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11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<001A>-4<001B>-4<001C>-4<001D>-4<001E>-4<001F>-3<0020>-4<0021>-4<0022>-4<0023>-4<0024>-4<0025>-4<0026>-4<0027>-4<0024>-4<0028>-3<0029>-4<002A>-4<002B>-4<002C>] TJ ET EMC /P <</MCID 7 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 210.83999634 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 62.40000153 cm /P <</Lang (zh-CN) /MCID 8 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<001B>-4<001C>-4<002D>-4<002E>-4<002F>] TJ ET EMC /P <</MCID 9 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 78 cm /P <</MCID 10 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(Tris)] TJ ET EMC /P <</MCID 11 >> BDC BT 1 0 0 -1 21 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 12 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 26.34600067 11.65499973 Tm [<0006>-9<0007>-9<0033>-9<0034>-10<0008>-9<002D>-9<0035>] TJ ET EMC /P <</MCID 13 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 100.51799774 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 14 >> BDC BT 1 0 0 -1 105.86399841 11.65499973 Tm [(TBS,)] TJ ET EMC /P <</MCID 15 >> BDC BT 1 0 0 -1 126.86399841 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 16 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 132.21000671 11.65499973 Tm [<0036>-9<0028>-9<0037>-9<0038>] TJ ET EMC /P <</MCID 17 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 174.59399414 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 18 >> BDC BT 1 0 0 -1 179.94000244 11.65499973 Tm [(pH)] TJ ET EMC /P <</MCID 19 >> BDC BT 1 0 0 -1 190.44000244 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 20 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 195.78599548 11.65499973 Tm [<0006>-9<0007>-9<0008>-9<0025>] TJ ET EMC /P <</Lang (zh-CN) /MCID 21 >> BDC BT 1 0 0 -1 238.16999817 11.65499973 Tm [<0037>-9<0024>-9<0028>-9<0039>-10<003A>-9<003B>-9<003C>-9<003D>-9<003E>-9<003F>-9<0040>-10<0041>-9<0042>] TJ ET EMC /P <</MCID 22 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 378.54299927 11.65499973 Tm [(Western)] TJ ET EMC /P <</MCID 23 >> BDC BT 1 0 0 -1 415.29299927 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 24 >> BDC BT 1 0 0 -1 0 27.25500107 Tm [(Blot)] TJ ET EMC /P <</MCID 25 >> BDC BT 1 0 0 -1 21 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 26 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 26.25 27.25500107 Tm [<0047003F00480049004A004B004C004D0025004E00370024004F0050005100520053005400550056>] TJ ET EMC /P <</MCID 27 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 236.25 27.25500107 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 109.19999695 cm /P <</MCID 28 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(TBS)] TJ ET EMC /P <</MCID 29 >> BDC BT 1 0 0 -1 15.75 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 30 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 18.375 11.65499973 Tm [<000600070008>] TJ ET EMC /P <</MCID 31 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 49.875 11.65499973 Tm [(\(0.02mol/L,pH8.2\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 32 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 139.125 11.65499973 Tm [<005700580059>] TJ ET EMC /P <</MCID 33 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 170.625 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 34 >> BDC BT 1 0 0 -1 173.25 11.65499973 Tm [(Tris)] TJ ET EMC /P <</Lang (zh-CN) /MCID 35 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 194.25 11.65499973 Tm [<005A>] TJ ET EMC /P <</Lang (zh-CN) /MCID 36 >> BDC BT 1 0 0 -1 203.57499695 11.65499973 Tm [<005B005C005D>] TJ ET EMC /P <</Lang (zh-CN) /MCID 37 >> BDC BT 1 0 0 -1 235.07499695 11.65499973 Tm [<005A>] TJ ET EMC /P <</MCID 38 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 244.3999939 11.65499973 Tm [(BSA)] TJ ET EMC /P <</Lang (zh-CN) /MCID 39 