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8.40200043 Tm /GS2 gs 0 g [(  )] TJ /FAAABD 9 Tf [-500( )] TJ /FAAAAI 9 Tf /GS1 gs 0 g [-250( )] TJ ET Q q 1 0 0 1 0 83.82099915 cm Q Q EMC /Artifact <</Attached [/Bottom ] /BBox [90 49.60003662 505.29998779 59.94903564] /Type /Pagination >> BDC q 1 0 0 1 90 781.95098877 cm -90 0 m 505.29998779 0 l 505.29998779 10.34899998 l -90 10.34899998 l h W n Q EMC q 1 0 0 1 90 53.89899826 cm q 1 0 0 1 225 -0.00000076 cm q 188.3614502 0 0 -41.42535019 0 41.42535019 cm /X2 Do Q Q Q q 1 0 0 1 90 137.72000122 cm /P <</MCID 0 >> BDC BT /FAAABD 16 Tf 1 0 0 -1 71.90299988 21.47500038 Tm 0 g [(Tris-HCl)] TJ ET EMC /P <</MCID 1 >> BDC BT 1 0 0 -1 134.12199402 21.47500038 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 2 >> BDC BT /FAAABH 16 Tf 1 0 0 -1 142.12199402 21.47500038 Tm 2 Tr 0.45714286 w 0 G [<0003>-4<0004>-4<0005>] TJ ET EMC /P <</MCID 3 >> BDC BT /FAAABD 16 Tf 1 0 0 -1 190.31399536 21.47500038 Tm 0 Tr [(\(1mol/L,pH6.8\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 4 >> BDC BT /FAAABH 16 Tf 1 0 0 -1 295.20498657 21.47500038 Tm 2 Tr [<0006>-4<0007>-4<0008>] TJ ET EMC /P <</MCID 5 >> BDC BT /FAAABD 16 Tf 1 0 0 -1 343.39700317 21.47500038 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC q 1 0 0 1 0 31.20000076 cm /P <</MCID 6 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 207.6499939 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 46.79999924 cm /P <</Lang (zh-CN) /MCID 7 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 102.23000336 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<0009>-4<000A>-4<000B>-4<000C>-4<000D>-4<000E>-3<000F>-4<0010>-4<0011>-4<0012>-4<0013>-4<0014>-4<0015>-4<0016>-4<0013>-4<0017>-3<0018>-4<0019>-4<001A>-4<001B>] TJ ET EMC /P <</MCID 8 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 313.07000732 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 62.40000153 cm /P <</MCID 9 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 78 cm /P <</Lang (zh-CN) /MCID 10 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<000A>-4<000B>-4<001C>-4<001D>-4<001E>] TJ ET EMC /P <</MCID 11 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 93.59999847 cm /P <</MCID 12 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris)] TJ ET EMC /P <</MCID 13 >> BDC BT 1 0 0 -1 16.91399956 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 14 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 22.16399956 11.65499973 Tm [<001F002000210014002200230024001F>] TJ ET EMC /P <</MCID 15 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 106.16400146 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 16 >> BDC BT 1 0 0 -1 111.41400146 11.65499973 Tm [(C4H11NO3)] TJ ET EMC /P <</Lang (zh-CN) /MCID 17 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 162.16600037 11.65499973 Tm [<001400250026002200230027001F>] TJ ET EMC /P <</MCID 18 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 235.66600037 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 19 >> BDC BT 1 0 0 -1 240.91600037 11.65499973 Tm [(121.14)] TJ ET EMC /P <</Lang (zh-CN) /MCID 20 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 269.79098511 11.65499973 Tm [<00140028>] TJ ET EMC /P <</MCID 21 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 290.79098511 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 22 >> BDC BT 1 0 0 -1 296.04098511 11.65499973 Tm [(25)] TJ ET EMC /P <</Lang (zh-CN) /MCID 23 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 306.54098511 11.65499973 Tm [<0029002A002B002C>] TJ ET EMC /P <</MCID 24 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 348.54098511 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 25 >> BDC BT 1 0 0 -1 353.79098511 11.65499973 Tm [(pKa)] TJ ET EMC /P <</MCID 26 >> BDC BT 1 0 0 -1 371.28399658 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 27 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 376.53399658 11.65499973 Tm [<001F>] TJ ET EMC /P <</MCID 28 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 387.03399658 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 29 >> BDC BT 1 0 0 -1 392.28399658 11.65499973 Tm [(8.1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 30 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 405.40899658 11.65499973 Tm [<0014>] TJ ET EMC /P <</MCID 31 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 415.90899658 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 32 >> BDC BT 1 0 0 -1 0 27.25500107 Tm [(Tris)] TJ ET EMC /P <</MCID 33 >> BDC BT 1 0 0 -1 16.91399956 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 34 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 