%PDF-1.7 %ÈÈÈÈÈÈ?1 0 obj <> endobj 2 0 obj <>/Metadata 116 0 R>> endobj 3 0 obj <> endobj 4 0 obj <>/ExtGState<>/XObject<>>>/Group<>/StructParents 0/Tabs/S>> endobj 5 0 obj <>stream 1 0 0 -1 0 841.90002441 cm q 1 g 0 0 m 600 0 l 600 450 l 0 450 l h f* Q q 1 0 0 1 0 450 cm q 1 g 0 0 m 600 0 l 600 450 l 0 450 l h f* Q Q q 1 0 0 1 65.65000153 94.18599701 cm q 474.8500061 0 0 -20.79999924 0 20.79999924 cm /X1 Do Q Q /Artifact <> BDC q 1 0 0 1 90 42.54999924 cm -90 0 m 505.29998779 0 l 505.29998779 87.3690033 l -90 87.3690033 l h W n BT /FAAAAI 9 Tf 1 0 0 -1 0 9.56900024 Tm 0 g [( )-1( )-1( )-1( )] TJ /GS1 gs 0 g [-11231( )] TJ ET q 1 0 0 1 0 11.98600006 cm BT 1 0 0 -1 0 41.42499924 Tm /GS2 gs 0 g [( )-1( )-1( )-1( )-1( )] TJ 1 0 0 -1 413.36099243 41.42499924 Tm /GS1 gs 0 g [( )] TJ ET Q q 1 0 0 1 0 53.4109993 cm BT 1 0 0 -1 0 8.56900024 Tm /GS2 gs 0 g [( )-1( )-1( )] TJ /GS1 gs 0 g [-9040( )] TJ ET Q q 1 0 0 1 0 64.39700317 cm BT 1 0 0 -1 0 8.56900024 Tm [( )] TJ ET Q q 1 0 0 1 0 75.38300323 cm BT 1 0 0 -1 0 8.56900024 Tm /GS2 gs 0 g [( )] TJ /FAAABD 9 Tf [-548( )] TJ ET Q Q EMC q 1 0 0 1 90 54.5359993 cm q 1 0 0 1 225 -0.00000076 cm q 188.3614502 0 0 -41.42535019 0 41.42535019 cm /X2 Do Q Q Q q 1 0 0 1 90 129.91900635 cm /P <> BDC BT /FAAABD 16 Tf 1 0 0 -1 0 21.06800079 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 54.8769989 Tm /GS2 gs 0 g [( )] TJ ET EMC /P <> BDC BT /FAAABD 22 Tf 1 0 0 -1 56 54.8769989 Tm [(HEPES)1(-BSA)] TJ ET EMC /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 161.7460022 54.8769989 Tm 2 Tr 0.62857145 w 0 G [<0003>-4<0004>] TJ ET EMC /P <> BDC BT /FAAABD 22 Tf 1 0 0 -1 205.92199707 54.8769989 Tm 0 Tr [(\(H)1(A)] TJ ET EMC /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 245.48500061 54.8769989 Tm 2 Tr [<0003>-4<0004>] TJ ET EMC /P <> BDC BT /FAAABD 22 Tf 1 0 0 -1 289.66101074 54.8769989 Tm 0 Tr [(\))] TJ ET EMC /P <> BDC BT /FAAABH 22 Tf 1 0 0 -1 296.51501465 54.8769989 Tm 2 Tr [<0005>-4<0006>-4<0007>] TJ ET EMC /P <> BDC BT /FAAABD 16 Tf 1 0 0 -1 362.7789917 54.8769989 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC q 1 0 0 1 69.80000305 62.40000153 cm /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 0 11.38799953 Tm /GS2 gs 0 g [( )-1( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 42 11.38799953 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 10.5 27.25500107 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<0008>-4<0009>-4<000A>-4<000B>-4<000C>-4<000D>-3<000E>-4<000F>-4<0010>-4<0011>-4<0012>-4<0013>-4<0014>-4<0015>-4<0012>-4<0016>-3<0017>-4<0018>-4<0019>-4<001A>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 221.33999634 27.25500107 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 93.59999847 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.38799953 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 109.19999695 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.38799953 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 124.80000305 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<0009>-4<000A>-4<001B>-4<001C>-4<001D>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 140.3999939 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(HEPES)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 30.10099983 11.65499973 Tm [<001E>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 43.6570015 11.65499973 Tm [(4-\(2-Hydroxyethyl\)p)-1(ip)-1(e)1(razin)-1(e)1(-1-ethan)-1(e)1(su)-1(lfo)-1(ni)-1(c)] TJ ET EMC /P <> BDC BT 1 0 0 -1 243.40299988 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 246.20799255 11.65499973 Tm [(acid)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 264.03500366 11.65499973 Tm [<0013>-41<001F>-41<0020>-41<0021>-41<0022>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 318.69000244 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 324.3710022 11.65499973 Tm [(N-\(2-)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 346.51501465 11.65499973 Tm [<0023>-41<0024>-41<0025>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 379.30801392 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 384.98901367 11.65499973 Tm [(\))] TJ ET EMC /P <> BDC BT 1 0 0 -1 388.17300415 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 393.85400391 11.65499973 Tm [<0026>-41<0027>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 415.71600342 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [(-N')1(-)-1(2)] TJ ET EMC /P <> BDC BT 1 0 0 -1 20.84600067 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 26.09600067 27.25500107 Tm [<002800290013002A002B002C002D>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 