今天與您分享培育基的注意事項(xiàng)

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">在運(yùn)用培育基的時(shí)候有很多細(xì)節(jié)是需要注意的，近來(lái)，也有不少客戶過(guò)培育基的相關(guān)事宜</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">我司技術(shù)部將培育基的注意事項(xiàng)整理分析如下：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">切記，細(xì)胞培育基的儲(chǔ)存條件是</font>2-8<font face="宋體">攝氏度。假如細(xì)胞培育基不小心被凍，您應(yīng)該消融培育基并觀察是否有沉積發(fā)生。假如沒(méi)有沉積發(fā)生，培育基能夠正常運(yùn)用，假如出現(xiàn)沉積，只能丟棄這些培育基。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">在進(jìn)行傳代培育時(shí)，咱們強(qiáng)烈引薦進(jìn)行臺(tái)盼蘭活性記數(shù)。研究者常常通過(guò)一個(gè)簡(jiǎn)單的稀釋</font>(1<font face="宋體">∶</font><font face="Calibri">4</font><font face="宋體">或</font><font face="Calibri">1</font><font face="宋體">∶</font><font face="Calibri">2)</font><font face="宋體">進(jìn)行傳代，不進(jìn)行活性檢測(cè)，您或許接種比你認(rèn)為的低的多的濃度的細(xì)胞，這常?；蛟S導(dǎo)致成長(zhǎng)緩慢或培育物根本不成長(zhǎng)。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">儲(chǔ)存在</font>4<font face="宋體">℃冰箱中的液體胰蛋白酶溶液只能運(yùn)用一周。胰蛋白酶在</font><font face="Calibri">4</font><font face="宋體">℃就或許開(kāi)端降解，假如在室溫下放置超越</font><font face="Calibri">30</font><font face="宋體">分鐘，就會(huì)變得不穩(wěn)定。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">在訂貨的細(xì)胞抵達(dá)后，應(yīng)立即復(fù)蘇，假如培育基還沒(méi)有準(zhǔn)備好，可將其放入液氮中，趕快復(fù)蘇。千萬(wàn)不能放置在</font>-20<font face="宋體">或</font><font face="Calibri">-80</font><font face="宋體">℃的冰箱內(nèi)。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">細(xì)胞復(fù)蘇往往是比較簡(jiǎn)單出問(wèn)題的過(guò)程。請(qǐng)?jiān)谟嗀浖?xì)胞時(shí)就向供貨商索取詳細(xì)的復(fù)蘇過(guò)程，并仔細(xì)閱讀。有些供貨商就不引薦在復(fù)蘇時(shí)離心，對(duì)此類細(xì)節(jié)，應(yīng)特別留心。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">&bull; </span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">儲(chǔ)存在冰箱中的瓶口已開(kāi)的培育基，或許在放置幾天后顏色變紫。這主要是由于在露出到周圍的<font face="Calibri">CO2</font><font face="宋體">水平常，碳酸氫納導(dǎo)致了</font><font face="Calibri">pH</font><font face="宋體">值的上升。您能夠在運(yùn)用前松開(kāi)瓶口，在</font><font face="Calibri">CO2</font><font face="宋體">培育箱孵育培育基</font><font face="Calibri">10-15</font><font face="宋體">分鐘，來(lái)校對(duì)溶液的</font><font face="Calibri">pH</font><font face="宋體">值</font><font face="Calibri">(</font><font face="宋體">確認(rèn)松開(kāi)瓶口以保證氣體交流</font><font face="Calibri">)</font><font face="宋體">。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">當(dāng)在無(wú)血清培育基中添加抗生素時(shí)，下降至少在有血清培育基中所運(yùn)用濃度的</font>50%<font face="宋體">。血清蛋白會(huì)結(jié)合和滅活一些抗生素。在無(wú)血清培育條件下，抗生素不被滅活，或許對(duì)于細(xì)胞到達(dá)毒性水平。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">一旦您在新鮮培育基中添加了血清和抗生素時(shí)，您應(yīng)該在兩到三周內(nèi)運(yùn)用它。由于一些抗生素和血清中的基本成分在凍結(jié)后就開(kāi)端降解。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">大部分添加物和試劑</font>zui<font face="宋體">多能夠凍融</font><font face="Calibri">3</font><font face="宋體">次，假如次數(shù)更多都會(huì)在包含蛋白的溶液引起一定水平的降解和沉積，將會(huì)影響它的性能。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">血清的熱滅活在免疫分析時(shí)能夠滅活補(bǔ)體體系。在免疫學(xué)研究，培育</font>ES<font face="宋體">細(xì)胞，昆蟲(chóng)細(xì)胞平和滑肌細(xì)胞時(shí)，引薦運(yùn)用熱滅活血清。熱滅活是以往公認(rèn)的操作過(guò)程，并沒(méi)有確鑿的依據(jù)說(shuō)明這樣做是有利的。相反，對(duì)大部分細(xì)胞而言，</font><font face="Calibri">GIBCO</font><font face="宋體">和</font><font face="Calibri">HyClone</font><font face="宋體">都不引薦熱滅活血清。由于加熱或許使血清中的沉積增加，使您誤以為污染。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">如何防止血清中沉積物的發(fā)生</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1.</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> <font face="宋體">凍結(jié)血清時(shí)，請(qǐng)依照所建議的逐步凍結(jié)法</font><font face="Calibri">(-20</font><font face="宋體">℃至</font><font face="Calibri">4</font><font face="宋體">℃至室溫</font><font face="Calibri">)</font><font face="宋體">，若血清凍結(jié)時(shí)改動(dòng)的溫度太大</font><font face="Calibri">(</font><font face="宋體">如</font><font face="Calibri">-20</font><font face="宋體">℃至</font><font face="Calibri">37</font><font face="宋體">℃</font><font face="Calibri">)</font><font face="宋體">，試驗(yàn)顯現(xiàn)十分簡(jiǎn)單發(fā)生沉積物。并隨時(shí)將之搖晃均勻，使溫度及成分均一，削減沉積的發(fā)生。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2. </span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">請(qǐng)勿將血清置于</font><font face="Calibri">37</font><font face="宋體">℃太久。若在</font><font face="Calibri">37</font><font face="宋體">℃放置太久，血清會(huì)變得混濁，一起血清中許多較不穩(wěn)定的成分也會(huì)因而受到損害，而影響血清的質(zhì)量。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3. </span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">血清的熱滅活十分簡(jiǎn)單形成沉積物的增多，若非必要，能夠無(wú)須做此過(guò)程。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4. </span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">若有必要做血清的熱滅活，請(qǐng)恪守</font><font face="Calibri">56</font><font face="宋體">℃，</font><font face="Calibri">30</font><font face="宋體">分鐘的原則，而且隨時(shí)搖晃均勻。溫度過(guò)高，時(shí)間過(guò)久或搖晃不均勻，都會(huì)形成沉積物的增多。 </font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">酶聯(lián)生物培育基價(jià)格質(zhì)量雙重保障，新老客戶均可享用優(yōu)惠，歡迎。</span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>