ELISA試劑盒分析儀器

<p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">技術(shù)</font><font face="Calibri">20</font><font face="宋體">世紀(jì)</font><font face="Calibri">80</font><font face="宋體">年代引入中國(guó)，現(xiàn)已被廣泛應(yīng)用。與之相關(guān)的分析儀器也得到了快速發(fā)展，我國(guó)先后出現(xiàn)了十余家酶標(biāo)儀、洗板機(jī)生產(chǎn)廠商，在中國(guó)市場(chǎng)上與眾多的進(jìn)口酶標(biāo)儀器進(jìn)行緊張競(jìng)爭(zhēng)。下面分三個(gè)方面對(duì)</font><font face="Calibri">ELISA</font><font face="宋體">中有關(guān)儀器及其在中國(guó)的應(yīng)用作一簡(jiǎn)要介紹。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">分離式酶聯(lián)免疫儀器</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">分離式酶聯(lián)免疫儀器主要有酶標(biāo)儀、洗板機(jī)。在血清離心之后，經(jīng)過加樣、洗板、孵育等過程，最后通過吸光度判定結(jié)果。</font>ELISA<font face="宋體">實(shí)驗(yàn)在國(guó)內(nèi)主要用于定性檢驗(yàn)，故目前的分離式儀器基本上可以滿足。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">酶標(biāo)儀本質(zhì)上是一臺(tái)專用于微孔板的、可見光范圍的光電比色計(jì)。在</font>ELISA<font face="宋體">實(shí)驗(yàn)中，酶標(biāo)儀是測(cè)定各微孔中試樣的吸光度的關(guān)鍵儀器，對(duì)試驗(yàn)結(jié)果的判定有直接影響，是實(shí)驗(yàn)室必備的重要儀器之一。隨著</font><font face="Calibri">ELISA</font><font face="宋體">技術(shù)的普及，酶標(biāo)儀得到了很大發(fā)展，產(chǎn)品種類齊全，型號(hào)多樣，各有特色。目前國(guó)內(nèi)市場(chǎng)上銷售的酶標(biāo)儀已完全擺脫原來的單孔測(cè)量方式，整板測(cè)量、定性定量計(jì)算、存儲(chǔ)、多種報(bào)告格式、濾光片自動(dòng)轉(zhuǎn)換等已成為基本的、必備的功能，各種新推出的酶標(biāo)儀性能日益完善，功能不斷擴(kuò)充。具體有：</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">測(cè)量高速化 從目前不斷推出的酶標(biāo)儀趨勢(shì)來看，整板測(cè)量速度均在</font><font face="Calibri">10s</font><font face="宋體">以內(nèi)，這有利于防止測(cè)量過程中各微孔吸光度的微小變化，盡可能克服環(huán)境對(duì)結(jié)果的影響，同時(shí)可以滿足動(dòng)力測(cè)量的要求，測(cè)量速度在</font><font face="Calibri">20s</font><font face="宋體">以上的儀器將處于不利于地位。我國(guó)現(xiàn)生產(chǎn)的酶標(biāo)儀，測(cè)量整板速度一般為</font><font face="Calibri">25s,</font><font face="宋體">產(chǎn)品更新周期較長(zhǎng)。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">寬吸光度范圍</font> <font face="宋體">盡管酶標(biāo)儀</font>A=0-2.5<font face="宋體">的吸光度范圍完全可以滿足實(shí)驗(yàn)的要求，但國(guó)外新推出的儀器都擴(kuò)寬了早期型號(hào)的吸光度范圍，較高級(jí)的酶標(biāo)儀其線性范圍可以達(dá)到</font><font face="Calibri">A=4.0</font><font face="宋體">，并且保持很好的精密度，如</font><font face="Calibri">BIORAD</font><font face="宋體">公司的</font><font face="Calibri">Benchmark,</font><font face="宋體">在</font><font face="Calibri">400-750nm</font><font face="宋體">、</font><font face="Calibri">A=0-4.0</font><font face="宋體">時(shí)其重復(fù)性與線性均不大于</font><font face="Calibri">3.5%</font><font face="宋體">。又如</font><font face="Calibri">LABYSTEMS</font><font face="宋體">公司的</font><font face="Calibri">Multi-skan Ascent,</font><font face="宋體">其吸光度讀數(shù)范圍達(dá)</font><font face="Calibri">A=0-4.0</font><font face="宋體">，在</font><font face="Calibri">A=3.0-4.0</font><font face="宋體">范圍，其線性不超過</font><font face="Calibri">2%, RSD</font><font face="宋體">小于</font><font face="Calibri">1.0%</font><font face="宋體">。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">擴(kuò)展到紫外</font> <font face="宋體">增加紫外濾光片幾乎是廠商的共同選擇。由于各種酶標(biāo)儀都配有放置濾光片的可自動(dòng)轉(zhuǎn)換的機(jī)構(gòu)，可以同時(shí)安裝</font>6-8<font face="宋體">片濾光片，擴(kuò)展紫外功能相對(duì)容易，一般都增加</font><font face="Calibri">340nm</font><font face="宋體">濾光片。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">具有孵育功能 可以自動(dòng)、精確控制反應(yīng)溫度，使微孔板的孵育過程在儀器內(nèi)部完成，降低外界干擾，簡(jiǎn)化操作步驟。如</font><font face="Calibri">BIORAD</font><font face="宋體">公司的</font><font face="Calibri">Benchmark,</font><font face="宋體">芬蘭</font><font face="Calibri">LABSYSTEMS</font><font face="宋體">公司的</font><font face="Calibri">Multiskan Ascent,BIOTCK</font><font face="宋體">公司的</font><font face="Calibri">EIX808</font><font face="宋體">等。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">具有動(dòng)力學(xué)檢測(cè)功能</font> <font face="宋體">常規(guī)</font>ELISA<font face="宋體">是一種終點(diǎn)法 測(cè)量實(shí)驗(yàn)，測(cè)量前需要加入終止液來抑制酶免反應(yīng)的進(jìn)行，通過吸光度的大小來計(jì)算待測(cè)物質(zhì)的濃度，動(dòng)力學(xué)方法是通過測(cè)定反應(yīng)過程中吸光度的變化速率來計(jì)算相應(yīng)的待測(cè)物質(zhì)的含量的，酶標(biāo)儀的測(cè)量速度加快后，只要增加相應(yīng)的軟件，就可以使用酶標(biāo)儀完成某些生化指標(biāo)的動(dòng)力學(xué)測(cè)量。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>