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135.95199585 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 113.87799835 cm /P <</Lang (zh-CN) /MCID 16 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 12.82499981 Tm /GS2 gs 0 g [<000E>] TJ ET EMC /P <</MCID 17 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 10.5 12.82499981 Tm [(    )] TJ ET EMC /P <</Lang (zh-CN) /MCID 18 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 31.5 12.82499981 Tm [<000A000C>] TJ ET EMC /P <</MCID 19 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 52.5 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</MCID 20 >> BDC BT 1 0 0 -1 63 12.82499981 Tm /GS2 gs 0 g [(HT-)-1(2)1(9)] TJ ET EMC /P <</Lang (zh-CN) /MCID 21 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 91.14199829 12.82499981 Tm [<00030004000500060007>] TJ ET EMC /P <</MCID 22 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 143.64199829 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 147.41700745 cm /P <</Lang (zh-CN) /MCID 23 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 12.82499981 Tm /GS2 gs 0 g [<000F00100011000E000C>] TJ ET EMC /P <</MCID 24 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 52.5 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 25 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 63 12.82499981 Tm /GS2 gs 0 g [<00120013>] TJ ET EMC /P <</MCID 26 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 84 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</MCID 27 >> BDC BT 1 0 0 -1 105 12.82499981 Tm [( )] TJ ET EMC /P <</MCID 28 >> BDC BT 1 0 0 -1 0 30.49699783 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 29 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 48.70299911 Tm /GS2 gs 0 g [<0014>] TJ ET EMC /P <</MCID 30 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 10.5 48.70299911 Tm [(    )] TJ ET EMC /P <</Lang (zh-CN) /MCID 31 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 31.5 48.70299911 Tm [<0015000C>] TJ ET EMC /P <</MCID 32 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 52.5 48.70299911 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 33 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 63 48.70299911 Tm /GS2 gs 0 g [<001600170018>] TJ ET EMC /P <</MCID 34 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 94.5 48.70299911 Tm [(-20)] TJ ET EMC /P <</Lang (zh-CN) /MCID 35 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 108.35800171 48.70299911 Tm [<0019001A>] TJ ET EMC /P <</MCID 36 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 129.35800171 48.70299911 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 37 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 134.60800171 48.70299911 Tm [<001B001C>] TJ ET EMC /P <</MCID 38 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 155.60800171 48.70299911 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q /GS2 gs 1 g -1.45000005 216.83399963 m 416.75 216.83399963 l 416.75 248.03399658 l -1.45000005 248.03399658 l h f* Q q 1 0 0 1 0 216.83399963 cm /P <</MCID 39 >> BDC q /GS2 gs 1 g 0 6.59399986 m 21 6.59399986 l 21 24.60499954 l 0 24.60499954 l h f* Q EMC /P <</Lang (zh-CN) /MCID 40 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 20.17499924 Tm /GS2 gs 0.13333334 g [<00030004>] TJ ET EMC /P <</MCID 41 >> BDC BT /FAAACJ 10.5 Tf 1 0 0 -1 21 20.17499924 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 248.03399658 cm /P <</MCID 42 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 -5.4000001 275.97299194 cm q -0.75 -0.5 m 426.8500061 -0.5 l 426.8500061 29.43899918 l -0.75 29.43899918 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 43 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm /GS2 gs 0 g [(HT-)-1(2)1(9)] TJ ET EMC /P <</MCID 44 >> BDC BT 1 0 0 -1 25.5170002 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 45 >> BDC BT 1 0 0 -1 27.89100075 12.55799961 Tm [(ce)1(l)-1(l)] TJ ET EMC /P <</MCID 46 >> BDC BT 1 0 0 -1 42.375 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 47 >> BDC BT 1 0 0 -1 44.74900055 12.55799961 Tm [(lysates)] TJ ET EMC /P <</MCID 48 >> BDC BT 1 0 0 -1 73.89600372 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 49 >> BDC BT 1 0 0 -1 76.26999664 12.55799961 Tm [(from)] TJ ET EMC /P <</MCID 50 >> BDC BT 1 0 0 -1 97.05999756 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 51 >> BDC BT 1 0 0 -1 99.43399811 12.55799961 Tm [(hu)-1(man)] TJ ET EMC /P <</MCID 52 >> BDC BT 1 0 0 -1 129.40100098 12.55799961 Tm [( )] TJ ET EMC /P 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281.70300293 12.55799961 Tm [(the)] TJ ET EMC /P <</MCID 66 >> BDC BT 1 0 0 -1 295.96099854 