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[( )] TJ ET EMC q 1 0 0 1 0 31.20000076 cm /P <</MCID 5 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 207.6499939 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 46.79999924 cm /P <</Lang (zh-CN) /MCID 6 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 102.23000336 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<0009>-4<000A>-4<000B>-4<000C>-4<000D>-4<000E>-3<000F>-4<0010>-4<0011>-4<0012>-4<0013>-4<0014>-4<0015>-4<0016>-4<0013>-4<0017>-3<0018>-4<0019>-4<001A>-4<001B>] TJ ET EMC /P <</MCID 7 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 313.07000732 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 62.40000153 cm /P <</MCID 8 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 78 cm /P <</Lang (zh-CN) /MCID 9 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<000A>-4<000B>-4<001C>-4<001D>-4<001E>] TJ ET EMC /P <</MCID 10 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 93.59999847 cm /P <</MCID 11 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris\()] TJ ET EMC /P <</Lang (zh-CN) /MCID 12 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 20.40999985 11.65499973 Tm [<001F0020002100220023002200210024>] TJ ET EMC /P <</MCID 13 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 104.41000366 11.65499973 Tm [(\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 14 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 107.90699768 11.65499973 Tm [<002500260027001400280029002A0025>] TJ ET EMC /P <</MCID 15 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 191.90699768 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 16 >> BDC BT 1 0 0 -1 197.15699768 11.65499973 Tm [(C4H11NO3)] TJ ET EMC /P <</Lang (zh-CN) /MCID 17 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 247.90899658 11.65499973 Tm [<0014002B002C00280029002D0025>] TJ ET EMC /P <</MCID 18 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 321.40899658 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 19 >> BDC BT 1 0 0 -1 326.65899658 11.65499973 Tm [(121.14,)] TJ ET EMC /P <</MCID 20 >> BDC BT 1 0 0 -1 358.15899658 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 21 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 363.40899658 11.65499973 Tm [<002E>] TJ ET EMC /P <</MCID 22 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 373.90899658 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 23 >> BDC BT 1 0 0 -1 379.15899658 11.65499973 Tm [(25)] TJ ET EMC /P <</Lang (zh-CN) /MCID 24 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 389.65899658 11.65499973 Tm [<002F>] TJ ET EMC /P <</MCID 25 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 400.15899658 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 26 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 405.40899658 11.65499973 Tm [<0030>] TJ ET EMC /P <</MCID 27 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 415.90899658 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 28 >> BDC BT 1 0 0 -1 0 27.25500107 Tm [(pKa)] TJ ET EMC /P <</MCID 29 >> BDC BT 1 0 0 -1 17.49300003 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 30 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 22.74300003 27.25500107 Tm [<0025>] TJ ET EMC /P <</MCID 31 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 33.24300003 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 32 >> BDC BT 1 0 0 -1 38.49300003 27.25500107 Tm [(8.1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 33 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 51.61800003 27.25500107 Tm [<0014>] TJ ET EMC /P <</MCID 34 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 62.11800003 27.25500107 Tm [(Tris)] TJ ET EMC /P <</MCID 35 >> BDC BT 1 0 0 -1 79.03199768 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 36 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 84.28199768 27.25500107 Tm [<0003000400050031003200330003000400340035002E>] TJ ET EMC /P <</MCID 37 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 199.78199768 27.25500107 Tm [( )] TJ ET EMC /P <</MCID 38 >> BDC BT 1 0 0 -1 205.03199768 27.25500107 Tm [(pH7.0)] TJ ET EMC /P <</Lang (zh-CN) /MCID 39 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 230.99000549 27.25500107 Tm [<0036>] TJ ET EMC /P <</MCID 40 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 241.49000549 27.25500107 Tm [(9.2)] TJ ET EMC /P <</MCID 41 >> BDC BT 1 0 0 -1 254.61500549 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 42 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 259.86499023 27.25500107 Tm [<00370038001400130039003A0025003B003C00030004000500310022003D>] TJ ET EMC /P <</Lang (zh-CN) /MCID 43 >> BDC BT 1 0 0 -1 0 42.85499954 Tm [<003E00280014003F00400041002700420043>] TJ ET EMC /P <</MCID 44 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 94.5 42.85499954 Tm [(Tris-Trit)1(on-N)-1(a)1(Cl)] TJ ET EMC /P <</MCID 45 >> BDC BT 1 0 0 -1 166.81599426 42.85499954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 46 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 172.06599426 42.85499954 Tm [<000300040005>] TJ ET EMC /P <</MCID 47 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 203.56599426 42.85499954 Tm [(\(pH8.0\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 48 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 236.51699829 42.85499954 Tm [<0044>] TJ ET EMC /P <</MCID 49 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 247.01699829 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 50 >> BDC BT 1 0 0 -1 252.26699829 42.85499954 Tm [(50mM)] TJ ET EMC /P <</MCID 51 >> BDC BT 1 0 0 -1 280.26998901 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 52 >> BDC BT 1 0 0 -1 282.89498901 42.85499954 Tm [(Tris-HCl)] TJ ET EMC /P <</MCID 53 >> BDC BT 1 0 0 -1 320.80899048 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 54 >> BDC BT 1 0 0 -1 323.43399048 42.85499954 Tm [(\(pH8.0\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 55 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 356.38500977 42.85499954 Tm [<0045>] TJ ET EMC /P <</MCID 56 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 366.88500977 42.85499954 Tm [(0.1%)] TJ ET EMC /P <</MCID 57 >> BDC BT 1 0 0 -1 388.75698853 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 58 >> BDC BT 1 0 0 -1 391.38198853 42.85499954 Tm [(Triton)] TJ ET EMC /P <</MCID 59 >> BDC BT 1 0 0 -1 417.62701416 42.85499954 Tm [( )] TJ ET EMC /P <</MCID 60 >> BDC BT 1 0 0 -1 0 58.45499802 Tm [(X-100)] TJ ET EMC /P <</Lang (zh-CN) /MCID 61 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 26.82900047 58.45499802 Tm [<00450046004700480049003E001400130017004A004B004C004D004E004F005000510031005200290043>] TJ ET EMC /P <</MCID 62 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 247.32899475 58.45499802 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 156 cm /P <</MCID 63 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 171.6000061 cm /P <</Lang (zh-CN) /MCID 64 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<000A>-4<000B>-4<0049>-4<003E>-4<001E>] TJ ET EMC /P <</MCID 65 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 187.19999695 cm /P <</MCID 66 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 -10 202.80000305 cm q 0.25 0.5 m 147.5 0.5 l 147.5 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 67 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 50.40299988 16.22999954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<000A>-4<000B>-4<0053>-4<0054>] TJ ET EMC /P <</MCID 68 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 86.54699707 16.22999954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 147.75 0 cm 0.25 0.5 m 52.25 0.5 l 52.25 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 69 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 11.81400013 16.22999954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0055>-4<0056>] TJ ET EMC /P <</MCID 70 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 29.88599968 16.22999954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 200.25 0 cm 0.25 0.5 m 50 0.5 l 50 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 71 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 1.653 16.22999954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0057>-4<0058>-4<0059>-4<005A>] TJ ET EMC /P <</MCID 72 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 37.79700089 16.22999954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 250.5 0 cm 0.25 0.5 m 50 0.5 l 50 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 73 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 6.171 16.22999954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0006>-4<0007>-4<0008>] TJ ET EMC /P <</MCID 74 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 33.27899933 16.22999954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 300.75 0 cm 0.25 0.5 m 43 0.5 l 43 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 75 >> BDC BT /FAAABH 9 Tf 1 0 0 -1 2.671 16.22999954 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0032>-4<0033>-4<005B>] TJ ET EMC /P <</MCID 76 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 29.77899933 16.22999954 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 26.35000038 cm q 0.25 0.5 m 147.5 0.5 l 147.5 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 77 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 7.61000013 16.22999954 Tm /GS2 gs 0 g [(Tris-Trit)1(on-N)-1(a)1(Cl)] TJ ET