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ET EMC /P <</MCID 25 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 52.5 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 26 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 63 12.82499981 Tm /GS2 gs 0 g [<00120013>] TJ ET EMC /P <</MCID 27 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 84 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</MCID 28 >> BDC BT 1 0 0 -1 105 12.82499981 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 180.95599365 cm /P <</Lang (zh-CN) /MCID 29 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 12.82499981 Tm /GS2 gs 0 g [<0014>] TJ ET EMC /P <</MCID 30 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 10.5 12.82499981 Tm [(  )] TJ ET EMC /P <</Lang (zh-CN) /MCID 31 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 21 12.82499981 Tm [<0015000C>] TJ ET EMC /P <</MCID 32 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 42 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 33 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 63 12.82499981 Tm /GS2 gs 0 g [<001600170018>] TJ ET EMC /P <</MCID 34 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 94.5 12.82499981 Tm [(-20)] TJ ET EMC /P <</Lang (zh-CN) /MCID 35 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 108.35800171 12.82499981 Tm [<0019001A>] TJ ET EMC /P <</MCID 36 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 129.35800171 12.82499981 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 37 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 134.60800171 12.82499981 Tm [<001B001C>] TJ ET EMC /P <</MCID 38 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 155.60800171 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q /GS2 gs 1 g -1.45000005 214.49499512 m 416.75 214.49499512 l 416.75 245.69499207 l -1.45000005 245.69499207 l h f* Q q 1 0 0 1 0 214.49499512 cm /P <</MCID 39 >> BDC q /GS2 gs 1 g 0 6.59399986 m 21 6.59399986 l 21 24.60499954 l 0 24.60499954 l h f* Q EMC /P <</Lang (zh-CN) /MCID 40 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 20.17499924 Tm /GS2 gs 0.13333334 g [<00030004>] TJ ET EMC /P <</MCID 41 >> BDC BT /FAAACJ 10.5 Tf 1 0 0 -1 21 20.17499924 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 245.69500732 cm /P <</MCID 42 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 -5.4000001 273.63400269 cm q -0.75 -0.5 m 426.8500061 -0.5 l 426.8500061 29.43899918 l -0.75 29.43899918 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 43 >> BDC BT /FAAAAH 9 Tf 1 0 0 -1 0 12.04500008 Tm /GS2 gs 0 g [(SKOV3)] TJ ET EMC /P <</MCID 44 >> BDC BT 1 0 0 -1 24.43799973 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 45 >> BDC BT 1 0 0 -1 26.47299957 12.04500008 Tm [(ce)1(l)-1(l)] TJ ET EMC /P <</MCID 46 >> BDC BT 1 0 0 -1 38.88800049 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 47 >> BDC BT 1 0 0 -1 40.92300034 12.04500008 Tm [(lysates)] TJ ET EMC /P <</MCID 48 >> BDC BT 1 0 0 -1 65.90599823 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 49 >> BDC BT 1 0 0 -1 67.94100189 12.04500008 Tm [(from)] TJ ET EMC /P <</MCID 50 >> BDC BT 1 0 0 -1 85.76100159 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 51 >> BDC BT 1 0 0 -1 87.79599762 12.04500008 Tm [(hu)-1(man)] TJ ET EMC /P <</MCID 52 >> BDC BT 1 0 0 -1 113.48200226 12.04500008 Tm [( )] TJ ET EMC /P 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251.30200195 12.04500008 Tm [(the)] TJ ET EMC /P <</MCID 66 >> BDC BT 1 0 0 -1 263.52301025 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 67 >> BDC BT 1 0 0 -1 265.55801392 12.04500008 Tm [(me)1(d)-1(ia)] TJ ET EMC /P <</MCID 68 >> BDC BT 1 0 0 -1 288.32998657 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 69 >> BDC BT 1 0 0 -1 290.36499023 12.04500008 Tm [(con)-1(taini)-1(ng)] TJ ET EMC /P <</MCID 70 >> BDC BT 1 0 0 -1 328.79501343 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 71 >> BDC BT 1 0 0 -1 330.82998657 12.04500008 Tm [(10%)] TJ ET EMC /P <</MCID 72 >> BDC BT 1 0 0 -1 346.38699341 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 73 >> BDC BT 1 0 0 -1 348.42199707 12.04500008 Tm [(FBS)-1(.)] TJ ET EMC /P <</MCID 74 >> BDC BT /FAAACJ 9 Tf 1 0 0 -1 363.85998535 12.04500008 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 0.5 w 0 0.5 m 0 28.43899918 l S Q q 0.5 w 426.1000061 0.5 m 426.1000061 28.43899918 l S Q q 0.5 w -0.25 0.25 m 426.3500061 0.25 l S Q