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 67 >> BDC BT 1 0 0 -1 298.33499146 12.55799961 Tm [(me)1(d)-1(ia)] TJ ET EMC /P <</MCID 68 >> BDC BT 1 0 0 -1 324.90301514 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 69 >> BDC BT 1 0 0 -1 327.27700806 12.55799961 Tm [(con)-1(taini)-1(ng)] TJ ET EMC /P <</MCID 70 >> BDC BT 1 0 0 -1 372.11199951 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 71 >> BDC BT 1 0 0 -1 374.48599243 12.55799961 Tm [(10%)] TJ ET EMC /P <</MCID 72 >> BDC BT 1 0 0 -1 392.63500977 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 73 >> BDC BT 1 0 0 -1 395.00900269 12.55799961 Tm [(FBS)-1(.)] TJ ET EMC /P <</MCID 74 >> BDC BT /FAAACJ 10.5 Tf 1 0 0 -1 413.01998901 12.55799961 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 0.5 w 0 0.5 m 0 28.43899918 l S Q q 0.5 w 426.1000061 0.5 m 426.1000061 28.43899918 l S Q q 0.5 w -0.25 0.25 m 426.3500061 0.25 l S Q q 0.5 w -0.25 28.68899918 m 426.3500061 28.68899918 l S 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0 -1 72.02600098 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 88 >> BDC BT 1 0 0 -1 76.52600098 11.72200012 Tm [(in)] TJ ET EMC /P <</MCID 89 >> BDC BT 1 0 0 -1 84.46700287 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 90 >> BDC BT 1 0 0 -1 88.96700287 11.72200012 Tm [(lysis)] TJ ET EMC /P <</MCID 91 >> BDC BT 1 0 0 -1 106.85299683 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 92 >> BDC BT 1 0 0 -1 111.35299683 11.72200012 Tm [(buff)1(e)-1(r)] TJ ET EMC /P <</MCID 93 >> BDC BT 1 0 0 -1 137.42999268 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 94 >> BDC BT 1 0 0 -1 141.92999268 11.72200012 Tm [(\(50)-1(mM)] TJ ET EMC /P <</MCID 95 >> BDC BT 1 0 0 -1 172.71800232 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 96 >> BDC BT 1 0 0 -1 177.21800232 11.72200012 Tm [(Tris\(PH7)-1(.)1(4)-1(\),)] TJ ET EMC /P <</MCID 97 >> BDC BT 1 0 0 -1 225.74200439 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 98 >> BDC BT 1 0 0 -1 230.24200439 11.72200012 Tm [(15)-1(0mM)] TJ ET EMC /P <</MCID 99 >> BDC BT 1 0 0 -1 263.30200195 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203.32299805 27.3220005 Tm [(mixed)] TJ ET EMC /P <</MCID 125 >> BDC BT 1 0 0 -1 229.57600403 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 126 >> BDC BT 1 0 0 -1 234.07600403 27.3220005 Tm [(wit)1(h)] TJ ET EMC /P <</MCID 127 >> BDC BT 1 0 0 -1 252.47200012 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 128 >> BDC BT 1 0 0 -1 256.97198486 27.3220005 Tm [(4)] TJ ET EMC /P <</MCID 129 >> BDC BT 1 0 0 -1 262.25 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 130 >> BDC BT 1 0 0 -1 266.75 27.3220005 Tm [(times)] TJ ET EMC /P <</MCID 131 >> BDC BT 1 0 0 -1 290.20401001 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 132 >> BDC BT 1 0 0 -1 294.70401001 27.3220005 Tm [(volume)] TJ ET EMC /P <</MCID 133 >> BDC BT 1 0 0 -1 326.63000488 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 134 >> BDC BT 1 0 0 -1 0 42.92200089 Tm [(of)] TJ ET EMC /P <</MCID 135 >> BDC BT 1 0 0 -1 8.84200001 42.92200089 Tm [( )] TJ ET EMC /P <</MCID 136 >> BDC BT 1 0 0 -1 13.34200001 42.92200089 Tm [(5X)] TJ ET EMC /P <</MCID 137 >> BDC BT 1 0 0 -1 24.42499924 42.92200089 Tm [( )] TJ ET EMC /P <</MCID 138 >> BDC BT 1 0 0 -1 28.92499924 42.92200089 Tm [(SDS-P)1(AGE)] TJ ET EMC /P <</MCID 139 >> BDC BT 1 0 0 -1 73.54299927 42.92200089 Tm [( )] TJ ET EMC /P <</MCID 140 >> BDC BT 1 0 0 -1 78.04299927 42.92200089 Tm [(load)1(in)-1(g)] TJ ET EMC /P <</MCID 141 >> BDC BT 1 0 0 -1 110.58899689 42.92200089 Tm [( )] TJ ET EMC /P <</MCID 142 >> BDC BT 1 0 0 -1 115.08899689 42.92200089 Tm [(buff)1(e)-1(r)1(.)] TJ ET EMC /P <</MCID 143 >> BDC BT 1 0 0 -1 143.33299255 42.92200089 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 0 0.75 m 0 47.54999924 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 82.40000153 0.75 m 82.40000153 47.54999924 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 422.79998779 0.75 m 422.79998779 47.54999924 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d -0.375 0.375 m 423.17498779 0.375 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d -0.375 47.92499924 m 423.17498779 47.92499924 l S Q Q q 1 0 0 1 0 443.55099487 cm /P <</MCID 144 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 471.48999023 cm /P <</MCID 145 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 499.4289856 cm /P <</MCID 146 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 527.36798096 cm /P <</MCID 147 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 555.30700684 cm Q Q 1 0 0 -1 0 841.90002441 cm 
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