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 260.1499939 11.65499973 Tm [<005A>] TJ ET EMC /P <</Lang (zh-CN) /MCID 40 >> BDC BT 1 0 0 -1 269.4750061 11.65499973 Tm [<005E005F0055004D00400060>] TJ ET EMC /P <</Lang (zh-CN) /MCID 41 >> BDC BT 1 0 0 -1 332.4750061 11.65499973 Tm [<0025>] TJ ET EMC /P <</Lang (zh-CN) /MCID 42 >> BDC BT 1 0 0 -1 341.79998779 11.65499973 Tm [<006100240028003B003C0062003D>] TJ ET EMC /P <</Lang (zh-CN) /MCID 43 >> BDC BT 1 0 0 -1 0 27.25500107 Tm [<003E00470039003A00400041>] TJ ET EMC /P <</Lang (zh-CN) /MCID 44 >> BDC BT 1 0 0 -1 63 27.25500107 Tm [<0025>] TJ ET EMC /P <</Lang (zh-CN) /MCID 45 >> BDC BT 1 0 0 -1 71.93399811 27.25500107 Tm [<0063006100640065006600670008003F0068006900540055>] TJ ET EMC /P <</Lang (zh-CN) /MCID 46 >> BDC BT 1 0 0 -1 197.93400574 27.25500107 Tm [<0056>] TJ ET EMC /P <</Lang (zh-CN) /MCID 47 >> BDC BT 1 0 0 -1 206.86799622 27.25500107 Tm [<006A00540055001D00240028006B0021006C006D>] TJ ET EMC /P <</Lang (zh-CN) /MCID 48 >> BDC BT 1 0 0 -1 311.86801147 27.25500107 Tm [<0025>] TJ ET EMC /P <</Lang (zh-CN) /MCID 49 >> BDC BT 1 0 0 -1 320.80200195 27.25500107 Tm [<0026006E002400280029002A002B002C0042>] TJ ET EMC /P <</Lang (zh-CN) /MCID 50 >> BDC BT 1 0 0 -1 0 42.85499954 Tm [<006F0070002400710056>] TJ ET EMC /P <</MCID 51 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 52.5 42.85499954 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 156 cm /P <</MCID 52 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 171.6000061 cm /P <</Lang (zh-CN) /MCID 53 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<001B>-4<001C>-4<0040>-4<0060>-4<002F>] TJ ET EMC /P <</MCID 54 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 392.70001221 187.19999695 cm /P <</MCID 55 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 392.70001221 202.80000305 cm /P <</MCID 56 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 392.70001221 218.3999939 cm /P <</MCID 57 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</MCID 58 >> BDC BT 1 0 0 -1 21 11.65499973 Tm [( )] TJ ET EMC Q q 1 0 0 1 392.70001221 234 cm /P <</MCID 59 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</MCID 60 >> BDC BT 1 0 0 -1 0 27.25500107 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 61 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 -392.70001221 42.85499954 Tm 2 Tr /GS2 gs 0 g 0.30000001 w 0 G [<0072>-4<0064>-4<0073>-4<0074>] TJ ET EMC /P <</MCID 62 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 -350.53201294 42.85499954 Tm [(\()] TJ ET EMC /P <</Lang (zh-CN) /MCID 63 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 -345.24002075 42.85499954 Tm [<001D>-4<001E>-4<0075>-4<0076>] TJ ET EMC /P <</MCID 64 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 -303.07202148 42.85499954 Tm [(\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 65 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 -297.7800293 42.85499954 Tm [<002F>] TJ ET EMC /P <</MCID 66 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 -287.23800659 42.85499954 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 280.79998779 cm /P <</MCID 67 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 68 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<005A00780079007A007B007C0079007D007E0056>] TJ ET EMC /P <</MCID 69 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 110.25 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 296.3999939 cm /P <</MCID 70 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(2)] TJ ET EMC /P <</Lang (zh-CN) /MCID 71 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<005A>] TJ ET EMC /P <</MCID 72 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 15.75 11.65499973 Tm [(0.1%)] TJ ET EMC /P <</Lang (zh-CN) /MCID 73 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 36.75 11.65499973 Tm [<00800048004900810082005C>] TJ ET EMC /P <</MCID 74 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 99.75 