EMC /P <</MCID 78 >> BDC BT 1 0 0 -1 69.59500122 16.22999954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 79 >> BDC BT /FAAACB 9 Tf 1 0 0 -1 74.09500122 16.22999954 Tm [<000300040005>] TJ ET EMC /P <</MCID 80 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 101.09500122 16.22999954 Tm [(\(pH8.0\))] TJ ET EMC /P <</MCID 81 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 129.33900452 16.22999954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 147.75 0 cm 0.25 0.5 m 52.25 0.5 l 52.25 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 82 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 9.34899998 16.15200043 Tm /GS2 gs 0 g [(500ml)] TJ ET EMC /P <</MCID 83 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 32.34999847 16.15200043 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 200.25 0 cm 0.25 0.5 m 50 0.5 l 50 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 84 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 13.97500038 16.15200043 Tm /GS2 gs 0 g [(RT)] TJ ET EMC /P <</MCID 85 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 25.47500038 16.15200043 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 250.5 0 cm 0.25 0.5 m 50 0.5 l 50 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 86 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 10.72500038 16.22999954 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</MCID 87 >> BDC BT 1 0 0 -1 15.22500038 16.22999954 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 88 >> BDC BT /FAAACB 9 Tf 1 0 0 -1 19.72500038 16.22999954 Tm [<005C>] TJ ET EMC /P <</MCID 89 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 28.72500038 16.22999954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 300.75 0 cm 0.25 0.5 m 43 0.5 l 43 26.35000038 l 0.25 26.35000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 90 >> BDC BT /FAAAAI 9 Tf 1 0 0 -1 8.35000038 16.22999954 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 91 >> BDC BT /FAAACB 9 Tf 1 0 0 -1 15.10000038 16.22999954 Tm [<005D>] TJ ET EMC /P <</MCID 92 >> BDC BT /FAAABD 9 Tf 1 0 0 -1 24.10000038 16.22999954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0.5 w 0 0.5 m 0 52.70000076 l S Q q 0.5 w 147.75 0.5 m 147.75 26.35000038 l S Q q 0.5 w 147.75 26.85000038 m 147.75 52.70000076 l S Q q 0.5 w 200.25 0.5 m 200.25 26.35000038 l S Q q 0.5 w 200.25 26.85000038 m 200.25 52.70000076 l S Q q 0.5 w 250.5 0.5 m 250.5 26.35000038 l S Q q 0.5 w 250.5 26.85000038 m 250.5 52.70000076 l S Q q 0.5 w 300.75 0.5 m 300.75 26.35000038 l S Q q 0.5 w 300.75 26.85000038 m 300.75 52.70000076 l S Q q 0.5 w 344 0.5 m 344 52.70000076 l S Q q 0.5 w -0.25 0.25 m 344.25 0.25 l S Q q 0.5 w 0.25 26.60000038 m 343.75 26.60000038 l S Q q 0.5 w -0.25 52.95000076 m 344.25 52.95000076 l S Q Q q 1 0 0 1 0 256 cm /P <</MCID 93 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 271.6000061 cm /P <</Lang (zh-CN) /MCID 94 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<005E>-4<003A>-4<005F>-4<0060>] TJ ET EMC /P <</MCID 95 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 42.16799927 11.65499973 Tm 0 Tr [(\()] TJ ET EMC /P <</Lang (zh-CN) /MCID 96 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 45.66500092 11.65499973 Tm 2 Tr [<000C>-4<000D>-4<0061>-4<0062>] TJ ET EMC /P <</MCID 97 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 87.83300018 11.65499973 Tm 0 Tr [(\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 98 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 91.33000183 11.65499973 Tm 2 Tr [<001E>] TJ ET EMC /P <</MCID 99 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 101.87200165 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 287.20001221 cm /P <</MCID 100 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 101 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<00450063006400650066000E00670068005E003A00140069006A006B006C0014006D006E001200130043>] TJ ET EMC /P <</MCID 102 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 225.75 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 302.79998779 cm /P <</MCID 103 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 318.3999939 cm /P <</Lang (zh-CN) /MCID 104 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<006F>-4<0070>-4<0071>-4<0072>-4<001E>] TJ ET EMC /P <</MCID 105 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 334 cm /P <</MCID 106 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 107 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<0045002500730074003100750076004F0077007800140079007A00640065007B007C007D007E007F008000810082005E003A0043>] TJ ET EMC /P <</MCID 108 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 278.25 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 349.6000061 