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12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 113.87799835 cm /P <</Lang (zh-CN) /MCID 16 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 12.82499981 Tm /GS2 gs 0 g [<000E>] TJ ET EMC /P <</MCID 17 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 10.5 12.82499981 Tm [(    )] TJ ET EMC /P <</Lang (zh-CN) /MCID 18 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 31.5 12.82499981 Tm [<000A000C>] TJ ET EMC /P <</MCID 19 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 52.5 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</MCID 20 >> BDC BT 1 0 0 -1 63 12.82499981 Tm /GS2 gs 0 g [(Jurk)1(at)] TJ ET EMC /P <</Lang (zh-CN) /MCID 21 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 91.47000122 12.82499981 Tm [<00030004000500060007>] TJ ET EMC /P <</MCID 22 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 143.97000122 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 147.41700745 cm /P <</Lang (zh-CN) /MCID 23 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 12.82499981 Tm /GS2 gs 0 g [<000F00100011000E000C>] TJ ET EMC /P <</MCID 24 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 52.5 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 25 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 63 12.82499981 Tm /GS2 gs 0 g [<00120013>] TJ ET EMC /P <</MCID 26 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 84 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</MCID 27 >> BDC BT 1 0 0 -1 105 12.82499981 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 180.95599365 cm /P <</Lang (zh-CN) /MCID 28 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 12.82499981 Tm /GS2 gs 0 g [<0014>] TJ ET EMC /P <</MCID 29 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 10.5 12.82499981 Tm [(    )] TJ ET EMC /P <</Lang (zh-CN) /MCID 30 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 31.5 12.82499981 Tm [<0015000C>] TJ ET EMC /P <</MCID 31 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 52.5 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 32 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 63 12.82499981 Tm /GS2 gs 0 g [<001600170018>] TJ ET EMC /P <</MCID 33 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 94.5 12.82499981 Tm [(-20)] TJ ET EMC /P <</Lang (zh-CN) /MCID 34 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 108.35800171 12.82499981 Tm [<0019001A>] TJ ET EMC /P <</MCID 35 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 129.35800171 12.82499981 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 36 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 134.60800171 12.82499981 Tm [<001B001C>] TJ ET EMC /P <</MCID 37 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 155.60800171 12.82499981 Tm [( )] TJ ET EMC /P <</MCID 38 >> BDC BT 1 0 0 -1 160.85800171 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q /GS2 gs 1 g -1.45000005 214.49499512 m 416.75 214.49499512 l 416.75 245.69499207 l -1.45000005 245.69499207 l h f* Q q 1 0 0 1 0 214.49499512 cm /P <</MCID 39 >> BDC q /GS2 gs 1 g 0 6.59399986 m 21 6.59399986 l 21 24.60499954 