q 0.5 w -0.25 28.68899918 m 426.3500061 28.68899918 l S Q Q 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11.72200012 Tm [(lyse)-1(d)] TJ ET EMC /P <</MCID 88 >> BDC BT 1 0 0 -1 72.02600098 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 89 >> BDC BT 1 0 0 -1 76.52600098 11.72200012 Tm [(in)] TJ ET EMC /P <</MCID 90 >> BDC BT 1 0 0 -1 84.46700287 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 91 >> BDC BT 1 0 0 -1 88.96700287 11.72200012 Tm [(lysis)] TJ ET EMC /P <</MCID 92 >> BDC BT 1 0 0 -1 106.85299683 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 93 >> BDC BT 1 0 0 -1 111.35299683 11.72200012 Tm [(buff)1(e)-1(r)] TJ ET EMC /P <</MCID 94 >> BDC BT 1 0 0 -1 137.42999268 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 95 >> BDC BT 1 0 0 -1 141.92999268 11.72200012 Tm [(\(50)-1(mM)] TJ ET EMC /P <</MCID 96 >> BDC BT 1 0 0 -1 172.71800232 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 97 >> BDC BT 1 0 0 -1 177.21800232 11.72200012 Tm [(Tris\(PH7)-1(.)1(4)-1(\),)] TJ ET EMC /P <</MCID 98 >> BDC BT 1 0 0 -1 225.74200439 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 99 >> BDC BT 1 0 0 -1 230.24200439 11.72200012 Tm 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14.86699963 27.3220005 Tm [(10)-1(0)] TJ ET EMC /P <</MCID 113 >> BDC BT 1 0 0 -1 30.70000076 27.3220005 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 114 >> BDC BT /FAAADE 9 Tf 1 0 0 -1 35.20000076 27.3220005 Tm [<0024>] TJ ET EMC /P <</MCID 115 >> BDC BT /FAAADC 9 Tf 1 0 0 -1 40.85100174 27.3220005 Tm [(g)] TJ ET EMC /P <</MCID 116 >> BDC BT 1 0 0 -1 46.60800171 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 117 >> BDC BT 1 0 0 -1 51.10800171 27.3220005 Tm [(of)] TJ ET EMC /P <</MCID 118 >> BDC BT 1 0 0 -1 59.95000076 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 119 >> BDC BT 1 0 0 -1 64.44999695 27.3220005 Tm [(ce)-1(llular)] TJ ET EMC /P <</MCID 120 >> BDC BT 1 0 0 -1 95.21199799 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 121 >> BDC BT 1 0 0 -1 99.71199799 27.3220005 Tm [(pro)1(tein)] TJ ET EMC /P <</MCID 122 >> BDC BT 1 0 0 -1 131.01899719 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 123 >> BDC BT 1 0 0 -1 135.51899719 27.3220005 Tm [(wa)1(s)] TJ ET EMC /P <</MCID 124 >> BDC BT 1 0 0 -1 151.76600647 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 125 >> BDC BT 1 0 0 -1 156.26600647 27.3220005 Tm [(mixed)] TJ ET EMC /P <</MCID 126 >> BDC BT 1 0 0 -1 182.51899719 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 127 >> BDC BT 1 0 0 -1 187.01899719 27.3220005 Tm [(wit)1(h)] TJ ET EMC /P <</MCID 128 >> BDC BT 1 0 0 -1 205.41499329 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 129 >> BDC BT 1 0 0 -1 209.91499329 27.3220005 Tm [(4)] TJ ET EMC /P <</MCID 130 >> BDC BT 1 0 0 -1 215.19299316 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 131 >> BDC BT 1 0 0 -1 219.69299316 27.3220005 Tm [(times)] TJ ET EMC /P <</MCID 132 >> BDC BT 1 0 0 -1 243.14700317 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 133 >> BDC BT 1 0 0 -1 247.64700317 27.3220005 Tm [(volume)] TJ ET EMC /P <</MCID 134 >> BDC BT 1 0 0 -1 279.57299805 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 135 >> BDC BT 1 0 0 -1 284.07299805 27.3220005 Tm [(of)] TJ ET EMC /P <</MCID 136 >> BDC BT 1 0 0 -1 292.91500854 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 137 >> BDC BT 1 0 0 -1 297.41500854 27.3220005 Tm [(5X)] TJ ET EMC /P <</MCID 138 >> BDC BT 1 0 0 -1 308.49798584 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 139 >> BDC BT 1 0 0 -1 312.99798584 27.3220005 Tm [(SDS-P)1(AGE)] TJ ET EMC /P <</MCID 140 >> BDC BT 1 0 0 -1 357.61599731 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 141 >> BDC BT 1 0 0 -1 362.11599731 27.3220005 Tm [(loa)] TJ ET EMC /P <</MCID 142 >> BDC BT 1 0 0 -1 0 42.92200089 Tm [(ding)] TJ ET EMC /P <</MCID 143 >> BDC BT 1 0 0 -1 19.45499992 42.92200089 Tm [( )] TJ ET EMC /P <</MCID 144 >> BDC BT 1 0 0 -1 23.95499992 42.92200089 Tm [(buff)1(e)-1(r)1(.)] TJ ET EMC /P <</MCID 145 >> BDC BT 1 0 0 -1 52.19900131 42.92200089 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 0 0.75 m 0 47.54999924 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 36.70000076 0.75 m 36.70000076 47.54999924 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 422.80001831 0.75 m 422.80001831 47.54999924 l S Q q /GS1 gs 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