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 75 >> BDC BT 1 0 0 -1 105 11.65499973 Tm [(10min)] TJ ET EMC /P <</MCID 76 >> BDC BT 1 0 0 -1 131.25 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 77 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 136.5 11.65499973 Tm [<0042>] TJ ET EMC /P <</MCID 78 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 147 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 79 >> BDC BT 1 0 0 -1 152.25 11.65499973 Tm [(3M)] TJ ET EMC /P <</MCID 80 >> BDC BT 1 0 0 -1 162.75 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 81 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 168 11.65499973 Tm [<008500860082005C>] TJ ET EMC /P <</MCID 82 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 210 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 83 >> BDC BT 1 0 0 -1 215.25 11.65499973 Tm [(30min)] TJ ET EMC /P <</Lang (zh-CN) /MCID 84 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 241.5 11.65499973 Tm [<0056>] TJ ET EMC /P <</MCID 85 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 252 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 312 cm /P <</MCID 86 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(3)] TJ ET EMC /P <</Lang (zh-CN) /MCID 87 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<005A00870088007E0007003A>] TJ ET EMC /P <</MCID 88 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 68.25 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 89 >> BDC BT 1 0 0 -1 73.5 11.65499973 Tm [(2)] TJ ET EMC /P <</MCID 90 >> BDC BT 1 0 0 -1 78.75 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 91 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 84 11.65499973 Tm [<00890025008A0089>] TJ ET EMC /P <</MCID 92 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 126 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 93 >> BDC BT 1 0 0 -1 131.25 11.65499973 Tm [(5)] TJ ET EMC /P <</Lang (zh-CN) /MCID 94 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 136.5 11.65499973 Tm [<008C>] TJ ET EMC /P <</MCID 95 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 147 11.65499973 Tm [(10min)] TJ ET EMC /P <</Lang (zh-CN) /MCID 96 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 173.25 11.65499973 Tm [<0056>] TJ ET EMC /P <</MCID 97 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 183.75 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 327.6000061 cm /P <</MCID 98 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(4)] TJ ET EMC /P <</Lang (zh-CN) /MCID 99 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<005A>] TJ ET EMC /P <</MCID 100 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 15.75 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 101 >> BDC BT 1 0 0 -1 21 11.65499973 Tm [(TBS)] TJ ET EMC /P <</MCID 102 >> BDC BT 1 0 0 -1 36.75 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 103 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 42 11.65499973 Tm [<000600070008>] TJ ET EMC /P <</MCID 104 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 73.5 11.65499973 Tm [(\(0.02mol/L,pH8.2\))] TJ ET EMC /P <</Lang (zh-CN) /MCID 105 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 162.75 11.65499973 Tm [<008E008F003A0090>] TJ ET EMC /P <</MCID 106 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 204.75 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 107 >> BDC BT 1 0 0 -1 210 11.65499973 Tm [(2)] TJ ET EMC /P <</MCID 108 >> BDC BT 1 0 0 -1 215.25 11.65499973 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 109 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 220.5 11.65499973 Tm [<00890025008A0089>] TJ ET EMC /P <</MCID 110 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 262.5 11.65499973 Tm [( )] TJ ET EMC /P <</MCID 111 >> BDC BT 1 0 0 -1 267.75 11.65499973 Tm [(10min)] TJ ET EMC /P <</Lang (zh-CN) /MCID 112 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 294 11.65499973 Tm [<0056>] TJ ET EMC /P <</MCID 113 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 304.5 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 343.20001221 cm /P <</MCID 114 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(5)] TJ ET EMC /P <</Lang (zh-CN) /MCID 115 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<005A0091009200660067009300940095006F0070009600970056>] TJ ET EMC /P <</MCID 116 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 141.75 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 358.79998779 cm /P <</MCID 117 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 1 Tr 0.30000001 w /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 374.3999939 cm /P <</Lang (zh-CN) /MCID 118 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<0098>-4<0099>-4<009A>-4<009B>-4<002F>] TJ ET EMC /P <</MCID 119 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 390 cm /P <</MCID 120 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 121 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<005A009C009D008A0089003F00230024009E009F00A00025006100A1006E00A2004F00A300A400A5002300240056>] TJ ET EMC /P <</MCID 122 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 246.75 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 405.6000061 cm /P <</MCID 123 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(2)] TJ ET EMC /P <</Lang (zh-CN) /MCID 124 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 5.25 11.65499973 Tm [<005A006500A600A7003F00A800A900AA00AB00AC002500AD00AE0096009700AF00B000B100B2008900B300B400B5007200640056>] TJ ET EMC /P <</MCID 125 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 278.25 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 421.20001221 cm /P <</MCID 126 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 1 Tr 0.30000001 w /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 436.79998779 cm /P <</Lang (zh-CN) /MCID 127 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 2 Tr 0 g 0.30000001 w 0 G [<00B6>-4<00B7>-4<001B>-4<001C>-4<002F>] TJ ET EMC /P <</MCID 128 >> BDC BT /FAAABH 10.5 Tf 1 0 0 -1 52.70999908 11.65499973 Tm 1 Tr /GS3 gs 0 G [( )] TJ ET EMC Q q 1 0 0 1 0 452.3999939 cm /P <</MCID 129 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 1.04999995 468 cm q 0.25 0.5 m 380.75 0.5 l 380.75 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 130 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(Sorensen)] TJ ET EMC /P <</Lang (zh-CN) /MCID 131 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 44.625 11.65499973 Tm [<00B800B9000600070008>] TJ ET EMC /P <</MCID 132 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 97.125 11.65499973 Tm [(\(10)] TJ ET EMC /P <</Lang (zh-CN) /MCID 133 >> BDC BT 1 0 0 -1 112.875 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 134 >> BDC BT 1 0 0 -1 123.375 11.65499973 Tm [(,pH6.2\))] TJ ET EMC /P <</MCID 135 >> BDC BT 1 0 0 -1 160.125 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 16.10000038 cm q 0.25 0.5 m 380.75 0.5 l 380.75 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 136 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(Sorensen)] TJ ET EMC /P <</Lang (zh-CN) /MCID 137 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 44.625 11.65499973 Tm [<00B800B9000600070008>] TJ ET EMC /P <</MCID 138 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 97.125 11.65499973 Tm [(\(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 139 >> BDC BT 1 0 0 -1 107.625 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 140 >> BDC BT 1 0 0 -1 118.125 11.65499973 Tm [(,pH6.2\))] TJ ET EMC /P <</MCID 141 >> BDC BT 1 0 0 -1 154.875 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 32.20000076 cm q 0.25 0.5 m 380.75 0.5 l 380.75 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 142 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(TBS)] TJ ET EMC /P <</Lang (zh-CN) /MCID 143 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 18.375 