99.59600067 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 104.84600067 27.25500107 Tm [(C8H18N)1(2O4S)] TJ ET EMC /P <> BDC BT 1 0 0 -1 162.1499939 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 167.3999939 27.25500107 Tm [<0013002A002B002E002D>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 219.8999939 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 225.1499939 27.25500107 Tm [(238.31)] TJ ET EMC /P <> BDC BT 1 0 0 -1 254.41000366 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 259.66000366 27.25500107 Tm [<0013>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 270.16000366 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 275.41000366 27.25500107 Tm [(CAS)] TJ ET EMC /P <> BDC BT 1 0 0 -1 291.90899658 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 294.2829895 27.25500107 Tm [(Number)] TJ ET EMC /P <> BDC BT 1 0 0 -1 329.36700439 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 331.74099731 27.25500107 Tm [(7365)1(-45-9)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 375.42300415 27.25500107 Tm [<0013002F00300031>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 42.85499954 Tm [<0032>-59<0033>-59<0034>-59<0035>-58<0012>-59<0013>-59<0036>-59<0037>-59<0038>-59<0039>-59<003A>-58<002B>-59<003B>-59<0034>-59<003C>-59<003D>-59<003E>-59<0013>-58<003F>-59<0040>-59<0041>-59<0042>-59<0043>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 255.71400452 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 261.58200073 42.85499954 Tm [(pH)] TJ ET EMC /P <> BDC BT 1 0 0 -1 273.64099121 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 279.50900269 42.85499954 Tm [<0044>-59<0045>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 301.74499512 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 307.61300659 42.85499954 Tm [(6.8)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 321.5249939 42.85499954 Tm [<0046>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 332.64300537 42.85499954 Tm [(8.)] TJ ET EMC /P <> BDC BT 1 0 0 -1 340.61499023 42.85499954 Tm [(2)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 349.17999268 42.85499954 Tm [<0047>-59<0048>-59<0013>-59<0049>-58<004A>-59<0045>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 415.88800049 42.85499954 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 58.45499802 Tm [(pH7.)-1(2)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 25.35300064 58.45499802 Tm [<0046>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 35.85300064 58.45499802 Tm [(7.4)] TJ ET EMC /P <> BDC BT 1 0 0 -1 49.14699936 58.45499802 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 54.39699936 58.45499802 Tm [<004B0040004C004D004E003C003D003F004F>] TJ ET EMC /P <> BDC BT 1 0 0 -1 148.89700317 58.45499802 Tm [<0013>] TJ ET EMC /P <> BDC BT 1 0 0 -1 155.10600281 58.45499802 Tm [<00500051005200370053002D>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 218.10600281 58.45499802 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 223.35600281 58.45499802 Tm [(10)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 234 58.45499802 Tm [<0046>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 244.5 58.45499802 Tm [(50mm)1(o)-1(l/L)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 288.33499146 58.45499802 Tm [<0013>] TJ ET EMC /P <> BDC BT 1 0 0 -1 294.54400635 58.45499802 Tm [<00540055000400560057>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 347.04400635 58.45499802 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 352.29400635 58.45499802 Tm [(20mm)1(o)-1(l/L)-1(HEPES)] TJ ET EMC /P <> BDC BT 1 0 0 -1 423.17401123 58.45499802 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 0 74.05500031 Tm [<001E00580059005A004C004D004E003C003D003F004F0013005B005C00360045005D005E002C00540055005F00300031006000610062003F00630064004C00650066>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 346.5 74.05500031 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 351.75 74.05500031 Tm [(pH)] TJ ET EMC /P <> BDC BT 1 0 0 -1 363.80899048 74.05500031 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 369.05899048 74.05500031 Tm [<00440067>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 390.05899048 74.05500031 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 218.3999939 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.38799953 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 234 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(HEPES)-1(-BSA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 46.87099838 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 