22.2859993 27.25500107 Tm [<0003>-12<0004>-11<0005>-12<002C>-11<002D>-12<002E>-12<0003>-11<0004>-12<002F>-12<0030>-11<0028>] TJ ET EMC /P <</MCID 35 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 139.12800598 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 36 >> BDC BT 1 0 0 -1 144.5 27.25500107 Tm [(pH7.0)] TJ ET EMC /P <</Lang (zh-CN) /MCID 37 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 170.58000183 27.25500107 Tm [<0031>] TJ ET EMC /P <</MCID 38 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 181.20199585 27.25500107 Tm [(9.2)] TJ ET EMC /P <</MCID 39 >> BDC BT 1 0 0 -1 194.32699585 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 40 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 199.69900513 27.25500107 Tm [<0032>-12<0033>-11<0014>] TJ ET EMC /P <</MCID 41 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 231.56500244 27.25500107 Tm [(Tris)] TJ ET EMC /P <</MCID 42 >> BDC BT 1 0 0 -1 248.47900391 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 43 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 253.85099792 27.25500107 Tm [<0021>-12<0034>-11<0035>-12<0005>-11<002C>] TJ ET EMC /P <</MCID 44 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 306.96099854 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 45 >> BDC BT 1 0 0 -1 312.33300781 27.25500107 Tm [(pH)] TJ ET EMC /P <</MCID 46 >> BDC BT 1 0 0 -1 325.16598511 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 47 >> BDC BT 1 0 0 -1 327.91299438 27.25500107 Tm [(10.5)] TJ ET EMC /P <</MCID 48 >> BDC BT 1 0 0 -1 346.28799438 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 49 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 351.66000366 27.25500107 Tm [<0036>-12<0037>-11<0014>-12<0038>-11<0039>-12<003A>] TJ ET EMC /P <</Lang (zh-CN) /MCID 50 >> BDC BT 1 0 0 -1 0 42.85499954 Tm [<003B003C003D003E003F0040>] TJ ET EMC /P <</MCID 51 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 63 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 52 >> BDC BT 1 0 0 -1 68.25 42.85499954 Tm [(pH)] TJ ET EMC /P <</MCID 53 >> BDC BT 1 0 0 -1 81.08300018 42.85499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 54 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 86.33300018 42.85499954 Tm [<00410042004300440041001400450046004700160048>] TJ ET EMC /P <</MCID 55 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 201.83299255 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 56 >> BDC BT 1 0 0 -1 207.08299255 42.85499954 Tm [(pH)] TJ ET EMC /P <</MCID 57 >> BDC BT 1 0 0 -1 219.91600037 42.85499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 58 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 225.16600037 42.85499954 Tm [<0041002C0003000400050049>] TJ ET EMC /P <</MCID 59 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 288.16598511 42.85499954 Tm [(Tris)] TJ ET EMC /P <</MCID 60 >> BDC BT 1 0 0 -1 305.07998657 42.85499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 61 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 310.32998657 42.85499954 Tm [<004A0013004B004C004D0003000400050014004A>] TJ ET EMC /P <</Lang (zh-CN) /MCID 62 >> BDC BT 1 0 0 -1 0 58.45499802 Tm [<0013>] TJ ET EMC /P <</MCID 63 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 10.5 58.45499802 Tm [( )] TJ ET EMC /P <</MCID 64 >> BDC BT 1 0 0 -1 15.75 58.45499802 Tm [(pH)] TJ ET EMC /P <</MCID 65 >> BDC BT 1 0 0 -1 28.58300018 58.45499802 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 66 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 33.83300018 58.45499802 Tm [<0041001F>] TJ ET EMC /P <</MCID 67 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 54.83300018 58.45499802 Tm [( )] TJ ET EMC /P <</MCID 68 >> BDC BT 1 0 0 -1 60.08300018 58.45499802 Tm [(6.8)] TJ ET EMC /P <</Lang (zh-CN) /MCID 69 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 73.20800018 58.45499802 Tm [<004E>] TJ ET EMC /P <</MCID 70 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 83.70800018 58.45499802 Tm [(7.4)] TJ ET EMC /P <</Lang (zh-CN) /MCID 71 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 96.83300018 58.45499802 Tm [<004E>] TJ ET EMC /P <</MCID 72 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 107.33300018 58.45499802 Tm [(8.0)] TJ ET EMC /P <</Lang (zh-CN) /MCID 73 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 120.45800018 58.45499802 Tm [<004E>] TJ ET EMC /P <</MCID 74 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 130.95799255 58.45499802 Tm [(8.8)] TJ ET EMC /P <</Lang (zh-CN) /MCID 75 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 144.08299255 58.45499802 Tm [<0014004F>] TJ ET EMC /P <</MCID 76 