l 0 24.60499954 l h f* Q EMC /P <</Lang (zh-CN) /MCID 40 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 20.17499924 Tm /GS2 gs 0.13333334 g [<00030004>] TJ ET EMC /P <</MCID 41 >> BDC BT /FAAACJ 10.5 Tf 1 0 0 -1 21 20.17499924 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 245.69500732 cm /P <</MCID 42 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 -5.4000001 273.63400269 cm q -0.75 -0.5 m 426.8500061 -0.5 l 426.8500061 29.43899918 l -0.75 29.43899918 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 43 >> BDC BT /FAAAAH 9 Tf 1 0 0 -1 0 12.04500008 Tm /GS2 gs 0 g [(Jurk)1(at)] TJ ET EMC /P <</MCID 44 >> BDC BT 1 0 0 -1 22.15299988 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 45 >> BDC BT 1 0 0 -1 24.18799973 12.04500008 Tm [(ce)1(l)-1(l)] TJ ET EMC /P <</MCID 46 >> BDC BT 1 0 0 -1 36.60300064 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 47 >> BDC BT 1 0 0 -1 38.63800049 12.04500008 Tm [(lysates)] TJ ET EMC /P <</MCID 48 >> BDC BT 1 0 0 -1 63.62099838 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 49 >> BDC BT 1 0 0 -1 65.65599823 12.04500008 Tm [(from)] TJ ET EMC /P <</MCID 50 >> BDC BT 1 0 0 -1 83.47599792 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 51 >> BDC BT 1 0 0 -1 85.51100159 12.04500008 Tm [(hu)-1(man)] TJ ET EMC /P <</MCID 52 >> BDC BT 1 0 0 -1 111.1969986 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 53 >> BDC BT 1 0 0 -1 113.23200226 12.04500008 Tm [(periph)-1(e)1(ral)] TJ ET EMC /P <</MCID 54 >> BDC BT 1 0 0 -1 151.09100342 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 55 >> BDC BT 1 0 0 -1 153.12600708 12.04500008 Tm [(bl)-1(oo)-1(d)] TJ ET EMC /P <</MCID 56 >> BDC BT 1 0 0 -1 174.14100647 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 57 >> BDC BT 1 0 0 -1 176.17599487 12.04500008 Tm [(leukem)1(i)-1(a)] TJ ET EMC /P <</MCID 58 >> BDC BT 1 0 0 -1 209.58299255 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 59 >> BDC BT 1 0 0 -1 211.61799622 12.04500008 Tm [(T)] TJ ET EMC /P <</MCID 60 >> BDC BT 1 0 0 -1 216.0039978 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 61 >> BDC BT 1 0 0 -1 218.03900146 12.04500008 Tm [(ce)1(l)-1(ls)] TJ ET EMC /P <</MCID 62 >> BDC BT 1 0 0 -1 233.97399902 12.04500008 Tm [( )] TJ ET EMC /P <</MCID 63 >> BDC BT 1 0 0 -1 236.00900269 12.04500008 Tm [(we)1(re)] TJ ET EMC /P <</MCID 64 >> BDC BT 1 0 0 -1 254.53599548 12.04500008 Tm [( )] TJ ET EMC 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395.24200439 12.04500008 Tm [(FBS)-1(.)] TJ ET EMC /P <</MCID 78 >> BDC BT /FAAACJ 10.5 Tf 1 0 0 -1 410.67999268 12.04500008 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 0.5 w 0 0.5 m 0 28.43899918 l S Q q 0.5 w 426.1000061 0.5 m 426.1000061 28.43899918 l S Q q 0.5 w -0.25 0.25 m 426.3500061 0.25 l S Q q 0.5 w -0.25 28.68899918 m 426.3500061 28.68899918 l S Q Q q /GS2 gs 1 g -1.45000005 302.57299805 m 416.75 302.57299805 l 416.75 333.77301025 l -1.45000005 333.77301025 l h f* Q q 1 0 0 1 0 302.57299805 cm /P <</MCID 79 >> BDC BT /FAAACJ 10.5 Tf 1 0 0 -1 0 20.17499924 Tm [( )] TJ ET EMC Q q /GS2 gs 1 g -1.45000005 333.77301025 m 416.75 333.77301025 