53.10400009 11.65499973 Tm [<0003>-94<0004>-93<001B>-94<0022>-93<002D>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 110.51899719 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 116.7519989 11.65499973 Tm [(HA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 129.3690033 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 135.602005 11.65499973 Tm [<0003>-94<0004>-93<0013>-94<0068>-93<0016>-94<0030>-94<0031>-93<0054>-94<0055>-94<0004>-93<004E>-94<0069>-93<002A>-94<006A>-94<0037>-93<0013>-94<004A>-94<001F>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 342.29598999 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 348.5289917 11.65499973 Tm [(HEPES)] TJ ET EMC /P <> BDC BT 1 0 0 -1 375.57400513 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 381.80700684 11.65499973 Tm [<0051>-94<0052>-93<002D>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 416.25601196 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 27.25500107 Tm [(20mm)1(o)-1(l/L)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 43.83499908 27.25500107 Tm [<0013>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 54.33499908 27.25500107 Tm [(BSA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 70.94599915 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 76.19599915 27.25500107 Tm [<00510052002D>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 107.69599915 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 112.94599915 27.25500107 Tm [(0.025%)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 144.38999939 27.25500107 Tm [<001300140057>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 175.88999939 27.25500107 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 181.13999939 27.25500107 Tm [(Ca2+)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 202.31900024 27.25500107 Tm [<006B>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 212.81900024 27.25500107 Tm [(Mg2+)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 237.28999329 27.25500107 Tm [<00130057006C002A004A006D006E003A002B0013006F007000710072007300740075>] TJ ET EMC /P <> BDC BT 1 0 0 -1 415.79000854 27.25500107 Tm [<0013>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 42.85499954 Tm [<00760077001200160078007900300031007A007B00540055001F004E007C007D>] TJ ET EMC /P <> BDC BT 1 0 0 -1 168 42.85499954 Tm [<006B>] TJ ET EMC /P <> BDC BT 1 0 0 -1 176.93400574 42.85499954 Tm [<00540055>] TJ ET EMC /P <> BDC BT 1 0 0 -1 197.93400574 42.85499954 Tm [<0067>] TJ ET EMC /P <> BDC BT 1 0 0 -1 206.86799622 42.85499954 Tm [<007E007F003E000B001200160080000F00810082>] TJ ET EMC /P <> BDC BT 1 0 0 -1 311.86801147 42.85499954 Tm [<0013>] TJ ET EMC /P <> BDC BT 1 0 0 -1 320.80200195 42.85499954 Tm [<0014007700120016001700180019001A005F>] TJ ET EMC /P <> BDC BT 1 0 0 -1 0 58.45499802 Tm [<004A006D001200830067>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 52.5 58.45499802 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 296.3999939 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.38799953 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 312 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<0009>-4<000A>-4<0078>-4<0069>-4<001D>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 327.6000061 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.38799953 Tm [( )] TJ ET EMC Q q 1 0 0 1 -5.4000001 343.20001221 cm q 0.25 0.5 m 123.34999847 0.5 l 123.34999847 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAABH 9 Tf 1 0 0 -1 38.32799911 15.00499916 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0009>-4<000A>-4<0021>-4<0022>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 74.47200012 14.77600098 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 123.59999847 0 cm 0.25 0.5 m 76.5 0.5 l 76.5 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAABH 9 Tf 1 0 0 -1 23.93899918 15.00499916 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0084>-4<0085>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 42.01100159 14.77600098 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 200.3500061 0 cm 0.25 0.5 m 74.30000305 0.5 l 74.30000305 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAABH 9 Tf 1 0 0 -1 13.80300045 15.00499916 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0086>-4<0087>-4<0088>-4<0089>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 49.9469986 14.77600098 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 274.8999939 0 cm 0.25 0.5 m 50.15000153 0.5 l 50.15000153 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAABH 9 Tf 1 0 0 -1 6.24599981 15.00500011 