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12.82499981 Tm [( )] TJ ET EMC /P <</MCID 16 >> BDC BT 1 0 0 -1 133.69599915 12.82499981 Tm [(Lysate)] TJ ET EMC /P <</MCID 17 >> BDC BT 1 0 0 -1 160.74099731 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 113.87799835 cm /P <</Lang (zh-CN) /MCID 18 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 12.82499981 Tm /GS2 gs 0 g [<000E>] TJ ET EMC /P <</MCID 19 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 10.5 12.82499981 Tm [(    )] TJ ET EMC /P <</Lang (zh-CN) /MCID 20 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 31.5 12.82499981 Tm [<000A000C>] TJ ET EMC /P <</MCID 21 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 52.5 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</MCID 22 >> BDC BT 1 0 0 -1 63 12.82499981 Tm /GS2 gs 0 g [(HEPG2.2.)] TJ ET EMC /P <</MCID 23 >> BDC BT 1 0 0 -1 102.66200256 12.82499981 Tm [(15)] TJ ET EMC /P <</Lang (zh-CN) /MCID 24 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 115.93099976 12.82499981 Tm [<00030004000500060007>] TJ ET EMC /P <</MCID 25 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 168.43099976 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 147.41700745 cm /P <</Lang (zh-CN) /MCID 26 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 12.82499981 Tm /GS2 gs 0 g [<000F00100011000E000C>] TJ ET EMC /P <</MCID 27 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 52.5 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 28 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 63 12.82499981 Tm /GS2 gs 0 g [<00120013>] TJ ET EMC /P <</MCID 29 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 84 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</MCID 30 >> BDC BT 1 0 0 -1 105 12.82499981 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 180.95599365 cm /P <</Lang (zh-CN) /MCID 31 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 0 12.82499981 Tm /GS2 gs 0 g [<0014>] TJ ET EMC /P <</MCID 32 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 10.5 12.82499981 Tm [(    )] TJ ET EMC /P <</Lang (zh-CN) /MCID 33 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 31.5 12.82499981 Tm [<0015000C>] TJ ET EMC /P <</MCID 34 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 52.5 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 35 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 63 12.82499981 Tm /GS2 gs 0 g [<001600170018>] TJ ET EMC /P <</MCID 36 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 94.5 12.82499981 Tm [(-20)] TJ ET EMC /P <</Lang (zh-CN) /MCID 37 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 108.35800171 12.82499981 Tm [<0019001A>] TJ ET EMC /P <</MCID 38 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 129.35800171 12.82499981 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 39 >> BDC BT /FAAABJ 10.5 Tf 1 0 0 -1 134.60800171 12.82499981 Tm [<001B001C>] TJ ET EMC /P <</MCID 40 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 155.60800171 12.82499981 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q /GS2 gs 1 g -1.45000005 214.49499512 m 416.75 214.49499512 l 416.75 245.69499207 l -1.45000005 245.69499207 l h f* Q q 1 0 0 1 0 214.49499512 cm /P <</MCID 41 >> BDC q /GS2 gs 1 g 0 6.59399986 m 21 6.59399986 l 21 24.60499954 l 0 24.60499954 l h f* Q EMC /P <</Lang (zh-CN) /MCID 42 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 0 20.17499924 Tm /GS2 gs 0.13333334 g [<00030004>] TJ ET EMC /P <</MCID 43 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 21 20.17499924 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 245.69500732 