790044000300040005003A001400AF00B0>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 294 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 299.25 42.85499954 Tm [(200)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 315 42.85499954 Tm [<0091>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 325.5 42.85499954 Tm [(250)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 341.25 42.85499954 Tm [<0092>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 351.75 42.85499954 Tm [(l)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 354.66699219 42.85499954 Tm [<003200C5001400BE>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 396.66699219 42.85499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 611.70001221 cm /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [<00BF009700C00026009B002300030004002800290015009B00230003000400B600C600C70050000800C8003A00280029004A004B0032>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 283.5 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q 1 0 0 -1 0 841.90002441 cm endstream endobj 6 0 obj <>stream ÿ??ºÝºÝÈÈÔÚÏßÊÓÆµÃâ·Ñ <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Îı¾Á´> <Îı¾Á´> <Îı¾Á´> <Îı¾Á´> <Îı¾Á´> <Îı¾Á´>