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0005>-4<0006>-4<0007>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 33.35400009 15.00500011 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 325.29998779 0 cm 0.25 0.5 m 49.45000076 0.5 l 49.45000076 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAABH 9 Tf 1 0 0 -1 5.89599991 15.00500011 Tm 2 Tr /GS2 gs 0 g 0.25714287 w [<0040>-4<0041>-4<008A>] TJ ET EMC /P <> BDC BT /FAAABD 9 Tf 1 0 0 -1 33.00400162 15.00500011 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 23.89999962 cm q 0.25 0.5 m 123.34999847 0.5 l 123.34999847 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 7.92600012 14.77600098 Tm /GS2 gs 0 g [(HEPES)-1(-BSA)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 50.35100174 15.00499916 Tm [<00030004>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 68.35099792 14.77600098 Tm [(\(HA)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 84.14499664 15.00499916 Tm [<00030004>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 102.14499664 14.77600098 Tm [(\))] TJ ET EMC /P <> BDC BT 1 0 0 -1 104.87400055 14.77600098 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 123.59999847 0 cm 0.25 0.5 m 76.5 0.5 l 76.5 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACF 9 Tf 1 0 0 -1 21.72500038 15.00499916 Tm /GS2 gs 0 g [(100ml)] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 44.22499847 14.77600098 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 200.3500061 0 cm 0.25 0.5 m 74.30000305 0.5 l 74.30000305 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACF 9 Tf 1 0 0 -1 27.375 15.00499916 Tm /GS2 gs 0 g [(RT)] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 36.375 14.77600098 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 274.8999939 0 cm 0.25 0.5 m 50.15000153 0.5 l 50.15000153 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 11.89400005 15.00500011 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 18.70599937 15.00500011 Tm [<0091>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 27.70599937 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 325.29998779 0 cm 0.25 0.5 m 49.45000076 0.5 l 49.45000076 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 11.54399967 15.00500011 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 9 Tf 1 0 0 -1 18.3560009 15.00500011 Tm [<0092>] TJ ET EMC /P <> BDC BT /FAAAAI 9 Tf 1 0 0 -1 27.3560009 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0.5 w 0 0.5 m 0 47.79999924 l S Q q 0.5 w 123.59999847 0.5 m 123.59999847 23.89999962 l S Q q 0.5 w 123.59999847 24.39999962 m 123.59999847 47.79999924 l S Q q 0.5 w 200.3500061 0.5 m 200.3500061 23.89999962 l S Q q 0.5 w 200.3500061 24.39999962 m 200.3500061 47.79999924 l S Q q 0.5 w 274.90002441 0.5 m 274.90002441 23.89999962 l S Q q 0.5 w 274.90002441 24.39999962 m 274.90002441 47.79999924 l S Q q 0.5 w 325.29998779 0.5 m 325.29998779 23.89999962 l S Q q 0.5 w 325.29998779 24.39999962 m 325.29998779 47.79999924 l S Q q 0.5 w 375 0.5 m 375 47.79999924 l S Q q 0.5 w -0.25 0.25 m 375.25 0.25 l S Q q 0.5 w 0.25 24.14999962 m 374.75 24.14999962 l S Q q 0.5 w -0.25 48.04999924 m 375.25 48.04999924 l S Q Q q 1 0 0 1 0 391.5 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.38799953 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 21 11.38799953 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 42 11.38799953 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 63 11.38799953 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 407.1000061 cm /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 0 11.38799953 Tm [( )] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 27.25500107 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<0093>-4<0035>-4<0094>-4<0095>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 42.16799927 27.25500107 Tm 0 Tr [(\()] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 45.43899918 27.25500107 Tm 2 Tr [<000B>-4<000C>-4<0096>-4<0097>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 87.60700226 27.25500107 Tm 0 Tr [(\))] TJ ET EMC /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 90.87799835 27.25500107 Tm 2 Tr [<001D>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 101.41999817 27.25500107 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 438.29998779 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.32200003 11.65499973 Tm [<006B0098009900780079009A009B009C0067>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 99.8219986 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 453.8999939 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(2)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.32200003 