cm /P <</MCID 44 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 -5.4000001 273.63400269 cm q -0.75 -0.5 m 426.8500061 -0.5 l 426.8500061 47.37799835 l -0.75 47.37799835 l h W n q 1 0 0 1 5.4000001 0.5 cm /P <</MCID 45 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm /GS2 gs 0 g [(HEPG2.2.15)] TJ ET EMC /P <</MCID 46 >> BDC BT 1 0 0 -1 50.30599976 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 47 >> BDC BT 1 0 0 -1 53.31399918 12.55799961 Tm [(ce)1(l)-1(l)] TJ ET EMC /P <</MCID 48 >> BDC BT 1 0 0 -1 67.79799652 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 49 >> BDC BT 1 0 0 -1 70.80599976 12.55799961 Tm [(lysates)] TJ ET EMC /P <</MCID 50 >> BDC BT 1 0 0 -1 99.95300293 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 51 >> BDC BT 1 0 0 -1 102.96099854 12.55799961 Tm [(from)] TJ ET EMC /P <</MCID 52 >> BDC BT 1 0 0 -1 123.75099945 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 53 >> BDC BT 1 0 0 -1 126.75900269 12.55799961 Tm [(hu)-1(man)] TJ ET EMC /P <</MCID 54 >> BDC BT 1 0 0 -1 156.72599792 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 55 >> BDC BT 1 0 0 -1 159.73399353 12.55799961 Tm [(hepato)-1(m)1(a)] TJ ET EMC /P <</MCID 56 >> BDC BT 1 0 0 -1 203.49299622 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 57 >> BDC BT 1 0 0 -1 206.50100708 12.55799961 Tm [(ce)1(l)-1(ls)] TJ ET EMC /P <</MCID 58 >> BDC BT 1 0 0 -1 225.09100342 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 59 >> BDC BT 1 0 0 -1 228.09899902 12.55799961 Tm [(we)1(re)] TJ ET EMC /P <</MCID 60 >> BDC BT 1 0 0 -1 249.71400452 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 61 >> BDC BT 1 0 0 -1 252.72200012 12.55799961 Tm [(cul)-1(ture)1(d)] TJ ET EMC /P <</MCID 62 >> BDC BT 1 0 0 -1 288.52398682 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 63 >> BDC BT 1 0 0 -1 291.53201294 12.55799961 Tm [(in)] TJ ET EMC /P <</MCID 64 >> BDC BT 1 0 0 -1 299.45800781 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 65 >> BDC BT 1 0 0 -1 302.46600342 12.55799961 Tm [(the)] TJ ET EMC /P <</MCID 66 >> BDC BT 1 0 0 -1 316.72399902 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 67 >> BDC BT 1 0 0 -1 319.73199463 12.55799961 Tm [(me)1(d)-1(ia)] TJ ET EMC /P <</MCID 68 >> BDC BT 1 0 0 -1 346.29998779 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 69 >> BDC BT 1 0 0 -1 349.30801392 12.55799961 Tm [(con)-1(taini)-1(ng)] TJ ET EMC /P <</MCID 70 >> BDC BT 1 0 0 -1 394.14300537 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 71 >> BDC BT 1 0 0 -1 397.15100098 12.55799961 Tm [(20%)] TJ ET EMC /P <</MCID 72 >> BDC BT 1 0 0 -1 415.29998779 12.55799961 Tm [( )] TJ ET EMC /P <</MCID 73 >> BDC BT 1 0 0 -1 0 30.49699783 Tm [(FBS)-1(.)] TJ ET EMC /P <</MCID 74 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 18.01099968 30.49699783 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q 0.5 w 0 0.5 m 0 46.37799835 l S Q q 0.5 w 426.1000061 0.5 m 426.1000061 46.37799835 l S Q q 0.5 w -0.25 0.25 m 426.3500061 0.25 l S Q q 0.5 w -0.25 46.62799835 m 426.3500061 46.62799835 l S Q Q q /GS2 gs 1 g -1.45000005 320.51199341 m 416.75 320.51199341 l 416.75 351.71200562 l -1.45000005 351.71200562 l h f* Q q /GS2 gs 1 g -1.45000005 351.71200562 m 416.75 351.71200562 l 416.75 382.91201782 l -1.45000005 382.91201782 l h f* Q q 1 0 0 1 0 320.51199341 cm /P <</MCID 75 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 0 20.17499924 Tm [( )] TJ ET EMC /P <</MCID 76 >> BDC q /GS2 gs 1 g 0 37.79399872 m 42 37.79399872 l 42 55.80500031 l 0 55.80500031 l h f* Q EMC /P <</Lang (zh-CN) /MCID 77 >> BDC BT /FAAACH 10.5 Tf 1 0 0 -1 0 51.375 Tm /GS2 gs 0.13333334 g [<0005000600070008>] TJ ET EMC /P <</MCID 78 >> BDC BT /FAAACF 10.5 Tf 1 0 0 -1 42 51.375 Tm /GS1 gs 0 g [( )] TJ ET EMC Q q 1 0 0 1 0 382.9119873 cm /P <</MCID 79 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 -3.75 410.85101318 cm q /GS2 gs 1 g 0.375 0.75 m 76.97499847 0.75 l 76.97499847 47.54999924 l 0.375 47.54999924 l h f* Q q -0.625 -0.25 m 77.97499847 -0.25 l 77.97499847 48.54999924 l -0.625 48.54999924 l h W n q 1 0 0 1 3.75 0.75 cm /P <</MCID 80 >> BDC BT /FAAACF 9 Tf 1 0 0 -1 0 27.3220005 Tm /GS2 gs 0.13333334 g [(Ba)1(ckgr)1(o)-1(und)] TJ ET EMC /P <</MCID 81 >> BDC BT /FAAADC 9 Tf 1 0 0 -1 54.72100067 27.3220005 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q /GS2 gs 1 g 77.72499847 0.75 m 422.42501831 0.75 l 422.42501831 47.54999924 l 77.72499847 47.54999924 l h f* Q q 1 0 0 1 77.34999847 0 cm -0.625 -0.25 m 346.07501221 -0.25 l 346.07501221 48.54999924 l -0.625 48.54999924 l h W n q 1 0 0 1 3.75 0.75 cm /P <</MCID 82 >> BDC BT /FAAADC 9 Tf 1 0 0 -1 0 11.72200012 Tm /GS2 gs 0.13333334 g [(Cell)-1(s)] TJ ET EMC /P <</MCID 83 >> BDC BT 1 0 0 -1 20.08300018 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 84 >> BDC BT 1 0 0 -1 24.58300018 11.72200012 Tm [(were)] TJ ET EMC /P <</MCID 85 >> BDC BT 1 0 0 -1 45.33800125 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 86 >> BDC BT 1 0 0 -1 49.83800125 11.72200012 Tm [(lyse)-1(d)] TJ ET EMC /P <</MCID 87 >> BDC BT 1 0 0 -1 72.02600098 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 88 >> BDC BT 1 0 0 -1 76.52600098 11.72200012 Tm [(in)] TJ ET EMC /P <</MCID 89 >> BDC BT 1 0 0 -1 84.46700287 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 90 >> BDC BT 1 0 0 -1 88.96700287 11.72200012 Tm [(lysis)] TJ ET EMC /P <</MCID 91 >> BDC BT 1 0 0 -1 106.85299683 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 92 >> BDC BT 1 0 0 -1 111.35299683 11.72200012 Tm [(buff)1(e)-1(r)] TJ ET EMC /P <</MCID 93 >> BDC BT 1 0 0 -1 137.42999268 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 94 >> BDC BT 1 0 0 -1 141.92999268 11.72200012 Tm [(\(50)-1(mM)] TJ ET EMC /P <</MCID 95 >> BDC BT 1 0 0 -1 172.71800232 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 96 >> BDC BT 1 0 0 -1 177.21800232 11.72200012 Tm [(Tris\(PH7)-1(.)1(4)-1(\),)] TJ ET EMC /P <</MCID 97 >> BDC BT 1 0 0 -1 225.74200439 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 98 >> BDC BT 1 0 0 -1 230.24200439 11.72200012 Tm [(15)-1(0mM)] TJ ET EMC /P <</MCID 99 >> BDC BT 1 0 0 -1 263.30200195 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 100 >> BDC BT 1 0 0 -1 267.80200195 11.72200012 Tm [(NaCl,)] TJ ET EMC /P <</MCID 101 >> BDC BT 1 0 0 -1 290.67599487 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 102 >> BDC BT 1 0 0 -1 295.17599487 11.72200012 Tm [(1%)] TJ ET EMC /P <</MCID 103 >> BDC BT 1 0 0 -1 308.46099854 11.72200012 Tm [( )] TJ ET EMC /P <</MCID 104 >> BDC BT 1 0 0 -1 312.96099854 11.72200012 Tm [(NP-)1(4)] TJ ET EMC /P <</MCID 105 >> BDC BT 1 0 0 -1 0 27.3220005 Tm [(0,)] TJ ET EMC /P <</MCID 106 >> BDC BT 1 0 0 -1 7.44399977 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 107 >> BDC BT 1 0 0 -1 11.94400024 27.3220005 Tm [(1%)] TJ ET EMC /P <</MCID 108 >> BDC BT 1 0 0 -1 25.22900009 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 109 >> BDC BT 1 0 0 -1 29.72900009 27.3220005 Tm [(SDS\).)] TJ ET EMC /P <</MCID 110 >> BDC BT 1 0 0 -1 52.14599991 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 111 >> BDC BT 1 0 0 -1 56.64599991 27.3220005 Tm [(10)-1(0)] TJ ET EMC /P <</MCID 112 >> BDC BT 1 0 0 -1 72.47899628 27.3220005 Tm [( )] TJ ET EMC /P <</Lang (zh-CN) /MCID 113 >> BDC BT /FAAADE 9 Tf 1 0 0 -1 76.97899628 27.3220005 Tm [<0024>] TJ ET EMC /P <</MCID 114 >> BDC BT /FAAADC 9 Tf 1 0 0 -1 82.62999725 27.3220005 Tm [(g)] TJ ET EMC /P <</MCID 115 >> BDC BT 1 0 0 -1 88.38700104 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 116 >> BDC BT 1 0 0 -1 92.88700104 27.3220005 Tm [(of)] TJ ET EMC /P <</MCID 117 >> BDC BT 1 0 0 -1 101.72899628 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 118 >> BDC BT 1 0 0 -1 106.22899628 27.3220005 Tm [(ce)-1(llular)] TJ ET EMC /P <</MCID 119 >> BDC BT 1 0 0 -1 136.99099731 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 120 >> BDC BT 1 0 0 -1 141.49099731 27.3220005 Tm [(pro)1(tein)] TJ ET EMC /P <</MCID 121 >> BDC BT 1 0 0 -1 172.79800415 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 122 >> BDC BT 1 0 0 -1 177.29800415 27.3220005 Tm [(wa)1(s)] TJ ET EMC /P <</MCID 123 >> BDC BT 1 0 0 -1 193.54499817 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 124 >> BDC BT 1 0 0 -1 198.04499817 27.3220005 Tm [(mixed)] TJ ET EMC /P <</MCID 125 >> BDC BT 1 0 0 -1 224.29800415 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 126 >> BDC BT 1 0 0 -1 228.79800415 27.3220005 Tm [(wit)1(h)] TJ ET EMC /P <</MCID 127 >> BDC BT 1 0 0 -1 247.19400024 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 128 >> BDC BT 1 0 0 -1 251.69400024 27.3220005 Tm [(4)] TJ ET EMC /P <</MCID 129 >> BDC BT 1 0 0 -1 256.97198486 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 130 >> BDC BT 1 0 0 -1 261.47198486 27.3220005 Tm [(times)] TJ ET EMC /P <</MCID 131 >> BDC BT 1 0 0 -1 284.92599487 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 132 >> BDC BT 1 0 0 -1 289.42599487 27.3220005 Tm [(volume)] TJ ET EMC /P <</MCID 133 >> BDC BT 1 0 0 -1 321.35198975 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 134 >> BDC BT 1 0 0 -1 325.85198975 27.3220005 Tm [(of)] TJ ET EMC /P <</MCID 135 >> BDC BT 1 0 0 -1 334.69400024 27.3220005 Tm [( )] TJ ET EMC /P <</MCID 136 >> BDC BT 1 0 0 -1 0 42.92200089 Tm [(5X)] TJ ET EMC /P <</MCID 137 >> BDC BT 1 0 0 -1 11.08300018 42.92200089 Tm [( )] TJ ET EMC /P <</MCID 138 >> BDC BT 1 0 0 -1 15.58300018 42.92200089 Tm [(SDS-P)1(AGE)] TJ ET EMC /P <</MCID 139 >> BDC BT 1 0 0 -1 60.20100021 42.92200089 Tm [( )] TJ ET EMC /P <</MCID 140 >> BDC BT 1 0 0 -1 64.7009964 42.92200089 Tm [(load)1(in)-1(g)] TJ ET EMC /P <</MCID 141 >> BDC BT 1 0 0 -1 97.24700165 42.92200089 Tm [( )] TJ ET EMC /P <</MCID 142 >> BDC BT 1 0 0 -1 101.74700165 42.92200089 Tm [(buff)1(e)-1(r)1(.)] TJ ET EMC /P <</MCID 143 >> BDC BT 1 0 0 -1 129.99099731 42.92200089 Tm /GS1 gs 0 g [( )] TJ ET EMC Q Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 0 0.75 m 0 47.54999924 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 77.34999847 0.75 m 77.34999847 47.54999924 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d 422.80001831 0.75 m 422.80001831 47.54999924 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d -0.375 0.375 m 423.17501831 0.375 l S Q q /GS1 gs 0.75686276 G 0.75 w [0.75 0.75] 0 d -0.375 47.92499924 m 423.17501831 47.92499924 l S Q Q q 1 0 0 1 0 459.15100098 cm /P <</MCID 144 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 487.08999634 cm /P <</MCID 145 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 515.0289917 cm /P <</MCID 146 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 542.96801758 cm /P <</MCID 147 >> BDC BT /FAAAAH 10.5 Tf 1 0 0 -1 0 12.55799961 Tm [( )] TJ ET EMC Q q 1 0 0 1 0 570.90698242 cm Q Q 1 0 0 -1 0 841.90002441 cm 
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