l 416.75 364.97302246 l -1.45000005 364.97302246 l h f* Q q 1 0 0 1 0 333.77301025 cm /P <</MCID 80 >> BDC q /GS2 gs 1 g 0 6.59399986 m 42 6.59399986 l 42 24.60499954 l 0 24.60499954 l h f* Q EMC /P <</Lang (zh-CN) /MCID 81 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 20.17499924 Tm /GS2 gs 0.13333334 g [<0005000600070008>] TJ ET EMC /P <</MCID 82 >> BDC BT /FAAACJ 10.5 Tf 1 0 0 -1 42 20.17499924 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 -3.75 364.97299194 cm q /GS2 gs 1 g 0.375 0.75 m 63.67499924 0.75 l 63.67499924 95.09999847 l 0.375 95.09999847 l h f* Q q -0.625 -0.25 m 64.67500305 -0.25 l 64.67500305 96.09999847 l -0.625 96.09999847 l h W n q 1 0 0 1 3.75 0.75 cm /P <</MCID 83 >> BDC BT /FAAACJ 9 Tf 1 0 0 -1 0 51.09700012 Tm /GS2 gs 0.13333334 g [(Ba)1(ckgr)1(o)-1(und)] TJ ET EMC /P <</MCID 84 >> BDC BT /FAAADC 9 Tf 1 0 0 -1 54.72100067 51.09700012 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q /GS2 gs 1 g 64.42500305 0.75 m 422.42498779 0.75 l 422.42498779 95.09999847 l 64.42500305 95.09999847 l h f* Q q 1 0 0 1 64.05000305 0 cm -0.625 -0.25 m 359.375 -0.25 l 359.375 96.09999847 l -0.625 96.09999847 l h W n q 1 0 0 1 3.75 0.75 cm /P <</MCID 85 >> BDC BT /FAAADC 9 Tf 1 0 0 -1 0 35.49700165 Tm /GS2 gs 0.13333334 g [(Cell)-1(s)] TJ ET EMC /P <</MCID 86 >> BDC BT 1 0 0 -1 20.08300018 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 87 >> BDC BT 1 0 0 -1 24.58300018 35.49700165 Tm [(were)] TJ ET EMC /P <</MCID 88 >> BDC BT 1 0 0 -1 45.33800125 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 89 >> BDC BT 1 0 0 -1 49.83800125 35.49700165 Tm [(lyse)-1(d)] TJ ET EMC /P <</MCID 90 >> BDC BT 1 0 0 -1 72.02600098 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 91 >> BDC BT 1 0 0 -1 76.52600098 35.49700165 Tm [(in)] TJ ET EMC /P <</MCID 92 >> BDC BT 1 0 0 -1 84.46700287 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 93 >> BDC BT 1 0 0 -1 88.96700287 35.49700165 Tm [(lysis)] TJ ET EMC /P <</MCID 94 >> BDC BT 1 0 0 -1 106.85299683 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 95 >> BDC BT 1 0 0 -1 111.35299683 35.49700165 Tm [(buff)1(e)-1(r)] TJ ET EMC /P <</MCID 96 >> BDC BT 1 0 0 -1 137.42999268 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 97 >> BDC BT 1 0 0 -1 141.92999268 35.49700165 Tm [(\(50)-1(mM)] TJ ET EMC /P <</MCID 98 >> BDC BT 1 0 0 -1 172.71800232 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 99 >> BDC BT 1 0 0 -1 177.21800232 35.49700165 Tm [(Tris\(PH7)-1(.)1(4)-1(\),)] TJ ET EMC /P <</MCID 100 >> BDC BT 1 0 0 -1 225.74200439 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 101 >> BDC BT 1 0 0 -1 230.24200439 35.49700165 Tm [(15)-1(0mM)] TJ ET EMC /P <</MCID 102 >> BDC BT 1 0 0 -1 263.30200195 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 103 >> BDC BT 1 0 0 -1 267.80200195 35.49700165 Tm [(NaCl,)] TJ ET EMC /P <</MCID 104 >> BDC BT 1 0 0 -1 290.67599487 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 105 >> BDC BT 1 0 0 -1 295.17599487 35.49700165 Tm [(1%)] TJ ET EMC /P <</MCID 106 >> BDC BT 1 0 0 -1 308.46099854 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 107 >> BDC BT 