11.65499973 Tm [<006B009D009E00300031009F00700071001300A00099007100040013>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 152.32200623 11.65499973 Tm [(4)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 157.64399719 11.65499973 Tm [<00A1>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 168.14399719 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 173.39399719 11.65499973 Tm [(400g)] TJ ET EMC /P <> BDC BT 1 0 0 -1 194.30200195 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 199.55200195 11.65499973 Tm [<003A00A2>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 220.55200195 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 225.80200195 11.65499973 Tm [(10min)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 252.75999451 11.65499973 Tm [<001300A300A400A5007C0067>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 315.76000977 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 469.5 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(3)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.32200003 11.65499973 Tm [<006B0012>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 26.3220005 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT 1 0 0 -1 31.5720005 11.65499973 Tm [(HEPES)-1(-BSA)] TJ ET EMC /P <> BDC BT 1 0 0 -1 78.44300079 11.65499973 Tm [( )] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 83.69300079 11.65499973 Tm [<0003000400A600A700A800A9003000310013>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 178.19299316 11.65499973 Tm [(4)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 183.51499939 11.65499973 Tm [<00A100860087001300AA00AB00AC00AD00AE00AF00B000B100B20067>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 330.51501465 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 485.1000061 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.38799953 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 500.70001221 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<00B3>-4<00B4>-4<00B5>-4<00B6>-4<001D>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 516.29998779 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(1)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.32200003 11.65499973 Tm [<006B00B300B40032007300930035001300B7002E00B800B900BA00BB0067>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 162.82200623 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 531.90002441 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [(2)] TJ ET EMC /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 5.32200003 11.65499973 Tm [<006B002D00BC00BD004E00BE00BF00C000C100C2001300C300C400B100B200C5009A00C6003700C7003400C800C9009300350067>] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 278.32199097 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 547.5 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.38799953 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 563.09997559 cm /P <> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr /GS2 gs 0 g 0.30000001 w [<00CA>-4<00CB>-4<0009>-4<000A>-4<001D>] TJ ET EMC /P <> BDC BT /FAAABD 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 0 Tr /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 578.70001221 cm /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 0 11.38799953 Tm [( )] TJ ET EMC Q q 1 0 0 1 -5.4000001 594.29998779 cm q 0.25 0.5 m 364.75 0.5 l 364.75 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [<00CC002900CD00CE00030004>] TJ ET EMC /P <> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 63 11.65499973 Tm [(\(3.7%\))] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 94.5 11.38799953 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 16.10000038 cm q 0.25 0.5 m 364.75 0.5 l 364.75 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <> BDC BT /FAAACB 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS2 gs 0 g [<00D500D6002500D7006300D800030004>] TJ ET EMC /P <> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 84 11.65499973 Tm [(\(CMC,4%\))] TJ ET EMC /P <> BDC BT /FAAAAI 10.5 Tf 1 0 0 -1 126 11.38799953 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0.5 w 0 0.5 m 0 32.20000076 l S Q q 0.5 w 365 0.5 m 365 32.20000076 l S Q q 0.5 w -0.25 0.25 m 365.25 0.25 l S Q q 0.5 w 0.25 16.35000038 m 364.75 16.35000038 l S Q q 0.5 w -0.25 32.45000076 m 365.25 32.45000076 l S Q Q Q 1 0 0 -1 0 841.90002441 cm endstream endobj 6 0 obj <>stream ÿ??ºÝºÝÈÈÔÚÏßÊÓÆµÃâ·Ñ <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| <Ö©Öë´Ê>| 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