cm /P <</MCID 109 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 365.20001221 cm /P <</Lang (zh-CN) /MCID 110 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<002B>-4<0083>-4<000A>-4<000B>-4<001E>] TJ ET EMC /P <</MCID 111 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 380.79998779 cm /P <</MCID 112 >> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 0 11.56599998 Tm [( )] TJ ET EMC Q q 1 0 0 1 -5.4000001 396.3999939 cm q 0.25 0.5 m 297.75 0.5 l 297.75 25.14999962 l 0.25 25.14999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 113 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris-)] TJ ET EMC /P <</Lang (zh-CN) /MCID 114 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 26.25 11.65499973 Tm [<008900230041008A008B008C008D>] TJ ET EMC /P <</MCID 115 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 99.75 11.65499973 Tm [(\(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 116 >> BDC BT 1 0 0 -1 110.25 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 117 >> BDC BT 1 0 0 -1 120.75 11.65499973 Tm [(\))] TJ ET EMC /P <</MCID 118 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 126 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 25.14999962 cm q 0.25 0.5 m 297.75 0.5 l 297.75 25.14999962 l 0.25 25.14999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 119 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris-Tricine)] TJ ET EMC /P <</Lang (zh-CN) /MCID 120 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 65.625 11.65499973 Tm [<00950096000300040005>] TJ ET EMC /P <</MCID 121 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 118.125 11.65499973 Tm [(\(5)] TJ ET EMC /P <</Lang (zh-CN) /MCID 122 >> BDC BT 1 0 0 -1 128.625 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 123 >> BDC BT 1 0 0 -1 139.125 11.65499973 Tm [(\))] TJ ET EMC /P <</MCID 124 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 144.375 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 50.29999924 cm q 0.25 0.5 m 297.75 0.5 l 297.75 25.14999962 l 0.25 25.14999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 125 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris-Tricine)] TJ ET EMC /P <</Lang (zh-CN) /MCID 126 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 65.625 11.65499973 Tm [<00980096000300040005>] TJ ET EMC /P <</MCID 127 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 118.125 11.65499973 Tm [(\(5)] TJ ET EMC /P <</Lang (zh-CN) /MCID 128 >> BDC BT 1 0 0 -1 128.625 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 129 >> BDC BT 1 0 0 -1 139.125 11.65499973 Tm [(,pH8.9\))] TJ ET EMC /P <</MCID 130 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 175.875 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 75.44999695 cm q 0.25 0.5 m 297.75 0.5 l 297.75 25.14999962 l 0.25 25.14999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 131 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris-MOPS-SDS)] TJ ET EMC /P <</Lang (zh-CN) /MCID 132 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 70.875 11.65499973 Tm [<008A008B000300040005>] TJ ET EMC /P <</MCID 133 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 123.375 11.65499973 Tm [(\(20)] TJ ET EMC /P <</Lang (zh-CN) /MCID 134 >> BDC BT 1 0 0 -1 139.125 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 135 >> BDC BT 1 0 0 -1 149.625 11.65499973 Tm [(,pH8.3\))] TJ ET EMC /P <</MCID 136 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 186.375 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 100.59999847 cm q 0.25 0.5 m 297.75 0.5 l 297.75 25.14999962 l 0.25 25.14999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 137 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(Tris-MOPS-SDS)] TJ ET EMC /P <</Lang (zh-CN) /MCID 138 >> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 70.875 11.65499973 Tm [<008A008B008C008D>] TJ ET EMC /P <</MCID 139 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 112.875 11.65499973 Tm [(\(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 140 >> BDC BT 1 0 0 -1 123.375 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 141 >> BDC BT 1 0 0 -1 133.875 11.65499973 Tm [(\))] TJ ET EMC /P <</MCID 142 >> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 139.125 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0.5 w 0 0.5 m 0 125.75 l S Q q 0.5 w 298 0.5 m 298 125.75 l S Q q 0.5 w -0.25 0.25 m 298.25 0.25 l S Q q 0.5 w 0.25 25.39999962 m 297.75 25.39999962 l S Q q 0.5 w 0.25 50.54999924 m 297.75 50.54999924 l S Q q 0.5 w 0.25 75.69999695 m 297.75 75.69999695 l S Q q 0.5 w 0.25 100.84999847 m 297.75 100.84999847 l S Q q 0.5 w -0.25 126 m 298.25 126 l S Q Q q 1 0 0 1 0 522.65002441 cm Q Q 1 0 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