11.65499973 Tm [<000600070008>] TJ ET EMC /P <</MCID 144 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 49.875 11.65499973 Tm [(\(0.02mol_L,pH8.2\))] TJ ET EMC /P <</MCID 145 >> BDC BT 1 0 0 -1 139.125 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 48.29999924 cm q 0.25 0.5 m 380.75 0.5 l 380.75 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 146 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(TBS)] TJ ET EMC /P <</Lang (zh-CN) /MCID 147 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 18.375 11.65499973 Tm [<000600070008>] TJ ET EMC /P <</MCID 148 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 49.875 11.65499973 Tm [(\(0.05mol_L,pH7.4\))] TJ ET EMC /P <</MCID 149 >> BDC BT 1 0 0 -1 139.125 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 64.40000153 cm q 0.25 0.5 m 380.75 0.5 l 380.75 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 150 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(Tris-EDTA)] TJ ET EMC /P <</Lang (zh-CN) /MCID 151 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 49.875 11.65499973 Tm [<00C100C200C300C40008>] TJ ET EMC /P <</MCID 152 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 102.375 11.65499973 Tm [(\(10)] TJ ET EMC /P <</Lang (zh-CN) /MCID 153 >> BDC BT 1 0 0 -1 118.125 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 154 >> BDC BT 1 0 0 -1 128.625 11.65499973 Tm [(,pH8.0\))] TJ ET EMC /P <</MCID 155 >> BDC BT 1 0 0 -1 165.375 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 1 0 0 1 0 80.5 cm q 0.25 0.5 m 380.75 0.5 l 380.75 16.10000038 l 0.25 16.10000038 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 156 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm 0 g [(Tris-EDTA)] TJ ET EMC /P <</Lang (zh-CN) /MCID 157 >> BDC BT /FAAACG 10.5 Tf 1 0 0 -1 49.875 11.65499973 Tm [<00C100C200C300C40008>] TJ ET EMC /P <</MCID 158 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 102.375 11.65499973 Tm [(\(10)] TJ ET EMC /P <</Lang (zh-CN) /MCID 159 >> BDC BT 1 0 0 -1 118.125 11.65499973 Tm [(?] TJ ET EMC /P <</MCID 160 >> BDC BT 1 0 0 -1 128.625 11.65499973 Tm [(,pH8.5\))] TJ ET EMC /P <</MCID 161 >> BDC BT 1 0 0 -1 165.375 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0 G 0.5 w 0 0.5 m 0 96.59999847 l S Q q 0 G 0.5 w 381 0.5 m 381 96.59999847 l S Q q 0 G 0.5 w -0.25 0.25 m 381.25 0.25 l S Q q 0 G 0.5 w 0.25 16.35000038 m 380.75 16.35000038 l S Q q 0 G 0.5 w 0.25 32.45000076 m 380.75 32.45000076 l S Q q 0 G 0.5 w 0.25 48.55000305 m 380.75 48.55000305 l S Q q 0 G 0.5 w 0.25 64.65000153 m 380.75 64.65000153 l S Q q 0 G 0.5 w 0.25 80.75 m 380.75 80.75 l S Q q 0 G 0.5 w -0.25 96.84999847 m 381.25 96.84999847 l S Q Q q 1 0 0 1 0 565.09997559 cm /P <</MCID 162 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 580.70001221 cm /P <</MCID 163 >> BDC BT /FAAACE 10.5 Tf 1 0 0 -1 0 11.65499973 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 596.29998779 cm Q Q q 1 0 0 1 90.55000305 327.24398804 cm q 0.25 0.5 m 133.05000305 0.5 l 133.05000305 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 164 >> BDC BT /FAAACG 9 Tf 1 0 0 -1 43.25 15.00500011 Tm 0 g [<001B001C00C50035>] TJ ET EMC /P <</MCID 165 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 79.25 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 133.30000305 0 cm 0.25 0.5 m 66.40000153 0.5 l 66.40000153 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 166 >> BDC BT /FAAACG 9 Tf 1 0 0 -1 18.92499924 15.00500011 Tm 0 g [<003800C6>] TJ ET EMC /P <</MCID 167 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 36.92499924 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 199.94999695 0 cm 0.25 0.5 m 60.34999847 0.5 l 60.34999847 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 168 >> BDC BT /FAAACG 9 Tf 1 0 0 -1 11.39999962 15.00500011 Tm 0 g [<001700180019>] TJ ET EMC /P <</MCID 169 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 38.40000153 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 