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 165.08299255 58.45499802 Tm [( )] TJ ET EMC /P <</MCID 77 >> BDC BT 1 0 0 -1 170.33299255 58.45499802 Tm [(pH)] TJ ET EMC /P <</MCID 78 >> BDC BT 1 0 0 -1 183.16600037 58.45499802 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 79 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 188.41600037 58.45499802 Tm [<004100500051005200530054005500560049003800390057000600140051005200580059005A>] TJ ET EMC /P <</MCID 80 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 387.91598511 58.45499802 Tm [( )] TJ ET EMC /P <</MCID 81 >> BDC BT 1 0 0 -1 393.16598511 58.45499802 Tm [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 82 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 398.41598511 58.45499802 Tm [<00290014>] TJ ET EMC /P <</MCID 83 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 74.05500031 Tm [(pH)] TJ ET EMC /P <</Lang (zh-CN) /MCID 84 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 15.45800018 74.05500031 Tm [<0041002A005B>] TJ ET EMC /P <</MCID 85 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 46.95800018 74.05500031 Tm [( )] TJ ET EMC /P <</MCID 86 >> BDC BT 1 0 0 -1 52.20800018 74.05500031 Tm [(0.03)] TJ ET EMC /P <</Lang (zh-CN) /MCID 87 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 70.58300018 74.05500031 Tm [<0014>] TJ ET EMC /P <</MCID 88 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 81.08300018 74.05500031 Tm [(Tris)] TJ ET EMC /P <</MCID 89 >> BDC BT 1 0 0 -1 97.99700165 74.05500031 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 90 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 103.24700165 74.05500031 Tm [<0003000400050015000C005C005D005E0013004B005F003D0060006100620063002C0035006400140065002D00660067006800690013006A0049>] TJ ET EMC /P <</MCID 91 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 407.74700928 74.05500031 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 171.6000061 cm /P <</MCID 92 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 187.19999695 cm /P <</MCID 93 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(1M)] TJ ET EMC /P <</MCID 94 >> BDC BT 1 0 0 -1 14.58600044 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 95 >> BDC BT 1 0 0 -1 17.24699974 11.65499973 Tm [(Tris-HCl)] TJ ET EMC /P <</MCID 96 >> BDC BT 1 0 0 -1 55.1609993 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 97 >> BDC BT 1 0 0 -1 57.8219986 11.65499973 Tm [(pH6.)] TJ ET EMC /P <</MCID 98 >> BDC BT 1 0 0 -1 78.52999878 11.65499973 Tm [(8)] TJ ET EMC /P <</Lang (zh-CN) /MCID 99 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 86.44100189 11.65499973 Tm [<0060>] TJ ET EMC /P <</MCID 100 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 99.60199738 11.65499973 Tm [(1.5M)] TJ ET EMC /P <</MCID 101 >> BDC BT 1 0 0 -1 122.06300354 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 102 >> BDC BT 1 0 0 -1 124.72399902 11.65499973 Tm [(Tris-HCl)] TJ ET EMC /P <</MCID 103 >> BDC BT 1 0 0 -1 162.63800049 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 104 >> BDC BT 1 0 0 -1 165.29899597 11.65499973 Tm [(pH8.8)] TJ ET EMC /P <</MCID 105 >> BDC BT 1 0 0 -1 191.25700378 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 106 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 196.54299927 11.65499973 Tm [<006B>] TJ ET EMC /P <</MCID 107 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 207.07899475 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 108 >> BDC BT 1 0 0 -1 212.36500549 11.65499973 Tm [(SDS-P)-1(AGE)] TJ ET EMC /P <</MCID 109 >> BDC BT 1 0 0 -1 262.54199219 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 110 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 267.82800293 11.65499973 Tm [<006C>-3<006D>-4<006E>-3<004A>-4<0013>-3<002C>-4<006F>-3<0064>-3<0014>-4<0013>-3<0057>-4<0070>-3<0071>-4<0072>] TJ ET EMC /P <</Lang (zh-CN) /MCID 111 >> BDC BT 1 0 0 -1 0 27.25500107 Tm [<0073007400750076007700780049>] TJ ET EMC /P <</MCID 112 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 73.5 27.25500107 Tm [(Tris-HCl)] TJ ET EMC /P <</MCID 113 >> BDC BT 1 0 0 -1 111.41400146 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 114 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 116.66400146 27.25500107 Tm [<000300040005>] TJ ET EMC /P <</MCID 115 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 148.16400146 27.25500107 Tm [(\(1mol/L)1(,pH)-1(6.8\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 116 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 214.65499878 27.25500107 Tm [<00790013007A007B>] TJ ET EMC /P <</MCID 117 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 256.65499878 