什么是ELISA微孔板？一文帶您了解類型、規(guī)格和應(yīng)用
<p> <font face="宋體" style="font-size: 10.5pt;"> 酶聯(lián)免疫吸附實(shí)驗(yàn)（</font><span style="font-family: Calibri; font-size: 10.5pt;">ELISA</span><font face="宋體" style="font-size: 10.5pt;">）是一種常用的基于板的免疫學(xué)領(lǐng)域金標(biāo)準(zhǔn)測(cè)定法，用于生命科學(xué)和分子生物學(xué)實(shí)驗(yàn)檢測(cè)和量化復(fù)雜混合物中的生物分子。</font></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 在技術(shù)上，將抗原或抗體包被在</font>96<font face="宋體">或</font><font face="Calibri">384ELISA</font><font face="宋體">微孔板的表面。然后，將微孔板與抗原特異性抗體一起孵育，這些抗體會(huì)與微孔板底部目標(biāo)抗原結(jié)合。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">板是</font><font face="Calibri">ELISA</font><font face="宋體">檢測(cè)的核心。它們也被稱為微量滴定板或微孔板，由聚苯乙烯或聚丙烯制成。如今，市場(chǎng)上有各種</font><font face="Calibri">ELISA</font><font face="宋體">板或微孔板可供選擇，以適應(yīng)不同的實(shí)驗(yàn)室工作流程。因此，要開(kāi)發(fā)精確、準(zhǔn)確和靈敏的生物測(cè)定，選擇合適的板至關(guān)重要，從而選擇合適的表面來(lái)固定目標(biāo)生物分子。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 在本文中，酶聯(lián)生物將為您介紹下</font>ELISA<font face="宋體">微孔板特點(diǎn)、市場(chǎng)上可買到的微孔板的格式及其多種應(yīng)用。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>Elisa<font face="宋體">板類型</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 微孔板有多種類型，每種類型都針對(duì)特定的檢測(cè)要求和檢測(cè)方法量身定制。每個(gè)微孔板的表面都對(duì)特定生物分子具有高結(jié)合親和力。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<ol>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 親和免疫分析板</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 親和免疫測(cè)定板是為了增強(qiáng)抗原和抗體的結(jié)合而開(kāi)發(fā)的。它為固定生物分子提供了高親和力的表面，從而提高了測(cè)定靈敏度、定量準(zhǔn)確度和最佳相互作用。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 這些板用于</font>ELISA<font face="宋體">和免疫測(cè)定。它們涂有鎳、鏈霉親和素、抗體和其他配體。它有多種類型，包括：</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 生物素結(jié)合板：包括</font>NeutrAvidin<font face="宋體">包被板和鏈霉親和素包被板。這些板通常用于以生物素化分子（如抗體或核酸）作為探針或檢測(cè)劑的檢測(cè)。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 抗體結(jié)合板：抗體結(jié)合板的類型包括蛋白</font>A/G/L<font face="宋體">包被板和二抗（</font><font face="Calibri">GAM</font><font face="宋體">、</font><font face="Calibri">GAR</font><font face="宋體">）包被板。它們與各種類型的免疫球蛋白（</font><font face="Calibri">IgG</font><font face="宋體">、</font><font face="Calibri">IgM</font><font face="宋體">和</font><font face="Calibri">IgA</font><font face="宋體">等）和一抗結(jié)合，用于高效抗原檢測(cè)。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>His<font face="宋體">標(biāo)簽結(jié)合板：鎳和銅涂層板是</font><font face="Calibri">His</font><font face="宋體">標(biāo)簽結(jié)合板的幾種類型。它們廣泛用于涉及組氨酸標(biāo)記蛋白的測(cè)定。</font><font face="Calibri">His</font><font face="宋體">標(biāo)簽結(jié)合板可實(shí)現(xiàn)</font><font face="Calibri">His</font><font face="宋體">標(biāo)簽蛋白的特異性固定和檢測(cè)。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>GST<font face="宋體">結(jié)合板：包括谷胱甘肽</font><font face="Calibri">(GSH)</font><font face="宋體">涂層板，用于檢測(cè)和分析</font><font face="Calibri">GST</font><font face="宋體">標(biāo)記或谷胱甘肽</font><font face="Calibri">S-</font><font face="宋體">轉(zhuǎn)移酶</font><font face="Calibri">(GST)</font><font face="宋體">融合蛋白。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
</ol>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong><font face="宋體">吸收免疫分析板</font></strong></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 吸收免疫測(cè)定板也稱為結(jié)合免疫測(cè)定板。這些板是為蛋白質(zhì)、抗體和其他生物分子的被動(dòng)吸附（包被）而開(kāi)發(fā)的。它們被設(shè)計(jì)為專門針對(duì)特點(diǎn)的抗原。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 它也有兩種類型：</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<ol>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 親水性蛋白質(zhì)結(jié)合板：它們旨在吸收親水性的生物分子，例如糖蛋白、抗體、聚糖和其他可溶性蛋白質(zhì)。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 疏水性脂質(zhì)結(jié)合板：它們被設(shè)計(jì)用于吸收疏水性的生物分子，例如脂質(zhì)和脂蛋白。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