260.54998779 0 cm 0.25 0.5 m 67.09999847 0.5 l 67.09999847 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 170 >> BDC BT /FAAACG 9 Tf 1 0 0 -1 14.77499962 15.00500011 Tm 0 g [<00C700C800C9>] TJ ET EMC /P <</MCID 171 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 41.77500153 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 327.8999939 0 cm 0.25 0.5 m 54.75 0.5 l 54.75 23.89999962 l 0.25 23.89999962 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</Lang (zh-CN) /MCID 172 >> BDC BT /FAAACG 9 Tf 1 0 0 -1 4.0999999 15.00500011 Tm 0 g [<00CA00CB00CC00CD>] TJ ET EMC /P <</MCID 173 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 40.09999847 15.00500011 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 0 23.89999962 cm q 0.25 0.5 m 133.05000305 0.5 l 133.05000305 31.20000076 l 0.25 31.20000076 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 174 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 1.625 18.65500069 Tm 0 g [(TBS)] TJ ET EMC /P <</Lang (zh-CN) /MCID 175 >> BDC BT /FAAACG 9 Tf 1 0 0 -1 17.375 18.65500069 Tm [<000600070008>] TJ ET EMC /P <</MCID 176 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 44.375 18.65500069 Tm [(\(0.02mol/L,pH8.2\))] TJ ET EMC /P <</MCID 177 >> BDC BT 1 0 0 -1 120.875 18.65500069 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 133.30000305 0 cm 0.25 0.5 m 66.40000153 0.5 l 66.40000153 31.20000076 l 0.25 31.20000076 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 178 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 16.67499924 18.65500069 Tm 0 g [(500ml)] TJ ET EMC /P <</MCID 179 >> BDC BT 1 0 0 -1 39.17499924 18.65500069 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 199.94999695 0 cm 0.25 0.5 m 60.34999847 0.5 l 60.34999847 31.20000076 l 0.25 31.20000076 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 180 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 17.02499962 18.65500069 Tm 0 g [(1)] TJ ET EMC /P <</Lang (zh-CN) /MCID 181 >> BDC BT /FAAACG 9 Tf 1 0 0 -1 23.77499962 18.65500069 Tm [<00CE>] TJ ET EMC /P <</MCID 182 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 32.77500153 18.65500069 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 260.54998779 0 cm 0.25 0.5 m 67.09999847 0.5 l 67.09999847 31.20000076 l 0.25 31.20000076 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 183 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 13.64999962 18.65500069 Tm 0 g [(12)] TJ ET EMC /P <</Lang (zh-CN) /MCID 184 >> BDC BT /FAAACG 9 Tf 1 0 0 -1 24.89999962 18.65500069 Tm [<00CF00D0>] TJ ET EMC /P <</MCID 185 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 42.90000153 18.65500069 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 1 0 0 1 327.8999939 0 cm 0.25 0.5 m 54.75 0.5 l 54.75 31.20000076 l 0.25 31.20000076 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 186 >> BDC BT /FAAACE 9 Tf 1 0 0 -1 17.60000038 18.65500069 Tm 0 g [(RT)] TJ ET EMC /P <</MCID 187 >> BDC BT 1 0 0 -1 26.60000038 18.65500069 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q Q q 0 G 0.5 w 0 0.5 m 0 55.09999847 l S Q q 0 G 0.5 w 133.30000305 0.5 m 133.30000305 23.89999962 l S Q q 0 G 0.5 w 133.30000305 24.39999962 m 133.30000305 55.09999847 l S Q q 0 G 0.5 w 199.95001221 0.5 m 199.95001221 23.89999962 l S Q q 0 G 0.5 w 199.95001221 24.39999962 m 199.95001221 55.09999847 l S Q q 0 G 0.5 w 260.54998779 0.5 m 260.54998779 23.89999962 l S Q q 0 G 0.5 w 260.54998779 24.39999962 m 260.54998779 55.09999847 l S Q q 0 G 0.5 w 327.8999939 0.5 m 327.8999939 23.89999962 l S Q q 0 G 0.5 w 327.8999939 24.39999962 m 327.8999939 55.09999847 l S Q q 0 G 0.5 w 382.8999939 0.5 m 382.8999939 55.09999847 l S Q q 0 G 0.5 w -0.25 0.25 m 383.1499939 0.25 l S Q q 0 G 0.5 w 0.25 24.14999962 m 382.6499939 24.14999962 l S Q q 0 G 0.5 w -0.25 55.34999847 m 383.1499939 55.34999847 l S Q Q 1 0 0 -1 0 841.90002441 cm 
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