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 118 >> BDC BT 1 0 0 -1 261.90499878 27.25500107 Tm [(Tris)] TJ ET EMC /P <</Lang (zh-CN) /MCID 119 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 278.81900024 27.25500107 Tm [<004E007C007D0023003400700071007E007F0014004A00130057>] TJ ET EMC /P <</Lang (zh-CN) /MCID 120 >> BDC BT 1 0 0 -1 0 42.85499954 Tm [<00700071007200730074007500760077007800490048006F0064000C0013001700800010008100820014001500830013001700180019001A001B0084004F00850013006A0049>] TJ ET EMC /P <</MCID 121 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 367.5 42.85499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 234 cm /P <</MCID 122 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 249.6000061 cm /P <</Lang (zh-CN) /MCID 123 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<000A>-4<000B>-4<0086>-4<007F>-4<001E>] TJ ET EMC /P <</MCID 124 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 265.20001221 cm /P <</MCID 125 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 -10 280.79998779 cm q 0.25 0.5 m 137.75 0.5 l 137.75 26.10000038 l 0.25 26.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 126 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 45.52799988 16.10499954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<000A>-4<000B>-4<0087>-4<0088>] TJ ET EMC /P <</MCID 127 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 81.67199707 16.10499954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 138 0 cm 0.25 0.5 m 75.5 0.5 l 75.5 26.10000038 l 0.25 26.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 128 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 23.43899918 16.10499954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0089>-4<008A>] TJ ET EMC /P <</MCID 129 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 41.51100159 16.10499954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 213.75 0 cm 0.25 0.5 m 47.75 0.5 l 47.75 26.10000038 l 0.25 26.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 130 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 0.528 16.10499954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<008B>-4<008C>-4<008D>-4<008E>] TJ ET EMC /P <</MCID 131 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 36.67200089 16.10499954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 261.75 0 cm 0.25 0.5 m 46.25 0.5 l 46.25 26.10000038 l 0.25 26.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 132 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 4.296 16.10499954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0006>-4<0007>-4<0008>] TJ ET EMC /P <</MCID 133 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 31.40399933 16.10499954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 308.25 0 cm 0.25 0.5 m 38.75 0.5 l 38.75 26.10000038 l 0.25 26.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 134 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 0.546 16.10499954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<002D>-4<002E>-4<008F>] TJ ET EMC /P <</MCID 135 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 27.65399933 16.10499954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 26.10000038 cm q 0.25 0.5 m 137.75 0.5 l 137.75 26.10000038 l 0.25 26.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 136 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 4.22900009 16.10499954 Tm /GS2 gs 0 g [(Tris-HCl)] TJ ET EMC /P <</Lang (zh-CN) /MCID 137 >> BDC BT /FAAACB 9 Tf 1 0 0 -1 38.97700119 16.10499954 Tm [<000300040005>] TJ ET EMC /P <</MCID 138 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 65.97699738 16.10499954 Tm [(\(1mol/L)1(,pH)-1(6.8\))] TJ ET EMC /P <</MCID 139 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 122.97000122 16.10499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 138 0 cm 0.25 0.5 m 75.5 0.5 l 75.5 26.10000038 l 0.25 26.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 140 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 8.22399998 16.02700043 Tm /GS2 gs 0 g [(100ml/)1(500ml)] TJ ET EMC /P <</MCID 141 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 56.72600174 16.02700043 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 213.75 0 cm 0.25 0.5 m 47.75 0.5 l 47.75 26.10000038 l 0.25 26.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 142 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 12.85000038 16.02700043 Tm /GS2 gs 0 g [(RT)] TJ ET EMC /P <</MCID 143 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 24.35000038 16.02700043 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 261.75 0 cm 0.25 0.5 m 46.25 0.5 l 46.25 26.10000038 l 0.25 26.