1 0 0 -1 312.96099854 35.49700165 Tm [(NP-)1(4)-1(0,)] TJ ET EMC /P <</MCID 108 >> BDC BT 1 0 0 -1 342.3999939 35.49700165 Tm [( )] TJ ET EMC /P <</MCID 109 >> BDC BT 1 0 0 -1 0 51.09700012 Tm [(1%)] TJ ET EMC /P <</MCID 110 >> BDC BT 1 0 0 -1 13.28499985 51.09700012 Tm [( )] TJ ET EMC /P <</MCID 111 >> BDC BT 1 0 0 -1 17.78499985 51.09700012 Tm [(SDS\).)] TJ ET EMC /P 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<</MCID 124 >> BDC BT 1 0 0 -1 165.35400391 51.09700012 Tm [(wa)1(s)] TJ ET EMC /P <</MCID 125 >> BDC BT 1 0 0 -1 181.60099792 51.09700012 Tm [( )] TJ ET EMC /P <</MCID 126 >> BDC BT 1 0 0 -1 186.10099792 51.09700012 Tm [(mixed)] TJ ET EMC /P <</MCID 127 >> BDC BT 1 0 0 -1 212.35400391 51.09700012 Tm [( )] TJ ET EMC /P <</MCID 128 >> BDC BT 1 0 0 -1 216.85400391 51.09700012 Tm [(wit)1(h)] TJ ET EMC /P <</MCID 129 >> BDC BT 1 0 0 -1 235.25 51.09700012 Tm [( )] TJ ET EMC /P <</MCID 130 >> BDC BT 1 0 0 -1 239.75 51.09700012 Tm [(4)] TJ ET EMC /P <</MCID 131 >> BDC BT 1 0 0 -1 245.02799988 51.09700012 Tm [( )] TJ ET EMC /P <</MCID 132 >> BDC BT 1 0 0 -1 249.52799988 51.09700012 Tm [(times)] TJ ET EMC /P <</MCID 133 >> BDC BT 1 0 0 -1 272.98199463 51.09700012 Tm [( )] TJ ET EMC /P <</MCID 134 >> BDC BT 1 0 0 -1 277.48199463 51.09700012 Tm [(volume)] TJ ET EMC /P <</MCID 135 >> BDC BT 1 0 0 -1 309.4079895 51.09700012 Tm [( )] TJ ET EMC /P <</MCID 136 >> BDC BT 1 0 0 -1 313.9079895 51.09700012 Tm [(of)] TJ ET EMC /P <</MCID 137 >> BDC BT 1 0 0 -1 322.75 51.09700012 Tm [( )] TJ ET EMC /P <</MCID 138 >> BDC BT 1 0 0 -1 327.25 51.09700012 Tm [(5X)] TJ ET EMC /P <</MCID 139 >> BDC BT 1 0 0 -1 338.33300781 51.09700012 Tm [( )] TJ ET EMC /P <</MCID 140 >> BDC BT 1 0 0 -1 342.83300781 51.09700012 Tm [(S)] TJ ET EMC /P <</MCID 141 >> BDC BT 1 0 0 -1 0 66.6969986 Tm [(DS-)1(PAGE)] TJ ET EMC /P <</MCID 142 >> BDC BT 1 0 0 -1 39.42300034 66.6969986 Tm [( )] TJ ET EMC /P <</MCID 143 >> BDC BT 1 0 0 -1 43.92300034 66.6969986 Tm [(load)1(in)-1(g)] TJ ET EMC /P <</MCID 144 >> BDC BT 1 0 0 -1 76.46900177 66.6969986 Tm [( )] TJ ET EMC /P <</MCID 145 >> BDC BT 1 0 0 -1 80.96900177 66.6969986 Tm [(buff)1(e)-1(r)1(.)] TJ ET EMC /P <</MCID 146 >> BDC BT 1 0 0 -1 109.21299744 66.6969986 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 0 0.75 m 0 95.09999847 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 64.05000305 0.75 m 64.05000305 95.09999847 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 422.79998779 0.75 m 422.79998779 95.09999847 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d -0.375 0.375 m 423.17498779 0.375 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d -0.375 95.47499847 m 423.17498779 95.47499847 l S Q Q q 1 0 0 1 0 460.82299805 cm /P <</MCID 147 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 488.76199341 cm /P <</MCID 148 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 516.70098877 cm /P <</MCID 149 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 544.64001465 cm /P <</MCID 150 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 572.57897949 cm Q Q 1 0 0 -1 0 841.90002441 cm 
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