</ol>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> <strong>共價(jià)免疫分析板</strong></font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 這些專門設(shè)計(jì)的微孔板可實(shí)現(xiàn)肽、核酸和其他生物分子的共價(jià)連接。它們能夠與各種功能基團(tuán)結(jié)合，包括氨基</font>(NH2)<font face="宋體">、巰基</font><font face="Calibri">(SH)</font><font face="宋體">、羧基</font><font face="Calibri">(COOH)</font><font face="宋體">和磷酸基</font><font face="Calibri">(PO4)</font><font face="宋體">，從而實(shí)現(xiàn)穩(wěn)定而高效的固定。它們可用于</font><font face="Calibri">ELISA</font><font face="宋體">和其他基于板的檢測(cè)技術(shù)。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 共價(jià)免疫測(cè)定板的一個(gè)例子是核酸測(cè)定板。它們有多種類型，以適應(yīng)寡核苷酸與各種功能基團(tuán)或標(biāo)簽的結(jié)合。這些板可以通過(guò)共價(jià)鍵或親和相互作用實(shí)現(xiàn)牢固連接，確保在實(shí)驗(yàn)中準(zhǔn)確可靠地分析核酸。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>Elisa<font face="宋體">板工作原理</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">板是免疫學(xué)和診斷實(shí)驗(yàn)室中定期進(jìn)行</font><font face="Calibri">ELISA</font><font face="宋體">檢測(cè)的必備材料。它確保目標(biāo)抗原的結(jié)合并保持目標(biāo)分析物和抗體之間的化學(xué)反應(yīng)。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 為了開(kāi)發(fā)有效的免疫測(cè)定法，用適當(dāng)?shù)牡鞍踪|(zhì)或抗體包被</font>ELISA<font face="宋體">板至關(guān)重要。根據(jù)所需的檢測(cè)信號(hào)選擇合適的微孔板類型對(duì)于實(shí)現(xiàn)最佳的板包被至關(guān)重要。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 檢測(cè)微孔板顏色的選擇取決于所檢測(cè)的信號(hào)。例如，黑色或白色不透明板用于檢測(cè)化學(xué)發(fā)光和熒光信號(hào)，而透明聚苯乙烯平底板用于檢測(cè)比色信號(hào)。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 確保板上沒(méi)有劃痕，以免干擾分析和最終結(jié)果。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 包被過(guò)程涉及被動(dòng)吸附，即將蛋白質(zhì)或抗體添加到板中，并讓其與塑料表面結(jié)合。通常，先制備蛋白質(zhì)或抗體溶液，然后在板上孵育特定時(shí)間。孵育后，除去包被溶液，并添加封閉緩沖液以防止非特異性結(jié)合。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 需要注意的是，理想包被條件和結(jié)合能力可能因所用的特定蛋白質(zhì)或抗體而異。通常需要通過(guò)實(shí)驗(yàn)來(lái)確定適當(dāng)?shù)陌粷舛?。包被板的蛋白質(zhì)含量略高于所需量可實(shí)現(xiàn)更廣泛的檢測(cè)范圍，但包被量過(guò)大可能導(dǎo)致非特異性結(jié)合和高背景信號(hào)。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 板子包被后，可立即使用或儲(chǔ)存在</font>4°C<font face="宋體">以備將來(lái)使用，具體取決于包被蛋白的穩(wěn)定性。適當(dāng)?shù)膬?chǔ)存條件可確保檢測(cè)結(jié)果的可靠性和可重復(fù)性。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">板用途</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">聚苯乙烯微孔板在研究、臨床和診斷實(shí)驗(yàn)室及行業(yè)中有著廣泛的應(yīng)用。它使科研人員能夠量化樣本中目標(biāo)分子的存在和濃度，例如血液、血清、血漿、尿液或細(xì)胞裂解物。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 這些板具有多個(gè)孔或隔間，可同時(shí)評(píng)估多個(gè)樣品或測(cè)試條件。它們可進(jìn)行高通量篩選，使研究人員能夠高效處理多個(gè)樣品。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">板兼容各種檢測(cè)方法，包括比色、熒光和化學(xué)發(fā)光技術(shù)，為檢測(cè)和測(cè)量感興趣的抗原提供了靈活性。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong>ELISA<font face="宋體">檢測(cè)</font></strong></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">是實(shí)驗(yàn)室中最常見(jiàn)和最廣泛使用的基于板的免疫測(cè)定技術(shù)之一。除了微量離心管和移液器外，該測(cè)定還需要</font><font face="Calibri">8</font><font face="宋體">孔條、</font><font face="Calibri">96</font><font face="宋體">孔或</font><font face="Calibri">384</font><font face="宋體">孔</font><font face="Calibri">ELISA</font><font face="宋體">中結(jié)合或高結(jié)合板。它們?cè)试S同時(shí)評(píng)估多種生物分子。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 對(duì)于從事組織培養(yǎng)或基于細(xì)胞的分析的研究人員來(lái)說(shuō)，介質(zhì)結(jié)合</font>ELISA<font face="宋體">板為細(xì)胞粘附和生長(zhǎng)提供了最佳表面。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 孵育和清洗培養(yǎng)板時(shí)，必須正確密封培養(yǎng)板，以防止交叉污染并保持樣品的完整性。培養(yǎng)板密封器可以簡(jiǎn)化此過(guò)程。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 使用</font>ELISA<font face="宋體">板時(shí)，請(qǐng)考慮用于產(chǎn)生信號(hào)的底物。不同的底物提供各種檢測(cè)選項(xiàng)，包括比色和化學(xué)發(fā)光信號(hào)，讓您可以根據(jù)實(shí)驗(yàn)需要選擇最合適的方法。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 以上就是關(guān)于</font>ELISA<font face="宋體">微孔板的介紹。選擇合適的微孔板對(duì)</font><font face="Calibri">ELISA</font><font face="宋體">實(shí)驗(yàn)結(jié)果至關(guān)重要，建議根據(jù)實(shí)驗(yàn)需求，例如結(jié)合類型和材質(zhì)，仔細(xì)選擇合適的</font><font face="Calibri">ELISA</font><font face="宋體">微孔板，以確保實(shí)驗(yàn)的成功。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
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