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 144 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 8.85000038 16.10499954 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</MCID 145 >> BDC BT 1 0 0 -1 13.35000038 16.10499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 146 >> BDC BT /FAAACB 9 Tf 1 0 0 -1 17.85000038 16.10499954 Tm [<0090>] TJ ET EMC /P <</MCID 147 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 26.85000038 16.10499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 308.25 0 cm 0.25 0.5 m 38.75 0.5 l 38.75 26.10000038 l 0.25 26.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 148 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 6.2249999 16.10499954 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 149 >> BDC BT /FAAACB 9 Tf 1 0 0 -1 12.97500038 16.10499954 Tm [<0091>] TJ ET EMC /P <</MCID 150 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 21.97500038 16.10499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0.5 w 0 0.5 m 0 52.20000076 l S Q q 0.5 w 138 0.5 m 138 26.10000038 l S Q q 0.5 w 138 26.60000038 m 138 52.20000076 l S Q q 0.5 w 213.75 0.5 m 213.75 26.10000038 l S Q q 0.5 w 213.75 26.60000038 m 213.75 52.20000076 l S Q q 0.5 w 261.75 0.5 m 261.75 26.10000038 l S Q q 0.5 w 261.75 26.60000038 m 261.75 52.20000076 l S Q q 0.5 w 308.25 0.5 m 308.25 26.10000038 l S Q q 0.5 w 308.25 26.60000038 m 308.25 52.20000076 l S Q q 0.5 w 347.25 0.5 m 347.25 52.20000076 l S Q q 0.5 w -0.25 0.25 m 347.5 0.25 l S Q q 0.5 w 0.25 26.35000038 m 347 26.35000038 l S Q q 0.5 w -0.25 52.45000076 m 347.5 52.45000076 l S Q Q q 1 0 0 1 0 333.5 cm /P <</MCID 151 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC /P <</MCID 152 >> BDC BT 1 0 0 -1 21 11.56599998 Tm [( )] TJ ET EMC /P <</MCID 153 >> BDC BT 1 0 0 -1 42 11.56599998 Tm [( )] TJ ET EMC /P <</MCID 154 >> BDC BT 1 0 0 -1 63 11.56599998 Tm [( )] TJ ET EMC /P <</MCID 155 >> BDC BT 1 0 0 -1 84 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 349.1000061 cm /P <</Lang (zh-CN) /MCID 156 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<0092>-4<004B>-4<0093>-4<0094>] TJ ET EMC /P <</MCID 157 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 42.16799927 11.65499973 Tm 0 Tr [(\()] TJ ET EMC /P <</Lang (zh-CN) /MCID 158 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 45.66500092 11.65499973 Tm 2 Tr [<000C>-4<000D>-4<0095>-4<0096>] TJ ET EMC /P <</MCID 159 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 87.83300018 11.65499973 Tm 0 Tr [(\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 160 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 91.33000183 11.65499973 Tm 2 Tr [<001E>] TJ ET EMC /P <</MCID 161 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 101.87200165 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 364.70001221 cm /P <</MCID 162 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 163 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<004E009700980099000E009A009B0069009C0092004B0049>] TJ ET EMC /P <</MCID 164 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 131.25 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 380.29998779 cm /P <</MCID 165 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 395.8999939 cm /P <</Lang (zh-CN) /MCID 166 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<009D>-4<009E>-4<009F>-4<00A0>-4<001E>] TJ ET EMC /P <</MCID 167 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 411.5 cm /P <</MCID 168 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 169 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<004E>] TJ ET EMC /P <</MCID 170 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 15.75 11.65499973 Tm [(Tris-HCl)] TJ ET EMC /P <</MCID 171 >> BDC BT 1 0 0 -1 53.66400146 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 172 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 58.91400146 11.65499973 Tm [<000300040005>] TJ ET EMC /P <</MCID 173 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 90.41400146 11.65499973 Tm [(\(1mol/L)1(,pH)-1(6.8\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 174 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 156.90499878 11.65499973 Tm [<002600A1000E002D00A200A300A4001400A5009D009E008300A600A700A80049>] TJ ET EMC /P <</MCID 175 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 324.90499878 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 427.1000061 cm /P <</MCID 176 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(2)] TJ ET EMC /P <</Lang (zh-CN) /MCID 177 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<004E001F00A900AA002C00AB00AC006000AD00AE001400A500AF009A009B00B000B100B2003800B300A400B400B50092004B0049>] TJ ET EMC /P <</MCID 178 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 278.25 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 442.70001221 cm /P <</MCID 179 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 458.29998779 cm /P <</Lang (zh-CN) /MCID 180 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<0025>-4<00B6>-4<000A>-4<000B>-4<001E>] TJ ET EMC /P <</MCID 181 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 473.8999939 cm /P <</MCID 182 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 -5.4000001 489.5 cm q 0.25 0.5 m 297.75 0.5 l 297.75 24.85000038 l 0.25 24.85000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 183 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(SDS-PAGE)] TJ ET EMC /P <</Lang (zh-CN) /MCID 184 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 44.625 11.65499973 Tm [<0022007D0077000300040005>] TJ ET EMC /P <</MCID 185 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 107.625 11.65499973 Tm [(\(4)] TJ ET EMC /P <</Lang (zh-CN) /MCID 186 >> BDC BT 1 0 0 -1 118.125 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 187 >> BDC BT 1 0 0 -1 128.625 11.65499973 Tm [(,pH8.8\))] TJ ET EMC /P <</MCID 188 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 165.375 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 24.85000038 cm q 0.25 0.5 m 297.75 0.5 l 297.75 24.85000038 l 0.25 24.85000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 189 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(SDS-PAGE)] TJ ET EMC /P <</Lang (zh-CN) /MCID 190 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 44.625 11.65499973 Tm [<00610062003A00C6000300040005>] TJ ET EMC /P <</MCID 191 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 118.125 11.65499973 Tm [(\(5)] TJ ET EMC /P <</Lang (zh-CN) /MCID 192 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 128.625 11.65499973 Tm [<00BF0014>] TJ ET EMC /P <</Lang (zh-CN) /MCID 193 >> BDC BT 1 0 0 -1 149.625 11.65499973 Tm [<00C8>] TJ ET EMC /P <</MCID 194 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 162.75 11.65499973 Tm [(TCEP\))] TJ ET EMC /P <</MCID 195 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 189 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 49.70000076 cm q 0.25 0.5 m 297.75 0.5 l 297.75 24.85000038 l 0.25 24.85000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 196 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(SDS-PAGE)] TJ ET EMC /P <</Lang (zh-CN) /MCID 197 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 44.625 11.65499973 Tm [<00610062003A00C6000300040005>] TJ ET EMC /P <</MCID 198 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 118.125 11.65499973 Tm [(\(5)] TJ ET EMC /P <</Lang (zh-CN) /MCID 199 >> BDC BT 1 0 0 -1 128.625 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 200 >> BDC BT 1 0 0 -1 139.125 11.65499973 Tm [(,)] TJ ET EMC /P <</Lang (zh-CN) /MCID 201 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 144.375 11.65499973 Tm [<00C8>] TJ ET EMC /P <</MCID 202 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 157.5 11.65499973 Tm [(DTT\))] TJ ET EMC /P <</MCID 203 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 178.5 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 74.55000305 cm q 0.25 0.5 m 297.75 0.5 l 297.75 24.85000038 l 0.25 24.85000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 204 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(SDS-PAGE)] TJ ET EMC /P <</Lang (zh-CN) /MCID 205 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 44.625 11.65499973 Tm [<00610062003A00C6000300040005>] TJ ET EMC /P <</MCID 206 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 118.125 11.65499973 Tm [(\(2)] TJ ET EMC /P <</Lang (zh-CN) /MCID 207 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 128.625 11.65499973 Tm [<00BF0014>] TJ ET EMC /P <</Lang (zh-CN) /MCID 208 >> BDC BT 1 0 0 -1 149.625 11.65499973 Tm [<00C8>] TJ ET EMC /P <</MCID 209 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 162.75 11.65499973 Tm [(TCEP\))] TJ ET EMC /P <</MCID 210 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 189 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0.5 w 0 0.5 m 0 99.40000153 l S Q q 0.5 w 298 0.5 m 298 99.40000153 l S Q q 0.5 w -0.25 0.25 m 298.25 0.25 l S Q q 0.5 w 0.25 25.10000038 m 297.75 25.10000038 l S Q q 0.5 w 0.25 49.95000076 m 297.75 49.95000076 l S Q q 0.5 w 0.25 74.80000305 m 297.75 74.80000305 l S Q q 0.5 w -0.25 99.65000153 m 298.25 99.65000153 l S Q Q q 1 0 0 1 0 589.40002441 cm /P <</MCID 211 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 605 cm Q Q 1 0 0 -1 0 841.90002441 cm 
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